Improvement in Nutrient Digestibility and Growth Performance of Catla catla Fingerlings Using Phytase in Moringa oleifera Leaf Meal Based Diet

Shahzad, Muhammad Mudassar; Hussain, Syed Makhdoom; Akram, Afia Muhammad; Javid, Arshad; Hussain, Majid; Shah, Syed Zakir Hussain; Chaudhary, Asma

doi:10.17582/journal.pjz/2020.52.1.157.168

Cited by 4 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly Ehsani and Torki (2010) also found that the addition of PHY stimulates the immune system of fish producing high number of macrophages. With agreement of current study, Shahzad et al (2019;2020) also noted closely similar results. They observed high contents of RBCs and Hb in Catla catla were observed in diet having 900 FTU per kg PHY level.…”

Section: Resultssupporting

confidence: 91%

See 1 more Smart Citation

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

2020

THE JAPS

View full text Add to dashboard Cite

Increasing human population and lower food availability motivated the researchers to search out the alternative and low cost feed sources for fish that is the basic protein source in human diet. For this purpose, study was performed to develop an eco-friendly and low-cost feed for 'Nile tilapia' fingerlings using Moringa derived products supplemented with phytase (PHY) enzyme. Moringa oleifera seed meal and leaf meal were used as the major components to make seven iso-nitrogenous and iso-caloric test diets (TD). These Moringa based test diets were developed by using different PHY levels (0 control, 500, 650, 800, 950, 1100 and 1250 FTU per kg). Seven groups (in three replicates) of tilapia fingerlings were fed at the rate of 4% of their live weight two times a day. After 70 days feeding trials, blood samples from each replicate were collected for the analysis of hemato-immunological parameters. Then these fish were sacrificed and dried for carcass analysis. After the application of one-way ANOVA, maximum retention of protein (18.26%) and fat (8.92) were found at 950 FTU per kg in comparison to other test diets. Study of hematological indices revealed that the maximal values of RBCs (2.92×10 6 mm-3), PLT (64) and Hb (8.19 g/100ml) were recorded at 950 FTU per kg level. Similarly, highest MCH, PCV, MCV and Ht. were also observed at 950 FTU per kg level. For the study of expression of growth genes, gel electrophoresis method was performed. It was concluded that PHY added diet at 950 FTU per kg showed maximum improvement in hemato-immunological parameters and body composition of tilapia with no side effects on fish health and non-differentiable results regarding growth gene expression.

show abstract

Section: Resultssupporting

confidence: 91%

“…Pellets Formation and feeding: Collected Moringa leaves and seeds were crushed and ground as fine powder after their processing as described by Shahzad et al (2019). Proximate chemical composition of all feed ingredients was done (AOAC 1995) before pellet formation (Table 1).…”

Section: Experimental Designmentioning

confidence: 99%

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

2020

THE JAPS

View full text Add to dashboard Cite

show abstract

“…According to the present results, level of 900, FTU/kg was the optimal phytase inclusion for the maximum amelioration in the nutrient digestibility in contrast with other test and non-supplemented diet. Likewise, in one of the studies, maximum improvement in nutrient digestibility was examined in C. catla fingerlings fed phytase substituted MOSM based diet ( Hussain et al, 2017 , Shahzad et al, 2018 ) and MOLM based diet ( Shahzad et al, 2020 ). They found maximum CP (72%), GE (74%) and EE (80%) digestibility when the fingerlings were fed at 900, FTU/kg level-based diet.…”

Section: Discussionmentioning

confidence: 99%

Improvement in overall performance of Catla catla fingerlings fed phytase included low cost plant by products-based diet

Shahzad

Hussain

et al. 2020

Saudi Journal of Biological Sciences

Self Cite

View full text Add to dashboard Cite

Phytic acid’s presence in low-cost Moringa by-products effect the availability of important nutrients, diminishing the fish quality and blood composition in fish. Phytate having chelating effects with nutrients and minerals, can be reduced by the supplementation of phytase enzyme. Without the use of enzyme, plant meal may cause water pollution and decrease the fish health that results in higher culture cost. Therefore, current study was designed to check improvement in overall performance of Catla catla fingerlings fed Moringa by product-based diets supplemented with phytase (0, 300, 600, 900, 1200 and 1500, FTU/kg). All diets were integrated with non-digestible marker (Cr 2 O 3 ) at the rate of 1%. The fingerlings were fed couple of times a day (4% of live wet weight). Results showed significant ( p < 0.05 ) improvement in nutrient digestibility (i.e. EE, CP and GE), carcass composition and hematological parameters (i.e. RBCs, PLT and Hb) at 900, FTU/kg of phytase in contrast with other treatments. Moreover, phytase addition improves the water quality by reducing the nutrients leaching through feces at low cost. Current results indicated that, using mixture of Moringa seed meal and Moringa leaf meal based diet supplemented with phytase at 900, FTU/kg concentration is the most optimum level to develop a cost-effective as well as eco-friendly fish feed with maximum absorption of important nutrients and minerals in fish body resultantly high higher fish performance.

show abstract

“…The involvement of phytase in increasing protein digestibility is not direct, in fact, it is an outcome of phytase action on the digestibility of amino acid 48 . Phytase helps in retaining more nutrients inside the fish body 32,70,71,131 . Increased digestibility of minerals such as Fe, Mg, Ca, P, K, Zn, Cu, Na, Al, Mn, Sr, Cr and Pb has been observed in O .…”

Section: Nutrients Digestibilitymentioning

confidence: 99%

Role of microbial phytases in improving fish health

Priya

Virmani

Pragya

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Fishmeal is the most preferred protein source in aquafeed industry due to its balanced amino acid and fatty acid profile, but its limited global supply and high price value, necessitates researchers to focus on sustainable alternative sources of protein and plant‐based feed, to meet the growing demand of fish feed. Phytate is a reservoir of phosphorus present in the plants; however, its chelating ability makes it anti‐nutritional. Phytate forms complexes with minerals, proteins, lipids and carbohydrates, confining their accessibility in fish feeds, hence, additional dietary nutrients are required in the fish feed to overcome nutritional deficiencies. The elimination of phytate‐phosphorus through animal faeces and its pass on to aquatic bodies leads to enrichment of nutrients, eutrophication and algal blooms. Microbial phytase segregates phosphorus, minerals, proteins and lower inositol phosphates from phytate. The supplementation of fish feed with microbial phytase enhances the digestibility of minerals, due to which less nutrients are excreted in the faeces; therefore, minimizing pollution in water bodies. Monogastric and agastric animals inherently exhibit the deficiency of phytase; hence, supplementation of dietary material with phytase has been an emerging choice to make dietary nutrients available for fish. Moreover, microbial phytases have a broad pH range and are thermostable, which makes them an excellent choice for commercial fish feed production. Additionally, microbial phytase improves fish growth as these enzymes enhance nutrients bioavailability and their digestion. This review article presents the application of microbial phytases as a feed additive, and their role in enhancing fish growth.

show abstract

Improvement in Nutrient Digestibility and Growth Performance of Catla catla Fingerlings Using Phytase in Moringa oleifera Leaf Meal Based Diet

Cited by 4 publications

References 24 publications

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

EFFECT OF PHYTASE SUPPLEMENTED MORINGA BY-PRODUCTS BASED DIETS ON The PERFORMANCE OF OREOCHROMIS NILOTICUS FINGERLINGS

Improvement in overall performance of Catla catla fingerlings fed phytase included low cost plant by products-based diet

Role of microbial phytases in improving fish health

Contact Info

Product

Resources

About