Improved serodetection of Mycobacterium bovis infection in badgers (Meles meles) using multiantigen test formats

Greenwald, Rena; Esfandiari, Javan; Lesellier, Sandrine; Houghton, Raymond L.; Pollock, J.M.; Aagaard, Claus; Andersen, Peter; Hewinson, R. Glyn; Chambers, Mark A.; Lyashchenko, Konstantin P.

doi:10.1016/s0732-8893(03)00046-4

Cited by 90 publications

(93 citation statements)

References 25 publications

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“…Table 2 shows the frequencies of IgG reactivity to M. bovis antigens found in infected and control animals. The MAPIA results confirmed our previous observations that MPB83 protein is serodominant in badgers with M. bovis infection and in experimentally infected white-tailed deer (Greenwald et al, 2003;Waters et al, 2004). The present study further revealed that this molecule is the most reactive antigen in brushtail possums and wild boar.…”

Section: Antibody Responses and Antigen Recognitionsupporting

confidence: 91%

“…A simple and rapid antibody detection assay was developed by Chembio Diagnostic Systems, Inc. using colored latex-based lateral-flow technology and a cocktail of selected M. bovis antigens including ESAT-6, CFP10, and MPB83 (Greenwald et al, 2003;Waters et al, 2006). Serum specimens were tested for the presence of specific antibody as previously described .…”

Section: Rapid Test (Rt)mentioning

confidence: 99%

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Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Lyashchenko¹,

Greenwald²,

Esfandiari³

et al. 2008

Veterinary Microbiology

116

108

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Lyashchenko, K.P.; Greenwald, R.; Esfandiari, J.; Chambers, M.A.; Vicente, J.; Gortazar, C.; Santos, N.; Correia-Neves, M.; Buddle, B.M.; Jackson, R.; O'Brien, D.J.; Schmitt, S.; Palmer, M. V.; Delahay, R.J.; and Waters, W.R., "Animal-side serologic assay for rapid detection of Mycobacterium Bovis infection in multiple species of free-ranging wildlife" (2008 AbstractNumerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n = 1532), white-tailed deer (n = 463), brushtail possums (n = 129), and wild boar (n = 177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT This article is a U.S. government work, and is not subject to copyright in the United States.may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock. #

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Section: Antibody Responses and Antigen Recognitionsupporting

confidence: 91%

Section: Rapid Test (Rt)mentioning

confidence: 99%

Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Lyashchenko¹,

Greenwald²,

Esfandiari³

et al. 2008

Veterinary Microbiology

116

108

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“…Drewe, unpublished observations) or closely related bacteria resulting in a cross-reactive response. 23,42 Seroreactivity of badgers to antigens of environmental mycobacteria was found to be widespread in one study, 40 and it is feasible that a similar process occurs in meerkats. Antibodies to 7 antigens of the M. tuberculosiscomplex (MPB83, 38 kDa protein, ESAT-6, CFP10, MPB70, Acr1, and fusion protein CFP10/ESAT-6) were detected only in meerkats culturing positive for M. bovis (Table 1) suggesting that a serologic test examining for antibodies to only these proteins may be of higher specificity than the current test based on a wider range of antibody responses.…”

Section: Discussionmentioning

confidence: 97%

“…This was because studies using MAPIA in European badgers (Meles meles) suggest low specificity of this band, which is likely to be due to components of MBCF being common to environmental mycobacteria that sensitize badgers and are responsible for cross-recognition of MBCF. 23 Case definition. To elucidate the relative reactivity to different MAPIA antigens of meerkats with and without bTB, MAPIA results were compared with results from mycobacterial culture of pooled tissues (lung, spleen, and lymph nodes) collected subsequently from the same individual animals during detailed postmortem examination.…”

Section: Sample Collectionmentioning

confidence: 99%

Accuracy of Three Diagnostic Tests for Determining Mycobacterium Bovis Infection Status in Live-Sampled Wild Meerkats (Suricata Suricatta)

