Improved xylitol production by the novel inhibitor-tolerant yeast
            <i>Candida tropicalis</i>
            K2

Singh, Anup K.; Pandey, Ajay Kumar; Kumar, Mohit; Paul, Tanushree; Gaur, Naseem A.

doi:10.1080/09593330.2022.2095227

Cited by 4 publications

(6 citation statements)

References 67 publications

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“…Therefore, inhibitor-tolerant strains with improved xylitol yield and productivity are desirable for industrial application. In our previous study, C. tropicalis K2 was selected due to its inhibitor-tolerant phenotypes and superior xylitol yield and productivity [ 15 ]. Therefore, we tested the 5-HMF, furfural and acetic acid tolerant phenotypes of C. tropicalis K2M and C. tropicalis K21D.…”

Section: Resultsmentioning

confidence: 99%

“…The strain, C. tropicalis K2, was isolated and characterized in our previous study [ 15 ] (Table 3 ). For fermentation studies, cells were grown in YEPX (yeast extract 10 g/L, peptone 20 g/L and xylose 20 g/L) broth.…”

Section: Methodsmentioning

confidence: 99%

“…Acid pre-treatment of corncob was performedas described earlier [ 15 ]. Briefly, 2% v/v sulphuric acid was added to the powdered biomass (100 g ) and autoclaved at 121 °C (60 min).…”

Section: Methodsmentioning

confidence: 99%

“…Xylitol yield (g/g) was measured by the ratio of xylitol (g/L) produced vs xylose (g/L) utilized. The maximum xylitol productivity (g/Lh) was calculated as reported earlier[ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we identified a robust Candida tropicalis K2 natural isolate exhibiting inhibitor tolerance and osmotolerance characteristics along with the production of 90 g/L xylitol during batch fermentation using glycerol as a co-substrate [ 15 ]. In this study, we further improved xylitol production by developing a derivative of C. tropicalis K2 with reduced XDH activity and implemented no co-substrate approach for production of xylitol.…”

Section: Introductionmentioning

confidence: 99%

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Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery

Singh,

Deeba,

Kumar

et al. 2023

Microb Cell Fact

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Background Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly and sustainable compared to the chemical method. In this study, random mutagenesis and genetic engineering approaches were employed to develop Candida tropicalis strains with reduced xylitol dehydrogenase (XDH) activity to eliminate co-substrate requirement for corn cob-based xylitol-ethanol biorefinery. Results The results suggest that when pure xylose (10% w/v) was fermented in bioreactor, the Ethyl methane sulfonate (EMS) mutated strain (C. tropicalis K2M) showed 9.2% and XYL2 heterozygous (XYL2/xyl2Δ::FRT) strain (C. tropicalis K21D) showed 16% improvement in xylitol production compared to parental strain (C. tropicalis K2). Furthermore, 1.5-fold improvement (88.62 g/L to 132 g/L) in xylitol production was achieved by C. tropicalis K21D after Response Surface Methodology (RSM) and one factor at a time (OFAT) applied for media component optimization. Finally, corncob hydrolysate was tested for xylitol production in biorefinery mode, which leads to the production of 32.6 g/L xylitol from hemicellulosic fraction, 32.0 g/L ethanol from cellulosic fraction and 13.0 g/L animal feed. Conclusions This work, for the first time, illustrates the potential of C. tropicalis K21D as a microbial cell factory for efficient production of xylitol and ethanol via an integrated biorefinery framework by utilising lignocellulosic biomass with minimum waste generation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Acid pre-treatment of corncob was performedas described earlier [ 15 ]. Briefly, 2% v/v sulphuric acid was added to the powdered biomass (100 g ) and autoclaved at 121 °C (60 min).…”

Section: Methodsmentioning

confidence: 99%

“…Xylitol yield (g/g) was measured by the ratio of xylitol (g/L) produced vs xylose (g/L) utilized. The maximum xylitol productivity (g/Lh) was calculated as reported earlier[ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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