Improved single-cell culture achieved using micromolding in capillaries technology coupled with poly (HEMA)

Ye, Fang; Jiang, Jin; Chang, Honglong; Xie, Li; Deng, Jinjun; Ma, Zhibo; Yuan, Weizheng

doi:10.1063/1.4926807

Cited by 11 publications

(15 citation statements)

References 22 publications

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“…Characterization of the p‐HEMA surface demonstrates that the p‐HEMA coating achieves a thickness of 0.3 µm. In comparison, previous methods for fabrication of p‐HEMA micromolded surfaces result in 5 µm thick layers of p‐HEMA . Measurement of the p‐HEMA surface via SEM indicates that p‐HEMA etching results in patterned p‐HEMA, which adheres closely to the geometry of the microfluidic channel (Figure ).…”

Section: Resultsmentioning

confidence: 90%

“…In comparison, previous methods for fabrication of p-HEMA micromolded surfaces result in 5 mm thick layers of p-HEMA. 13,14 Measurement of the p-HEMA surface via SEM indicates that p-HEMA etching results in patterned p-HEMA, which adheres closely to the geometry of the microfluidic channel ( Figure 2). Swelling of the patterned p-HEMA coating was then assessed.…”

Section: P-hema Etchingmentioning

confidence: 77%

“…Viability of C2C12 myoblasts after patterning was not directly examined in this manuscript; however, the high number of adherent cells after 7 DIV suggests that p‐HEMA patterning does not have adverse effects on cell viability (Figure ). In addition, other work from multiple groups employed p‐HEMA specifically for its biocompatibility with multiple cellular systems …”

Section: Resultsmentioning

confidence: 99%

“…Historically, poly(2‐hydroxyethyl methacrylate) (p‐HEMA) has been used to create regions of decreased protein and cell adhesion of spheroid cultures, and in specific, rat hepatocytes . The fabrication of p‐HEMA‐coated regions with microfluidic devices has been used for creation of walls and molds that allow for single cell isolation of cells and other cell types . While very useful for studies of single cells, this fabrication type has several limitations.…”

Section: Introductionmentioning

confidence: 99%

“…[10][11][12] The fabrication of p-HEMA-coated regions with microfluidic devices has been used for creation of walls and Correspondence concerning this article should be addressed to Bonnie L. Firestein at Firestein@biology.rutgers.edu molds that allow for single cell isolation of cells and other cell types. 13,14 While very useful for studies of single cells, this fabrication type has several limitations. For example, the ability to fabricate specific areas for cell adhesion and growth is limited by the use of closed channels.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic device‐assisted etching of p‐HEMA for cell or protein patterning

Kung

Sillitti

Shreiber

et al. 2017

Biotechnology Progress

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The construction of biomaterials with which to limit the growth of cells or to limit the adsorption of proteins is essential for understanding biological phenomena. Here, we describe a novel method to simply and easily create thin layers of poly (2-hydroxyethyl methacrylate) (p-HEMA) for protein and cellular patterning via etching with ethanol and microfluidic devices. First, a cell culture surface or glass coverslip is coated with p-HEMA. Next, a polydimethylsiloxane (PDMS) microfluidic is placed onto the p-HEMA surface, and ethanol is aspirated through the device. The PDMS device is removed, and the p-HEMA surface is ready for protein adsorption or cell plating. This method allows for the fabrication of 0.3 µm thin layers of p-HEMA, which can be etched to 10 µm wide channels. Furthermore, it creates regions of differential protein adhesion, as shown by Coomassie staining and fluorescent labeling, and cell adhesion, as demonstrated by C2C12 myoblast growth. This method is simple, versatile, and allows biologists and bioengineers to manipulate regions for cell culture adhesion and growth. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:243-248, 2018.

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Section: Resultsmentioning

confidence: 90%

Section: P-hema Etchingmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microfluidic device‐assisted etching of p‐HEMA for cell or protein patterning

Kung

Sillitti

Shreiber

et al. 2017

Biotechnology Progress

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Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

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In recent years, the use of microfabrication techniques has allowed biomaterials studies which were originally carried out at larger length scales to be miniaturized as so-called "on-chip" experiments. These miniaturized experiments have a range of advantages which have led to an increase in their popularity. A range of biomaterial shapes and compositions are synthesized or manufactured on chip. Moreover, chips are developed to investigate specific aspects of interactions between biomaterials and biological systems. Finally, biomaterials are used in microfabricated devices to replicate the physiological microenvironment in studies using so-called "organ-on-chip," "tissue-on-chip" or "disease-on-chip" models, which can reduce the use of animal models with their inherent high cost and ethical issues, and due to the possible use of human cells can increase the translation of research from lab to clinic. This review gives an overview of recent developments at the interface between microfabrication and biomaterials science, and indicates potential future directions that the field may take. In particular, a trend toward increased scale and automation is apparent, allowing both industrial production of micron-scale biomaterials and high-throughput screening of the interaction of diverse materials libraries with cells and bioengineered tissues and organs.

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Regulation of cell arrangement using a novel composite micropattern

Liu

Zhang

et al. 2017

J Biomedical Materials Res

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Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single-cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two-cell and three-cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi-cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093-3101, 2017.

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Improved single-cell culture achieved using micromolding in capillaries technology coupled with poly (HEMA)

Cited by 11 publications

References 22 publications

Microfluidic device‐assisted etching of p‐HEMA for cell or protein patterning

Microfluidic device‐assisted etching of p‐HEMA for cell or protein patterning

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Regulation of cell arrangement using a novel composite micropattern

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