Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue

Ratushnyak, M. G.; Semochkina, Yu. P.; Zhirnik, A. S.; Smirnova, O. D.

doi:10.1007/s10517-021-05368-0

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Ionizing radiation of the developing or adult brain is acknowledged as a potential cause of cognitive impairment and neurodegeneration, particularly when neural stem cells are affected [ 39 , 40 , 41 , 42 ]. Elucidating the mechanism underlying irradiation-mediated NSC injury would contribute to alleviating the side effects of radiotherapy and demystifying the induction of neural inflammation, brain development, and even neurodegeneration-associated mechanisms [ 43 , 44 , 45 ]. The substitution models employed in preclinical radiation research vary from cultured cells to small or large animals [ 46 , 47 ]; the majority of these models have been established according to the linear quadratic (LQ) model [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation

et al. 2022

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Cranial radiation therapy is one of the most effective treatments for childhood brain cancers. Despite the ameliorated survival rate of juvenile patients, radiation exposure-induced brain neurogenic region injury could markedly impair patients’ cognitive functions and even their quality of life. Determining the mechanism underlying neural stem cells (NSCs) response to irradiation stress is a crucial therapeutic strategy for cognitive impairment. The present study demonstrated that X-ray irradiation arrested NSCs’ cell cycle and impacted cell differentiation. To further characterize irradiation-induced molecular alterations in NSCs, two-dimensional high-resolution mass spectrometry-based quantitative proteomics analyses were conducted to explore the mechanism underlying ionizing radiation’s influence on stem cell differentiation. We observed that ionizing radiation suppressed intracellular protein transport, neuron projection development, etc., particularly in differentiated cells. Redox proteomics was performed for the quantification of cysteine thiol modifications in order to profile the oxidation-reduction status of proteins in stem cells that underwent ionizing radiation treatment. Via conjoint screening of protein expression abundance and redox status datasets, several significantly expressed and oxidized proteins were identified in differentiating NSCs subjected to X-ray irradiation. Among these proteins, succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (sdha) and the acyl carrier protein, mitochondrial (Ndufab1) were highly related to neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, and Huntington’s disease, illustrating the dual-character of NSCs in cell differentiation: following exposure to ionizing radiation, the normal differentiation of NSCs was compromised, and the upregulated oxidized proteins implied a degenerative differentiation trajectory. These findings could be integrated into research on neurodegenerative diseases and future preventive strategies.

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Section: Discussionmentioning

confidence: 99%

Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation

et al. 2022

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“…Additionally, the extracellular vesicles derived from MSCs during stroke exhibit a remarkable ability to selectively migrate toward sites of injury, thereby safeguarding neural tissues against additional necrotic expansion, promoting late‐stage vascularization, and releasing growth factors that facilitate tissue remodeling 49 . In the context of cotransplantation with other cell types, MSCs exhibit the capability to stimulate the differentiation of NSCs and augment the survival rates of transplanted NSCs 50,51 . These distinctive attributes position MSCs as a promising therapeutic modality for mitigating inflammation and addressing neurodegenerative alterations linked to various neuro‐related diseases across diverse clinical scenarios.…”

Section: Mechanisms Of Heterogeneous Cell Transplantation For Neurolo...mentioning

confidence: 99%

“… 49 In the context of cotransplantation with other cell types, MSCs exhibit the capability to stimulate the differentiation of NSCs and augment the survival rates of transplanted NSCs. 50 , 51 These distinctive attributes position MSCs as a promising therapeutic modality for mitigating inflammation and addressing neurodegenerative alterations linked to various neuro‐related diseases across diverse clinical scenarios.…”

Section: Mechanisms Of Heterogeneous Cell Transplantation For Neurolo...mentioning

confidence: 99%

Role and limitation of cell therapy in treating neurological diseases

Li,

Tao

et al. 2024

Ibrain

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The central role of the brain in governing systemic functions within human physiology underscores its paramount significance as the focal point of physiological regulation. The brain, a highly sophisticated organ, orchestrates a diverse array of physiological processes encompassing motor control, sensory perception, cognition, emotion, and the regulation of vital functions, such as heartbeat, respiration, and hormonal equilibrium. A notable attribute of neurological diseases manifests as the depletion of neurons and the occurrence of tissue necrosis subsequent to injury. The transplantation of neural stem cells (NSCs) into the brain exhibits the potential for the replacement of lost neurons and the reconstruction of neural circuits. Furthermore, the transplantation of other types of cells in alternative locations can secrete nutritional factors that indirectly contribute to the restoration of nervous system equilibrium and the mitigation of neural inflammation. This review summarized a comprehensive investigation into the role of NSCs, hematopoietic stem cells, mesenchymal stem cells, and support cells like astrocytes and microglia in alleviating neurological deficits after cell infusion. Moreover, a thorough assessment was undertaken to discuss extant constraints in cellular transplantation therapies, concurrently delineating indispensable model‐based methodologies, specifically on organoids, which were essential for guiding prospective research initiatives in this specialized field.

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Stem Cell Exosomes Improve Survival of Neural Stem Cells after Radiation Exposure

Ratushnyak

Semochkina²,

Yastremsky³

et al. 2022

Bull Exp Biol Med

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Improved Survival and Regeneration of Irradiated Mouse Neural Stem Cells after Co-Culturing with Non-Irradiated Mouse Neural Stem Cells or Mesenchymal Stem Cells from the Adipose Tissue

Cited by 4 publications

References 27 publications

Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation

Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation

Role and limitation of cell therapy in treating neurological diseases

Stem Cell Exosomes Improve Survival of Neural Stem Cells after Radiation Exposure

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