Improved Understanding of Acoustophoresis and Development of an Acoustofluidic Device for Blood Plasma Separation

Karthick, S.; Sen, A. K.

doi:10.1103/physrevapplied.10.034037

Cited by 29 publications

(23 citation statements)

References 26 publications

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“…The design of the acoustics-based blood plasma separation module (Figs. 1a and S2a) is reported elsewhere 28 . The width of the main channel is 300 µm to support half-wave at a frequency of 1.91 MHz, as calculated from theory and confirmed in experiments.…”

Section: Methodsmentioning

confidence: 77%

“…A borosilicate glass slide (Semiconductor Technology and Applications, USA) of 500 µm thickness is then bonded with the DRIE-etched silicon wafer by anodic bonding to seal the channels. A detailed procedure followed for the fabrication of the blood plasma separation module is reported elsewhere 28 . Module II (mixing and incubation module) and Module III (optical detection module) are machined in Polymethyl methacrylate (PMMA) using a CNC micro-milling machine (Minitech machinery, USA) as per the design outlined in the previous section.…”

Section: Methodsmentioning

confidence: 99%

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Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Gaikwad

Thangaraj

Sen

2021

Sci Rep

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The levels of hydrogen peroxide ($${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 ) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in plasma in the concentration range of 0–49 µM, offering a limit of detection of 0.05 µM, a sensitivity of 0.60 µM−1, and detection time of 15 min; the device is amenable to the real-time measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in the patient’s blood. Using the linear correlation obtained with known quantities of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 , the endogenous $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 concentration in the blood of healthy individuals is found to be in the range of 0.8–6 µM. The availability of this device at the point of care will have relevance in understanding the role of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in health and disease.

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Section: Methodsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Gaikwad

Thangaraj

Sen

2021

Sci Rep

Self Cite

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“…An example of such an application is a silicon micro-channel, used to provide an improved blood plasma separation from whole blood by acoustophoresis. Karthick et al [30] harnessed the theory of acoustophoresis of dense suspension to understand the acoustic focusing of cells within blood capillaries. Such works are not only inspiring but also have promising applications.…”

Section: The Last Decade-an Age Of Great Promisementioning

confidence: 99%

Bioinspired NEMS—Prospective of Collaboration with Nature

et al. 2022

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“…Inertial microfluidics can be used to separate plasma from whole blood sample in a highthroughput way [7,8]. Blood separation can also be achieved on microfluidic devices using acoustophoresis [9] and dielectrophoretic force [10]. Household tools or toys including egg beaters [11], smartphones [12], and paper centrifuges [13] have been developed to separate plasma from blood.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Passive Plasma Separation on OSTE Pillar Forest

et al. 2021

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Plasma separation is of high interest for lateral flow tests using whole blood as sample liquids. Here, we built a passive microfluidic device for plasma separation with high performance. This device was made by blood filtration membrane and off-stoichiometry thiol–ene (OSTE) pillar forest. OSTE pillar forest was fabricated by double replica moldings of a laser-cut polymethylmethacrylate (PMMA) mold, which has a uniform microstructure. This device utilized a filtration membrane to separate plasma from whole blood samples and used hydrophilic OSTE pillar forest as the capillary pump to propel the plasma. The device can be used to separate blood plasma with high purity for later use in lateral flow tests. The device can process 45 μL of whole blood in 72 s and achieves a plasma separation yield as high as 60.0%. The protein recovery rate of separated plasma is 85.5%, which is on par with state-of-the-art technologies. This device can be further developed into lateral flow tests for biomarker detection in whole blood.

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Improved Understanding of Acoustophoresis and Development of an Acoustofluidic Device for Blood Plasma Separation

Cited by 29 publications

References 26 publications

Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

Bioinspired NEMS—Prospective of Collaboration with Nature

High-Performance Passive Plasma Separation on OSTE Pillar Forest

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