Improvement in modal wavefront sensing in terms of cross-talk reduction and linearity

Konwar, Santanu; Boruah, Bosanta R.

doi:10.1117/12.2319928

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…[13][14][15] However, such sensors have to either undergoe extensive computation in order to obtain the final result 13,16 or suffers from inter-modal crosstalk. [17][18][19][20] Apart from wavefront sensors, the amplitude of defocus can also be measured using astigmatic lens. 21,22 The astigmatic lens approach gives a direct measure of the amplitude of defocus present in the beam, from the record of the intensities at the plane of measurement.…”

Section: Introductionmentioning

confidence: 99%

Direct defocus measurement using single astigmatic lens: a theoretical study

Konwar¹,

Kumar²,

Buddha³

2023

Photonic Instrumentation Engineering X

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Astigmatism has a very unique ability to change the shape of the intensity profile of an optical beam as we move away on either side of the focal plane of the optical system. This property of astigmatism can be used to measure the focussing error or the amplitude of defocus present at the plane of measurement. The use of astigmatism to measure focussing error is a very simple and easily implementable process. Astigmatism can be introduced with the help of an astigmatic lens. The approach provides a direct measure of the amplitude of defocus present in an optical beam from the measure of the intensity at the focal plane. In this paper we put forward a theoretical discussion on the above mentioned approach.

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Section: Introductionmentioning

confidence: 99%

Direct defocus measurement using single astigmatic lens: a theoretical study

Konwar¹,

Kumar²,

Buddha³

2023

Photonic Instrumentation Engineering X

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“…13 The 3D PSF of a widefield microscope can also be estimated without imaging any subresolution fluorescent bead using a Shack-Hartmann wavefront sensor by measuring the wavefront at the exit pupil of the image plane. [14][15][16][17][18][19][20] However, for a highly aberrated imaging system, the wavefront could not be measured accurately, which leads to approximation error in PSF estimation. The PSF of a widefield microscope can be estimated with adequate SNR by performing linear non-blind 3D deconvolution from the z-stack of a through a thin specimen.…”

Section: Introductionmentioning

confidence: 99%

Estimation of 3D point spread function of a widefield microscope by stochastic least square minimization method

Buddha¹,

Kumar²,

Konwar³

et al. 2023

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX

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The point-spread function (PSF) plays a fundamental role in deciding the resolution of an optical microscope. One way to find the experimental PSF is to image sub-resolution nanobead. However, the PSF can also be approximated numerically by knowing the optical arrangement of the imaging system. In this paper, we estimate the experimental three-dimensional PSF of a widefield microscope by taking several z-stacks of an arbitrary target, followed by application of our 2D PSF estimation scheme to each z slice. Here we use a standard resolution test target as the arbitrary target whose geometrical-optics predicted image can be easily constructed.

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Improvement in modal wavefront sensing in terms of cross-talk reduction and linearity

Cited by 2 publications

References 14 publications

Direct defocus measurement using single astigmatic lens: a theoretical study

Direct defocus measurement using single astigmatic lens: a theoretical study

Estimation of 3D point spread function of a widefield microscope by stochastic least square minimization method

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