Improved Swelling Property of Tissue Adhesive Hydrogels Based on α‐Cyclodextrin/Decyl Group‐Modified Alaska Pollock Gelatin Inclusion Complexes

Abstract

Adhesives/sealants are used after suturing to prevent leakage of cerebrospinal fluid from an anastomotic site. Commercial adhesives/sealants have been used to close the cerebral dura. However, swelling of the cured adhesives/sealants induces increased intracranial pressure and decreases the strength of the seal. In the present study, tissue adhesive hydrogels with improved swelling property using inclusion complex composed of 𝜶-cyclodextrin (𝜶CD) and decyl group (C10)-modified Alaska pollock-derived gelatin (C10-ApGltn) with a high degree of substitution (DS) (>20 mol%) are developed. Viscosity of C10-ApGltn with a high DS solution remarkably decreased by the addition of 𝜶CD. The resulting 𝜶CD/C10-ApGltn adhesive hydrogel composed of 𝜶CD/C10-ApGltn inclusion complexes and poly(ethylene glycol) (PEG)-based crosslinker showed improved swelling property after immersion in saline. Also, the resulting adhesive has a significantly higher burst strength than fibrin-based adhesives and is as strong as a PEG-based adhesive. Quantitative analysis of 𝜶CD revealed that the improved swelling property of the resulting adhesive hydrogels is induced by the release of 𝜶CD from cured adhesive, and the subsequent assembly of decyl groups in the saline. These results suggest that adhesives developed using the 𝜶CD/C10-ApGltn inclusion complex can be useful for closing the cerebral dura mater.

A low-swelling hydrogel as a multirole sealant for efficient dural defect sealing and prevention of postoperative adhesion

“…Recently, efforts have been made on the development of hydrogel-based dural sealants. Notably, to reduce the swelling ratios of hydrogels, the stability of hydrogel networks was enhanced by introduction of hydrophobic interactions between hydrophobic side groups or thermo-induced micellar self-assembly [ 39 , 42 ]. In this work, the 4aPEG-OPA/gelatin hydrogel exhibited a low swelling ratio (33.3% ± 5.0%) and relatively high burst pressure (Fig.…”