Improved β-carotene production by oxidative stress in Blakeslea trispora induced by liquid paraffin

Hu, Xianmei; Ma, Xiang; Tang, Pingwah; Yuan, Qipeng

doi:10.1007/s10529-012-1102-5

Cited by 13 publications

(4 citation statements)

References 12 publications

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“…Similarly, mated cultures of B. trispora contain lower superoxide dismutase (SOD) and catalase activities than a single culture, and addition of SOD inhibitors result in increased β-carotene content [ 261 ]. A similar result was obtained inducing the oxidative stress by paraffi n addition [ 262 ]. Despite these results, different data point to a lower effi ciency of β-carotene as a protective agent compared to xanthophylls.…”

Section: Biological Roles Of Fungal Carotenoidssupporting

confidence: 71%

Carotenoids

et al. 2014

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Section: Biological Roles Of Fungal Carotenoidssupporting

confidence: 71%

Carotenoids

et al. 2014

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“…Reactive oxygen species levels and oxidative stress are essential physiological phenomena in filamentous fungi and other microorganisms ( Macarisin et al, 2010 ; Nanou et al, 2011 ). On the one hand, moderate oxidative stress in the cell is an essential prerequisite for the growth and physiological metabolism of aerobic microbes, including the biosynthesis of the cell wall, proliferation, differentiation, and the formation of secondary metabolites ( Reverberi et al, 2010 ; Hu et al, 2011 ; Reverberi et al, 2012 ; Hu et al, 2013 ). Similarly, the increase of ROS levels in Aspergillus by adding cumene hydrogen peroxide (the peak of LOOH reached 140 μg/25 mL) promoted the formation of aflatoxin B 1 ( Reverberi et al, 2006 ).…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Pneumocandin B0 Production in Glarea lozoyensis by Low-Temperature Adaptive Laboratory Evolution

Song

Zhang

et al. 2018

Front. Microbiol.

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The production of pneumocandin B0 is limited by feedback inhibition. Here, low-temperature adaptive laboratory evolution (ALE) was used to improve the production capacity of Glarea lozoyensis by enhancing its membrane permeability. After 50 cycles of ALE, the pneumocandin B0 production of the endpoint strain (ALE50) reached 2131 g/L, which was 32% higher than the starting strain (ALE0). ALE50 showed a changed fatty acid composition of the cell membrane, which-+h increased its permeability by 14%, which in turn increased the secretion ratio threefold. Furthermore, ALE50 showed increased intracellular proline and acetyl-CoA concentrations, superoxide dismutase (SOD), and catalase (CAT) activity, as well as total antioxidant capacity. The slight biomass decrease in ALE50 was accompanied by decreased isocitrate dehydrogenase (ICDH) and glucose-6-phosphate dehydrogenase (G6PDH) activity. Finally, a putative model of the accumulation and secretion of pneumocandin B0 in ALE50 was established. ALE is a promising method to release intracellular feedback inhibition.

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“…Blakeslea trispora and Rhodotorula glutinis are the best-explored natural producers for β-carotene. The improvement in biosynthetic ability of these two strains have stemmed from the optimization of cultivation equipment 133 and the culture medium (Chang-he et al 2020 ; Tkáčová et al 2017 ), random mutagenesis and addition of exogenous stimulants (Hu et al 2013 ; Jing et al 2016 ; Luo et al 2021 ). The incomplete host genetic information and the lack of tools for genetic-level operation have thus far prevented more precise rational engineering of these strains.…”

Section: Major Natural Pigments and Advances In Biotechnological Prod...mentioning

confidence: 99%

Biotechnological advances for improving natural pigment production: a state-of-the-art review

Lyu

et al. 2022

Bioresour. Bioprocess.

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In current years, natural pigments are facing a fast-growing global market due to the increase of people’s awareness of health and the discovery of novel pharmacological effects of various natural pigments, e.g., carotenoids, flavonoids, and curcuminoids. However, the traditional production approaches are source-dependent and generally subject to the low contents of target pigment compounds. In order to scale-up industrial production, many efforts have been devoted to increasing pigment production from natural producers, via development of both in vitro plant cell/tissue culture systems, as well as optimization of microbial cultivation approaches. Moreover, synthetic biology has opened the door for heterologous biosynthesis of pigments via design and re-construction of novel biological modules as well as biological systems in bio-platforms. In this review, the innovative methods and strategies for optimization and engineering of both native and heterologous producers of natural pigments are comprehensively summarized. Current progress in the production of several representative high-value natural pigments is also presented; and the remaining challenges and future perspectives are discussed. Graphical Abstract

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Improved β-carotene production by oxidative stress in Blakeslea trispora induced by liquid paraffin

Cited by 13 publications

References 12 publications

Carotenoids

Carotenoids

Enhancement of Pneumocandin B0 Production in Glarea lozoyensis by Low-Temperature Adaptive Laboratory Evolution

Biotechnological advances for improving natural pigment production: a state-of-the-art review

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