Improvement in glucose biosensing response of electrochemically grown polypyrrole nanotubes by incorporating crosslinked glucose oxidase

Palod, Pragya Agar; Singh, Vipul

doi:10.1016/j.msec.2015.05.038

Cited by 35 publications

(18 citation statements)

References 54 publications

(62 reference statements)

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“…The enzyme used for the detection of glucose is glucose oxidase (GOx)2324252627. GOx catalyses the oxidation of glucose into gluconolactone and hydrogen peroxide28.…”

mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

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A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

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“…The enzyme used for the detection of glucose is glucose oxidase (GOx)2324252627. GOx catalyses the oxidation of glucose into gluconolactone and hydrogen peroxide28.…”

mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

View full text Add to dashboard Cite

show abstract

“…Therefore GO x solution when excited at 280 nm gives fluorescence emission at 330 nm. Emission intensity at 330 nm is proportional to the amount of GO x present in a solution [2]. This property of GO x has been well utilized for determining amount of immobilized enzymes over support matrix i.e.…”

Section: Synthesis Of Znr Enzyme Immobilization and Quantificationmentioning

confidence: 99%

“…Enzyme loading was measured to be 425 µg-cm -2 and 575 µg-cm -2 for physical adsorption and cross linking immobilization method respectively. Physical adsorption utilizes electrostatic attraction between GO x molecules (IEP=4.5) and ZnO (IEP=9.5) at neutral pH value while in case of cross linking, enzymes join in 3D structure by covalent bond formation between enzyme (GO x ) and bi-functional cross linking agent (Glutaraldehyde) [2]. Thus, cross linking has resulted in higher enzyme loading as compared to physical adsorption.…”

Section: Enzyme Quantificationmentioning

confidence: 99%

“…The output signal of such events is proportional to the analyte concentration [1]. Among the different categories of biosensors, enzymatic glucose biosensor has attracted interest of researchers due to its importance in diabetes care, beverage and food industry, drug discovery and analysis [2]. The body cells utilize glucose both as a source of energy and as a metabolic intermediate in the synthesis of more complex molecules such as fat [3].…”

Section: Introductionmentioning

confidence: 99%

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Effect of immobilization technique on performance ZnO nanorods based enzymatic electrochemical glucose biosensor

Shukla¹,

Pramila²,

Palani³

et al. 2017

J. Phys.: Conf. Ser.

Self Cite

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Abstract:In this paper, ZnO Nanorods (ZNR) have been synthesized over Platinum (Pt) coated glass substrate with in-situ addition KMnO 4 during hydrothermal growth process. Significant variation in ZnO nanostructures was observed by KMnO 4 addition during the growth. Glucose oxidase was later immobilized over ZNRs. The asprepared ZNRs were further utilized for glucose detection by employing amperometric electrochemical transduction method. In order to optimize the performance of the prepared biosensor two different immobilization techniques i.e. physical adsorption and cross linking have been employed and compared. Further investigations suggest that immobilization via cross linking method resulted in the improvement of the biosensor performance, thereby significantly affecting the sensitivity and linear range of the fabricated biosensor. Among the two types of biosensors fabricated using ZNR, the best performance was shown by cross linked electrodes. The sensitivity for the same was found to be 17.7 mA-cm -2 -M -1 , along with a wide linear range of 0.5-8.5 mM.

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“…On the other hand, metal and carbon nanomaterials are employed for constructing non-enzymatic glucose sensors [39]. Organic materials such as conducting polymers and phenylboronic acids are also used for developing enzyme-free glucose sensors [40,41,42,43]. In this context, early studies demonstrated the utility of Con A in constructing optical and electrochemical glucose sensors [44,45,46,47,48,49,50,51].…”

Section: Glucose Sensorsmentioning

confidence: 99%

Recent Progress in Lectin-Based Biosensors

Wang

Anzai

2015

Materials

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This article reviews recent progress in the development of lectin-based biosensors used for the determination of glucose, pathogenic bacteria and toxins, cancer cells, and lectins. Lectin proteins have been widely used for the construction of optical and electrochemical biosensors by exploiting the specific binding affinity to carbohydrates. Among lectin proteins, concanavalin A (Con A) is most frequently used for this purpose as glucose- and mannose-selective lectin. Con A is useful for immobilizing enzymes including glucose oxidase (GOx) and horseradish peroxidase (HRP) on the surface of a solid support to construct glucose and hydrogen peroxide sensors, because these enzymes are covered with intrinsic hydrocarbon chains. Con A-modified electrodes can be used as biosensors sensitive to glucose, cancer cells, and pathogenic bacteria covered with hydrocarbon chains. The target substrates are selectively adsorbed to the surface of Con A-modified electrodes through strong affinity of Con A to hydrocarbon chains. A recent topic in the development of lectin-based biosensors is a successful use of nanomaterials, such as metal nanoparticles and carbon nanotubes, for amplifying output signals of the sensors. In addition, lectin-based biosensors are useful for studying glycan expression on living cells.

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Improvement in glucose biosensing response of electrochemically grown polypyrrole nanotubes by incorporating crosslinked glucose oxidase

Cited by 35 publications

References 54 publications

Novel amperometric glucose biosensor based on MXene nanocomposite

Novel amperometric glucose biosensor based on MXene nanocomposite

Effect of immobilization technique on performance ZnO nanorods based enzymatic electrochemical glucose biosensor

Recent Progress in Lectin-Based Biosensors

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