Improved Stress Tolerance of Saccharomyces cerevisiae by CRISPR-Cas-Mediated Genome Evolution

Mitsui, Ryosuke; Yamada, R.; Ogino, Hiroyasu

doi:10.1007/s12010-019-03040-y

Cited by 29 publications

(22 citation statements)

References 30 publications

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“…CRISPR/Cas9 system also amplified the power of evolutionary engineering for industrial microorganisms. Mitsui et al developed CRISPR/Cas9 system as a genome shuffling method for evolutionary engineering to obtain a thermotolerant mutant strain ( Mitsui et al, 2019 ). Halperin et al (2018) proposed a new method called EvolvR that can accelerate mutagenesis up to 7,770,000-fold within a tunable window length via CRISPR-guided nickases.…”

Section: Application Of Crispr/cas System In Microbial Biotechnologymentioning

confidence: 99%

Development and Application of CRISPR/Cas in Microbial Biotechnology

Ding

Yang

Shi

2020

Front. Bioeng. Biotechnol.

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The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods for applications in microbial biotechnology. Here, key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA. Following an overview of various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing, we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening. We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds, and end with perspectives of future advancements.

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Section: Application Of Crispr/cas System In Microbial Biotechnologymentioning

confidence: 99%

Development and Application of CRISPR/Cas in Microbial Biotechnology

Ding

Yang

Shi

2020

Front. Bioeng. Biotechnol.

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“…In Cunha et al [103], two pathways (XR/XDH or XI) of xylose assimilation by S. cerevisiae were compared in ethanol production under different fermentation conditions, demonstrating satisfactory results for the feasibility of this fuel from non-detoxified hemicellulosic hydrolysates. Meanwhile, Mitsui et al [104] developed a novel genome shuffling method using CRISPR-Cas to improve stress tolerance in S. cerevisiae yeast. Regarding E. coli, its main disadvantages refer to the narrow growth range of neutral pH (6.0-8.0), in addition to ethanol not being a core product for this bacterium.…”

Section: Perspectives For Future Research and Innovationsmentioning

confidence: 99%

Technological Advances in Synthetic Biology for Cellulosic Ethanol Production

Fantinel¹,

Margis²,

Talamini³

et al. 2022

Biorefineries - Selected Processes

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The resurgence of biofuels in the recent past has brought new perspectives for renewable energy sources. Gradually the optimistic scenarios were being challenged by the competition for raw materials dedicated to direct or indirect human food. Second-generation biorefineries have emerged as technological alternatives to produce biofuels from lignocellulosic biomass. The third generation of biorefineries uses alternative raw materials like algae and microalgae. Despite the technical feasibility, these biorefineries were indebted for their economic performance. Synthetic biology has provided new microbial platforms that are increasingly better adapted to industrial characteristics to produce biofuels and fine chemicals. Synthetic biology bioengineers microorganisms to take advantage of the low-cost and less-noble raw materials like lignocellulosic biomass, carbon dioxide, and waste as a sustainable alternative for bioenergy generation using bio-substrates. In this chapter, we analyze the innovations in synthetic biology as applied to cellulosic ethanol production based on registered patents issued over the last twenty years (1999–2019). Using Questel-Orbit Intelligence, we recovered a total of 298 patent families, from which we extracted the key concepts and technology clusters, the primary technological domains and applications, the geographical distribution of patents, and the leading patents assignees. Besides, we discuss the perspectives for future research and innovations and the market and policy opportunities for innovation in this technological field. We conclude that the patented technologies serve as a proxy for the development of synthetic biotechnology applied in cellulosic ethanol production by the fourth generation of biorefineries.

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“…Since environmental stress tolerance mechanism is still obscure, the majority of the methods depend on random mutagenesis, such as adaptive laboratory evolution [115], UV mutagenesis [116] (Table 3). A new genome shuffling technique with CRISPR system was used to realize environmental stress tolerance by genome evolution under environmental stress conditions [117]. Increasing the ploidy of yeast is also an option for researchers to improve yeast tolerance [118].…”

Section: How To Improve the Environmental Stress Tolerance Of Yeasts?mentioning

confidence: 99%

Overview of yeast environmental stress response pathways and the development of tolerant yeasts

Lin

2021

Syst Microbiol and Biomanuf

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Yeast is widely used for industrial production of various types of products, such as ethanol and enzymes. However, its fermentation efficiency is strongly reduced by harmful environmental stresses. Specifically, harmful environmental stresses damage important cellular components, such as cell wall, cell membrane, proteins, etc. Then, these damages cause cellular metabolic disorders or even death. In the past decades, there has been a portfolio of studies on the environmental stress tolerance of yeasts, which mainly aimed at cell damages caused by different environmental stresses, different ways to improve yeast environmental stress tolerance or a tolerance mechanism for certain environmental stress. However, a comprehensive overview of how yeasts respond to environmental stresses is lacking, and the correlation of tolerance mechanism between different environmental stresses is unclear. In this review, we summarized the general damages induced by most of environmental stresses, the existing major mechanisms of environmental stress tolerance from the perspective of key signalling pathways, and the common ways to improve the resistance to environmental stresses in yeast cells. The tolerance mechanisms of yeast cells to different environmental stresses are diverse, but sometimes they share the same signalling pathway. Cells use sensors on the cell surface to recognize environmental stresses and transmit signals to the nucleus to cause changes in gene expression. By summarizing the main signalling pathways, including MAPK pathway, cAMP/PKA pathway, YAP1/ SKN7 pathway, it will provide a powerful reference for future efforts to promote yeast environmental stress tolerance and study yeast tolerance mechanisms.

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Improved Stress Tolerance of Saccharomyces cerevisiae by CRISPR-Cas-Mediated Genome Evolution

Cited by 29 publications

References 30 publications

Development and Application of CRISPR/Cas in Microbial Biotechnology

Development and Application of CRISPR/Cas in Microbial Biotechnology

Technological Advances in Synthetic Biology for Cellulosic Ethanol Production

Overview of yeast environmental stress response pathways and the development of tolerant yeasts

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