Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife

Abstract

Lyashchenko, K.P.; Greenwald, R.; Esfandiari, J.; Chambers, M.A.; Vicente, J.; Gortazar, C.; Santos, N.; Correia-Neves, M.; Buddle, B.M.; Jackson, R.; O'Brien, D.J.; Schmitt, S.; Palmer, M. V.; Delahay, R.J.; and Waters, W.R., "Animal-side serologic assay for rapid detection of Mycobacterium Bovis infection in multiple species of free-ranging wildlife" (2008 AbstractNumerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n = 1532), white-tailed deer (n = 463), brushtail possums (n = 129), and wild boar (n = 177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT This article is a U.S. government work, and is not subject to copyright in the United States.may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock. #

Serologic Tests for Detecting Antibodies against Mycobacterium Bovis and Mycobacterium Avium Subspecies Paratuberculosis in Eurasian Wild Boar (Sus Scrofa Scrofa)

“…3 Also, a lateral-flow rapid test a has demonstrated a sensitivity of 77% and specificity of 97% for detecting M. bovis infection in Eurasian wild boar. 18 More recently, a new point-of-care test format for bTB that uses the innovative dual-path platform (DPP) technology a has shown enhanced specificity in elephants and red deer. 6,14 These rapid tests have practical advantages over other diagnostic tools, because they are easy to perform, can be undertaken on site, and produce results typically within 20 min.…”

TUBERCULOSIS AND BRUCELLOSIS IN WOOD BISON (BISON BISON ATHABASCAE) IN NORTHERN CANADA: A RENEWED NEED TO DEVELOP OPTIONS FOR FUTURE MANAGEMENT

“…c-Interferon assays also have broad utility in cattle testing, but little work has been performed in bison (Schiller et al 2010), although these tests were useful in African buffalo (Syncerus caffer; Michel et al 2011). Combinations of cell-mediated tests and serologic tests offer optimal sensitivity for diagnosis of bTB in most wildlife species (Lyashchenko et al 2008;Buddle et al 2009Buddle et al , 2010Chambers et al 2009;Drewe et al 2009), and that may apply to bison as well. Importantly, tests for differentiating infected from vaccinated animals were developed in cattle and could be adapted for use in free-ranging bison (Vordermeier et al 2009;Jones et al 2012;Waters et al 2012).…”

Comparison of Three Immunodiagnostic Assays for Antemortem Detection of Mycobacterium Bovis Stimulation in Domestic Cats

“…Recent studies have proven the rapid test to be useful in detecting M. bovis infections in llamas (Llama glama), elk (Cervus elaphus), white-tailed deer (Odocoileus virginianus), wild boar (Sus scrofa), Eurasian badgers, brushtail possums (Trichosurus vulpecula), and meerkats (Suricata suricatta); however, validation of the rapid test for domestic cats has yet to be achieved. 6,9,10,12,16,17,26 Multiple antigen print immunoassay identified 16/83, 16 kDa, MPB83, and MBCF as major antigens involved in the humoral immune response in sensitinogen-stimulated cats. Visually all sensitinogen-stimulated cats reacted consistently to antigen MBCF and less consistently to 16/ 83, 16 kDa, and MPB83 antigens (Fig.…”