The bake hardening response of a twin roll cast Al-Mg-Si (aluminium alloy AA6016) sheet, submitted to pre-aging treatments at 180uC shortly after solutionising, has been investigated by means of tensile testing, microhardness and electrical conductivity measurement, differential scanning calorimetry and transmission electron microscopy. During pre-aging at 180uC, stable Guinier-Preston type 1 (GP1) zones form first and suppress any subsequent natural aging. Clusters and small GP1 zones are reverted, and stable b0 nuclei are formed. These nuclei grow readily to become coherent b0 particles during the paint bake cycle, giving a considerable increase in strength. A pre-aging treatment for 2-10 min at 180uC offers a sufficient bake hardening response for the twin roll cast 6016 alloy sheet.