Drewe¹,

Dean

Michel

et al. 2009

J VET Diagn Invest

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Abstract. Accurate diagnosis of Mycobacterium bovis infection (bovine tuberculosis [bTB]) in live animals is notoriously problematic. The aim of the present study was to evaluate the diagnostic potential of 2 new serologic tests (multiantigen print immunoassay [MAPIA] and lateral flow immunoassay rapid test [RT]) in comparison with mycobacterial culture of tracheal washes for determining M. bovis infection status in a freeranging population of wild meerkats (Suricata suricatta). During a longitudinal study lasting 2.5 years, 240 individually identifiable meerkats were each sampled up to 8 times under anesthesia every 3 months. Diagnostic accuracy was determined through Bayesian and maximum likelihood estimations of sensitivity, specificity, and likelihood ratios for each diagnostic test when used independently and in parallel to classify the disease status of individual meerkats in the absence of a gold standard. Culture of tracheal washes was highly specific (0.99; 95% confidence interval [CI] 5 0.77, 1.00) but of low sensitivity (0.36; 95% CI 5 0.24, 0.50) for diagnosing M. bovis-infected individuals. The longitudinal nature of the study with repeated sampling of the same individual animals served simultaneously to improve chances of detecting infection and increase confidence in a negative result in individual animals repeatedly testing negative. Although MAPIA and RT were individually of limited diagnostic use, interpreting the results of these 2 tests in parallel produced estimates of sensitivity (0.83; 95% CI 5 0.67, 0.93) and specificity (0.73; 95% CI 5 0.62, 0.82) high enough to usefully inform decision making when determining exposure to bTB in wild meerkats and potentially other species in which bTB poses a diagnostic challenge.

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“…Recent studies have proven the rapid test to be useful in detecting M. bovis infections in llamas (Llama glama), elk (Cervus elaphus), white-tailed deer (Odocoileus virginianus), wild boar (Sus scrofa), Eurasian badgers, brushtail possums (Trichosurus vulpecula), and meerkats (Suricata suricatta); however, validation of the rapid test for domestic cats has yet to be achieved. 6,9,10,12,16,17,26 Multiple antigen print immunoassay identified 16/83, 16 kDa, MPB83, and MBCF as major antigens involved in the humoral immune response in sensitinogen-stimulated cats. Visually all sensitinogen-stimulated cats reacted consistently to antigen MBCF and less consistently to 16/ 83, 16 kDa, and MPB83 antigens (Fig.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Three Immunodiagnostic Assays for Antemortem Detection of Mycobacterium Bovis Stimulation in Domestic Cats

et al. 2010

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Abstract. Mycobacterium bovis causes disease in numerous mammalian species including humans, thus making research, surveillance, and control important in the eradication of tuberculosis. Domestic cats are susceptible to multiple mycobacterial species including Mycobacterium bovis; however, their role in the epidemiology of bovine tuberculosis is not fully documented. The current study was an evaluation of the immune response in specific pathogen-free (SPF) cats stimulated with sensitinogen, a heat-killed M. bovis product, using the rapid test, multiple antigen print immunoassay (MAPIA), and bovine-purified protein derivative (bPPD) single skin test. Six cats were inoculated with sensitinogen subcutaneously on days 0 and 24; 2 noninoculated cats and 49 non-SPF cats were controls. Serial serum samples were collected during 135 days and assayed for M. bovis antibodies by rapid test and MAPIA. On day 123, bPPD skin test was performed and read at 48 and 72 hr. The bPPD test at 72 hr had a mean skin thickness of 0.3 mm for stimulated cats and 0.1 mm for controls. Rapid test identified 4 of 6 stimulated cats after bPPD injection. The MAPIA detected antibody against MPB83, 16/83, 16 kDa, and M. bovis culture filtrate (MBCF) antigens. All assays differentiated between stimulated and control cats; however, 7 of 49 non-SPF control cats had a reaction for either antigen MBCF or 16/83. These preliminary studies show potential for antemortem detection of M. bovis among domestic cats. Additional studies to better characterize virulent M. bovis infection in cats would be of value.

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Improved serodetection of Mycobacterium bovis infection in badgers (Meles meles) using multiantigen test formats

Cited by 90 publications

References 25 publications

Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Accuracy of Three Diagnostic Tests for Determining Mycobacterium Bovis Infection Status in Live-Sampled Wild Meerkats (Suricata Suricatta)

Comparison of Three Immunodiagnostic Assays for Antemortem Detection of Mycobacterium Bovis Stimulation in Domestic Cats

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