Improvement of Cauliflower Male Sterile Lines with Brassica Nigra Cytoplasm, Phenotypic Expression and Possibility of Practical Application

Kamiński, Piotr; Dyki, B.; Stępowska, A.

doi:10.5539/jas.v4n4p190

Cited by 8 publications

(13 citation statements)

References 14 publications

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“…Homeotic conversion of stamens into carpelloid structures were described in several CMS lines of wheat (Ogihara et al, 1997), tobacco (Farbos et al, 2001), carrot (Linke et al, 2003), rapeseed (Leino et al, 2003), cauliflower (Kamiñski et al, 2012), and broccoli (Shu et al, 2015). Stamens of the CMS lines were replaced by carpelloid organs, thus resembled the B-class genes APETALA3 ( AP3 ) and PISTILLATA ( PI ) mutants.…”

Section: Discussionmentioning

confidence: 99%

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

Kang

Wang

et al. 2017

Front. Plant Sci.

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A novel cytoplasmic male sterility (CMS) in Brassica napus (inap CMS) was selected from the somatic hybrid with Isatis indigotica (Chinese woad) by recurrent backcrossing. The male sterility was caused by the conversion of tetradynamous stamens into carpelloid structures with stigmatoid tissues at their tips and ovule-like tissues in the margins, and the two shorter stamens into filaments without anthers. The feminized development of the stamens resulted in the complete lack of pollen grains, which was stable in different years and environments. The pistils of inap CMS displayed normal morphology and good seed-set after pollinated by B. napus. Histological sections showed that the developmental alteration of the stamens initiated at the stage of stamen primordium differentiation. AFLP analysis of the nuclear genomic composition with 23 pairs of selective primers detected no woad DNA bands in inap CMS. Twenty out of 25 mitochondrial genes originated from I. indigotica, except for cox2-2 which was the recombinant between cox2 from woad and cox2-2 from rapeseed. The novel cox2-2 was transcribed in flower buds of inap CMS weakly and comparatively with the fertile B. napus addition line Me harboring one particular woad chromosome. The restorers of other autoplasmic and alloplasmic CMS systems in rapeseed failed to restore the fertility of inap CMS and the screening of B. napus wide resources found no fertility restoration variety, showing its distinct origin and the related mechanism of sterility. The reasons for the mitochondrial rearrangements and the breeding of the restorer for the novel CMS system were discussed.

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Section: Discussionmentioning

confidence: 99%

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

Kang

Wang

et al. 2017

Front. Plant Sci.

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“…The significance of differences among means was evaluated by Tukey's test at p = 0.05. A correlation Kamiński et al 2012). Generally, CMS genotypes with deleterious drawbacks for vegetative growth or seed production should not be used for breeding (Budar et al 2006).…”

Section: Evaluation Of Agronomical and Commercial Traits Of Male-stermentioning

confidence: 99%

“…This procedure allowed the identification of desired rf genotypes. (HoserKrauze 1987, Kamiński et al 2012.…”

Section: Introductionmentioning

confidence: 99%

“…A lack of rigorous selection in the early generations following fusion or sexual hybridisation may cause problems with the failure of seed production among male-sterile plants (Dickson and Kyle 1987). The selection of CMS cauliflowers with the B. nigra cytoplasm performed in the former Research Institute of Vegetable Crops in Skierniewice resulted in obtaining genotypes with higher ability for generative propagation and better quality of commercial characteristics (Kamiński et al 2012). The B. nigra system in contrast to Ogu-INRA allows for the development of fertile F 1 hybrids for B. oleracea genotypes, as all forms with fertile cabbage cytoplasm act as fertility restorers.…”

Section: Introductionmentioning

confidence: 99%

“…(Kamiński et al 2012). Three fertile inbred lines of the F 5 generation (FL, FA43, DT70) and two male-sterile lines after BC 5 with the Ogu-INRA cytoplasm (CFA43, CDT70) were also used.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The generative propagation and quality of new male-sterile cauliflowers with the Ogu-INRA and Brassica nigra cytoplasm

Kamiński

2014

Folia Horticulturae

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In the years of 2012-2013 male-sterile cauliflower genotypes with the Brassica nigra cytoplasm and their maintainers were tested with respect to their ability for generative propagation and the quality of agronomical traits in comparison to male-fertile inbreeds and sterile genotypes with the Ogu-INRA cytoplasm. The ability for the generative propagation for male-sterile genotypes with the B. nigra cytoplasm was diversified and lower than for the maintainer, fertile lines and lines with the Ogu-INRA cytoplasm.Generative stalks of cauliflowers with the B. nigra cytoplasm usually had a higher number of buds and flowers in comparison to the fertile genotypes and to the sterile Ogu-INRA lines. The most desired commercial characteristics, such as circular shape, compactness of curd and intermediate or strong coverage by leaves, were noticed for sterile lines with the Ogu-INRA cytoplasm and their fertile complementary lines. Three experimental F 1 hybrids showed good quality in comparison to commercial F 1 cultivars.

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The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

Colombo¹,

Galmarini

2017

Plant Breeding

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Production of hybrid varieties of vegetable crops is currently a desired breeding goal, due to their remarkable agronomic performance and to the possibility of intellectual property protection. However, efficient hybrid production requires a careful pollination control to guarantee the hybrid nature of F 1 seed. Several technologies ranging from manual emasculation to genetic transformation are used to inhibit pollen production in mother plants. In this review, we examine the principles underlying strategies like genetically determined systems (genic male sterility, cytoplasmic-genic male sterility, self-incompatibility) and other methods (manual emasculation, chemical-hybridizing agents) in different species, considering the benefits and drawbacks of their adoption. Finally, we present the current state of the art for vegetable hybrid seed production.

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Improvement of Cauliflower Male Sterile Lines with Brassica Nigra Cytoplasm, Phenotypic Expression and Possibility of Practical Application

Cited by 8 publications

References 14 publications

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

The generative propagation and quality of new male-sterile cauliflowers with the Ogu-INRA and Brassica nigra cytoplasm

The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review

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