Improvement of a two-stage fermentation process for docosahexaenoic acid production by Aurantiochytrium limacinum SR21 applying statistical experimental designs and data analysis

In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp. by 18S rRNA sequencing and named KRS101 used various carbon and nitrogen sources, indicating metabolic versatility. Optimal culture conditions, thus maximizing cell growth, and high levels of lipid and DHA production, were attained using glucose (60 g l⁻¹) as carbon source, corn steep solid (10 g l⁻¹) as nitrogen source, and sea salt (15 g l⁻¹). The highest biomass, lipid, and DHA production of KRS101 upon fed-batch fermentation were 50.2 g l⁻¹ (16.7 g l⁻¹ day⁻¹), 21.8 g l⁻¹ (44% DCW), and 8.8 g l⁻¹ (40% TFA), respectively. Similar values were obtained when a cheap substrate like molasses, rather than glucose, was used as the carbon source (DCW of 52.44 g l⁻¹, lipid and DHA levels of 20.2 and 8.83 g l⁻¹, respectively), indicating that production of microbial oils containing high levels of DHA can be produced economically when the novel strain is used.

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“…These production values of Aurantiochytrium sp. KRS101 are similar to those reported elsewhere [29][30][31].…”

Section: Fed-batch Fermentationsupporting

confidence: 89%

Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101

Hong

Rairakhwada

Seo

et al. 2011

Appl Biochem Biotechnol

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“…In fact, the majority of the product MPA was produced during this period. Therefore, the feeding strategy could be significantly increased by extending the production period and shortening the initial growth phase [20].…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the control strategy for MPA high-density fermentation is to extend the equilibrium phase and slow down the cell autolysis. Feeding strategy is the most important means of achieving high-density fermentation [19][20][21][22]. There are few reports of feeding strategies for enhancing MPA production.…”

Section: Introductionmentioning

confidence: 99%

A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum

Dong

Wang

et al. 2015

Process Biochemistry

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“…CCTCC M209059. Positive effect of yeast extract on DHA production is likely due to supply of vitamins from this component which favour biomass production and consequently the absolute amount of DHA accumulated [19].…”

Section: Resultsmentioning

confidence: 99%

Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1

Manikan

Hamid²

2013

AIP Conference Proceedings

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Central composite design (CCD) was employed to investigate the significance of glucose, yeast extract, MSG and sea salt in affecting the amount of docosahexaenoic acid (DHA) accumulated by a locally isolated strain of Schizochytrium. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over three levels. Cultivation was carried out in 250mL flasks containing 50mL of medium, incubated at 30°C with 200 rpm agitation for 96 hours. ANOVA was conducted to identify the influential factors and the level of their significance where factors that scored a probability value of less than 0.05 were considered significant. The level of influence for each independent variable was also interpreted using perturbation whereas pattern of interaction between the factors were interpreted using interaction plots. This experiment revealed that yeast extract and monosodium glutamate have significant influence on DHA accumulation process by Schizochytrium sp. SW1.

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Improvement of a two-stage fermentation process for docosahexaenoic acid production by Aurantiochytrium limacinum SR21 applying statistical experimental designs and data analysis

Cited by 62 publications

References 27 publications

Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101

Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101

A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum

Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1

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