Improvement of Chronic Wound Healing by Pre-activated Bone Marrow Cells with Sodium Nitroprusside in Rabbits

Abstract

Aim of study This study investigated whether pre-activated bone marrow cells with sodium nitro prusside have effectiveness in the inhibition of diabetic wound healing in diabetic rabbits. In diabetic skin disorders and conditions involved redox state disturbances. The aim was to determine the effect of two minimum dosages of sodium nitro prusside, and its’ potential with bone marrow cells for chronic wound healing in-vivo. Methods Full-thickness skin dorsal wounds were created on diabetic rabbits. The effects of two minimum concentrations of sodium nitro prusside solution with bone marrow cells on wound healing were studied. The useful combination of sodium nitro prusside with bone marrow cells on wound repair may be attributed to its functional influences on inflammation, angiogenesis, cell proliferation, matrix deposition, and remodeling. Results The in-vivo experiments confirmed that pre-activated bone marrow cells contributed to wound healing by alleviating oxidative stress, increasing proliferation and migration, decreasing apoptosis. In histological results, improved collagen deposition, enhanced re-epithelization, angiogenesis, and decreased inflammatory infiltration were also detected in wound biopsies. Conclusions For the treatment of chronic wounds, cell-based therapy was an attractive approach. Bone marrow cells have a low ability to differentiate various types of cells or late healing without pretreatment. So it was needed to increase their potency of differentiation. The transplantation of pretreated bone marrow cells with a prime quantity of sodium nitro prusside solution improved chronic wound healing with a greater level of growth factors and a minimum level of oxidative stress.

Nitroprusside and metal nitroprusside nano analogues for cancer therapy

“…SNP has various biomedical applications such as cardiovascular, ischemic disease, preeclampsia, angiogenesis, wound healing and neurological disorders. These applications are subsequently discussed in the following sections [25,28,29,51,52].…”

“…Wound healing is a highly programmed and regulated biological process which involves various steps such as homeostasis, inflammation, proliferation and remodeling. Delayed long term wound healing is a major problem in diabetic patients which is attributed to inadequate NO release, lack of oxygen supply and growth factors secretion as well as inhibition of angiogenic program [28]. To this, Fatima et al used SNP for the pre-activation bone marrow cells, which improves the chronic wound healing in diabetic rabbits.…”

“…The SNP activated bone marrow cells effectively alleviate oxidative stress in the wounded area and encompassing the migration, proliferation and inhibition of apoptotic cell death. The major reason involved in the healing process includes angiogenesis, collagen deposition etc [28]. Apart from this, there are also few reports which demonstrated the wound healing property of SNP in plants.…”

“…The immense potential of this compound mainly relies on its unique physiochemical properties such as quick action, short half life, water solubility, rapid break down in the blood stream and neutral effect on cardiac function [15-18, 24, 25]. The other biological applications of SNP include angiogenesis, wound healing, neurological disorders and cancer [8,[26][27][28][29]. Moreover, as a well-known NO donor compound, recently SNP is extensively used for cancer therapy [8,[30][31][32][33].…”

Effect of Taspine hydrochloride on the repair of rat skin wounds by regulating keratinocyte growth factor signal

“…With economic development and social progress in recent years, the number of traumatized people has become larger and larger, and people have paid more and more attention to and discussed the wound repair. As the largest external organ in the body and the first barrier to protect the body, the skin is most vulnerable to trauma [ 1 ]. Skin wounds repair refers to the interaction of various proliferating repair cells, intercellular substance, and growth factors after skin injury, filling and connecting the damaged tissues, thereby completing the complex dynamic process of self-repair [ 2–4 ].…”