Improved simultaneous determination method of β-carotene and retinol with saponification in human serum and rat liver

Hosotani, Keisuke; Kitagawa, Maki

doi:10.1016/s1570-0232(03)00233-2

Cited by 42 publications

(44 citation statements)

References 26 publications

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“…The extraction of β-carotene and retinol from the liver of each animal was carried out by the method described previously (Hosotani and Kitagawa, 2003) with some modifications. This method is highly specific and yeilds higher concentrations of both β-carotene and retinol.…”

Section: Extraction and Hplc Analysis Of β-Carotene And Retinolmentioning

confidence: 99%

Carotenoids as regulators for inter-species difference in cytochrome P450 1A expression and activity in ungulates and rats

Darwish

Ikenaka

Ohno

et al. 2010

Food and Chemical Toxicology

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3 AbstractUngulates (deer, cattle, and horses) are reported as animal species which show extremeaccelerated metabolism of CYP1A substrates, such as ethoxyresorufin compared to rats. This study was undertaken to investigate whether accumulation of carotenoids is a possible cause for inter-species difference in CYP1A-dependent activity in this group of animals. The relationship between inter-species differences in CYP1A-dependent activity and the accumulated carotenoids and retinoids as candidates of dietary CYP1A inducers in ungulate species was clarified. Interestingly, there were positive correlations between the accumulated carotenoids, such as β-carotene, with both EROD activity and CYP1A protein expression. These correlations were negative with the accumulated retinoids, such as retinol. The β-carotene was major component of carotenoids in ungulates, and known as an inducer of CYP1A. On the other hand, the retinol is reported as the reducer of CYP1A. Other factors which affect CYP1A1 expression, such as polycyclic aromatic hydrocarbons, were also analyzed. To cancel the effects of interspecies difference in CYP1A induction signal cascade among these animals, the rat cell line (H4-II-cells) was treated with the extracted carotenoids from the examined animals. In conclusion, carotenoids and retinoids may have direct effects on the inter-species differences in CYP1A-dependent activity and protein expression.4

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Section: Extraction and Hplc Analysis Of β-Carotene And Retinolmentioning

confidence: 99%

Carotenoids as regulators for inter-species difference in cytochrome P450 1A expression and activity in ungulates and rats

Darwish

Ikenaka

Ohno

et al. 2010

Food and Chemical Toxicology

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“…However, no cisisomers were detected. Hosotani and Kitagawa [25] employed a C-18 column with a quaternary mobile phase of acetonitrile/dichloromethane/methanol/1-octanol (90:15:10:0.1, v/v/v/v) for separation of only ␤-carotene in human serum. An HPLC method was developed by Lee et al [26], who used a C-18 column and an isocratic system with a mixture of acetonitrile and methanol (65:35, v/v) for separation of ␤-cryptoxanthin, ␣-carotene, ␤-carotene, lutein, zeaxanthin, lycopene and canthaxanthin in human serum.…”

Section: Hplc Separation Of Carotenoids In Human Serummentioning

confidence: 99%

An improved HPLC method for determination of carotenoids in human serum

Rajendran

Chen

2005

Journal of Chromatography B

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“…Plasma and liver retinol analysis: Plasma and liver retinol was analyzed by HPLC according to Hosotani and Kitagawa (2003). Retinol extraction from plasma was carried out by mixing 0.15 mL of plasma with 0.35 mL water, 0.5 mL 3% sodium ascorbate and 2 mL ethanol containing 0.135% BHT.…”

Section: Methodsmentioning

confidence: 99%

Bioconversion of Carotenoids in Five Fruits and Vegetables to Vitamin A Measured by Retinol Accumulation in Rat Livers

Carrillo-Lopez¹

2010

American Journal of Agricultural and Biological Sciences

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Problem statement: Vitamin A deficiency is one of the most prevalent and major nutritional problems in developing countries, especially in young children. In many countries, a substantial proportion of dietary vitamin A is commonly derived from pro-vitamin A carotenoids obtained from colored fruits and orange or green vegetables. However, the bioavailability of retinol derived from carotenoids from these plant sources is not well known. Approach: The present study analyzed β-Carotene and Total Carotenoids (TC) composition of carrots (Daucus carota), parsley (Petroselinum crispum), Spinach (Spinacea oleracea), mangoes (Mangifera indica) and papayas (Carica papaya) and determined the bioconversion of their carotenoids to vitamin A by monitoring the levels of retinol accumulated in liver and plasma of Wistar rats (Rattus norvegicus). Products were freeze-dried, β-Carotene content analyzed by HPLC and TC by Spectrophotometry. Results: Carrots presented the highest content of β-carotene followed by parsley with 32.8 and 19.6 mg 100 g −1 , respectively. Spinach had the highest content of TC followed by parsley with 60.7 and 56.7 mg 100 g −1 , respectively. Four-week-old male Wistar rats received a standard diet as an adaptation period, a diet free of Carotenoids and Vitamin A (CVA-diet) as depletion period and finally a Fruit or Vegetable (FoV) based diet as repletion period. The highest β-carotene bioconversion was for mango and the lowest for parsley, whereas the highest TC bioconversion was for carrots and the lowest for parsley. There were no significant differences in plasma retinol between treatments. Conclusion/Recommendations: There was no relation between carotenoids content in FoV-based diet and retinol status in plasma. Furthermore, the employment of a general retinol conversion factor is regarded as not appropriate. So, it is recommended to consider specific conversion factors for groups of horticultural crops, for example, a factor for green leafy vegetables and other factor for fruits or roots.

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Improved simultaneous determination method of β-carotene and retinol with saponification in human serum and rat liver

Cited by 42 publications

References 26 publications

Carotenoids as regulators for inter-species difference in cytochrome P450 1A expression and activity in ungulates and rats

Carotenoids as regulators for inter-species difference in cytochrome P450 1A expression and activity in ungulates and rats

An improved HPLC method for determination of carotenoids in human serum

Bioconversion of Carotenoids in Five Fruits and Vegetables to Vitamin A Measured by Retinol Accumulation in Rat Livers

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