Improvement of chiral separation efficiency through temperature control during one time high performance liquid chromatography analysis

Abstract

This study examined how manual temperature control affects the separation of two enantiomers of five racemic mixtures using high‐performance liquid chromatography (HPLC) in terms of separation factor (α), resolution (Rs), and the number of theoretical plates (N). The results showed that heating/cooling during one time HPLC analysis improved the separation factor and resolution.

Use of ultrasound in gradient elution of Polycyclic Aromatic Hydrocarbon mixtures by HPLC

“…Subsequently, the ultrasonic device was turned off after the elution of the first peak, and the separation factor between the two enantiomer peaks increased in the absence of a temperature increase 7 . Our recent findings confirmed that the resolution and separation factor of enantiomers in chiral separation can be improved by controlling the bath temperature using the one‐time HPLC with the SS chiral column 2 . To investigate the extent to which sono‐gradient elution could reduce the retention time, the SS and PEEK columns were irradiated with ultrasonic intensities of 0%, 10%, 20%, 30%, 60%, and 100% of 900 W under constant and inconstant temperature conditions after pyrene was eluted.…”

“…7 Our recent findings confirmed that the resolution and separation factor of enantiomers in chiral separation can be improved by controlling the bath temperature using the one-time HPLC with the SS chiral column. 2 To investigate the extent to which sono-gradient elution could reduce the retention time, the SS and PEEK columns were irradiated with ultrasonic intensities of 0%, 10%, 20%, 30%, 60%, and 100% of 900 W under constant and inconstant temperature conditions after pyrene was eluted. The retention time of benzo[α]pyrene is presented in Table 2.…”

“…Various external factors can be used to adjust the retention of a sample in chromatography without changing the stationary or mobile phases. These external chromatographic factors include temperature, 1,2 ultrasound, [3][4][5][6][7][8] magnetic force, 9 and optical force. 10 In particular, to separate complex mixtures, various factors can be used for an effective separation via gradient methods in gas chromatography, 1 supercritical fluid chromatography, 11 and high-performance liquid chromatography (HPLC).…”