Improvement of chemical analysis of antibiotics. 21. Simultaneous determination of three polyether antibiotics in feeds using high-performance liquid chromatography with fluorescence detection

Asukabe, Hirohiko; Murata, Hideaki; Harada, Kenichi; Suzuki, Makoto; Oka, Hisao; Ikai, Yoshitomo

doi:10.1021/jf00037a019

Cited by 29 publications

(16 citation statements)

References 15 publications

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“…The use of enzyme-linked immunosorbentassay (ELISA) has also been explored as an alternative to existing chromatographic techniques for the detection of salinomycin in various biological matrices (Elissalde et al 1993, Muldoon et al 1995. Since SAL lacks a chromophore for detection by spectrophotometry, it is detected after post-column derivatization with vanillin (Blanchflower et al 1985, Lapointe and Cohen 1988, Asukabe et al 1994 or with 4-dimethylaminobenzaldehyde (Fejglova et al 1994). The post-column derivatization is performed between 85 and 95°C.…”

Section: Introductionmentioning

confidence: 99%

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Akhtar

El‐Sooud

Shehata

1996

Food Additives and Contaminants

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Laying chickens were fed medicated feed containing various concentrations of sodium salinomycin (SAL) for 14 days followed by a 3 day withdrawal period. Eggs, collected during treatment and withdrawal, tissues and ovarian yolk of birds slaughtered after 0, 1, and 3 days' withdrawal were extracted and analysed by high performance liquid chromatography (HPLC). Tissues, ovarian yolk and freeze-dried egg albumen and yolk were extracted with acetone, followed by partitioning with petroleum ether and HPLC analysis. Albumen was extracted with methanol and analysed without further clean-up. Salinomycin was detected at 520 nm after post-column reaction with vanillin at 95 degrees C. Recoveries of fortified salinomycin from freeze-dried eggs (albumen and yolk) and tissue, premix and feed were nearly quantitative (> 90%), except liver which was < 85%. The detection limit was estimated to be 5 ng g-1, with the practical quantifiable limit being about 10 ng g-1. Highest SAL concentrations were in the more fatty components such as egg yolk, ovarian yolk and subcutaneous fat. SAL residues in other tissues were generally low and followed the order liver, kidney, thigh and breast muscles. SAL residues were dependent on the SAL concentration in feed and declined rapidly during withdrawal.

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Section: Introductionmentioning

confidence: 99%

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Akhtar

El‐Sooud

Shehata

1996

Food Additives and Contaminants

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“…In order to monitor MD levels in biological matrixes, a number of analytical methods, such as high-performance liquid chromatography (HPLC) (Gliddon et al 1988;Markantonatos 1988;Johnson 1989;Asukabe et al 1994;Dubois, Pierret, and Delahaut 2004), and immunoassays (Wong 1990;Kennedy, Blanchflower, and O'Dornan 1997;Shen et al 2001;Wang et al 2008), have been developed. As HPLC methods require expensive instrumentation, highly skilled personnel, and extensive sample clean-up, they are not suitable for routine analysis …”

Section: Introductionmentioning

confidence: 99%

Monoclonal Antibody-Based Immunoassay for the Detection of Maduramicin in Chicken Tissues

et al. 2009

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This paper reports on the preparation of a monoclonal antibody (Mab) against maduramicin (MD) and the development of a simple and sensitive ELISA for MD in chicken tissues. MD was conjugated to bovine serum albumin for immunogens and ovalbumin for coating antigens by mixed anhydride (MA) and active ester (AE) methods. Hybridoma cells were generated using spleen cells from a mouse immunized with MD-BSA conjugate. After screening with ELISA, the Mab with high anti-interference ability and high affinity was selected, and it exhibited negligible cross-reactivity with other usual-used polyether antibiotics. After optimization, the developed ELISA showed an IC 50 value of 2.12 AE 0.46 ng=ml (n ¼ 20). Chicken muscle and liver samples were extracted with methanol-8% NaCl solution (4:1) and then directly diluted with PBS-10% methanol for analysis. The recoveries of MD from spiked chicken tissues at levels of 40-480 mg=kg ranged from 81.3% to 91.3% with variation coefficients of

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“…To determine monensin in different biological matrices, several analytical methods have been used. These methods are based on colorimetry [5], high performance liquid chromatography with UV [6,7] or fluorescence detection [8,9] and liquid chromatography linked with mass spectrometry [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

Chéneau

Henri

Pirotais

et al. 2007

Journal of Chromatography B

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Improvement of chemical analysis of antibiotics. 21. Simultaneous determination of three polyether antibiotics in feeds using high-performance liquid chromatography with fluorescence detection

Cited by 29 publications

References 15 publications

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Monoclonal Antibody-Based Immunoassay for the Detection of Maduramicin in Chicken Tissues

Liquid chromatography–electrospray tandem mass spectrometric method for quantification of monensin in plasma and edible tissues of chicken used in pharmacokinetic studies: Applying a total error approach

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