Improved sensitivity for detection of tuberculosis cases by a modified Anda-TB ELISA test

Al-Hajjaj, Mohamed S.; Gadelrab, Mohammad Osman; Al-Orainey, Ibrahim O.; Al-Kassimi, Feisal A.

doi:10.1054/tuld.1998.0205

Cited by 9 publications

(10 citation statements)

References 30 publications

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“…In all these three groups significantly low positivity using ERBA LISA test was obtained i. (2)(3)(4)(5). Our findings are in correlation with these authors.…”

Section: Resultssupporting

confidence: 82%

Serodiagnosis of tuberculosis using two ELISA systems

Banerjee

Gupta

Shende

et al. 2003

Indian J Clin Biochem

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Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteda, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified from M. tb H3~Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-udnary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.

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“…In all these three groups significantly low positivity using ERBA LISA test was obtained i. (2)(3)(4)(5). Our findings are in correlation with these authors.…”

Section: Resultssupporting

confidence: 82%

Serodiagnosis of tuberculosis using two ELISA systems

Banerjee

Gupta

Shende

et al. 2003

Indian J Clin Biochem

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“…From the literature searches, we identified 3,720 citations, of which 27 publications (68 studies) met our eligibility criteria (Figure 1) [38–64]. We considered most studies to be distinct ([43], study b, is a substudy).…”

Section: Resultsmentioning

confidence: 99%

“…Figure 6 shows the forest plots for studies using Anda-TB IgG for the diagnosis of pulmonary TB in smear microscopy–positive patients (ten studies) [38,40,41,51,52,56,59,61,62,64]. Figure S5 shows the corresponding SROC curve.…”

Section: Resultsmentioning

confidence: 99%

“…Figure S5 shows the corresponding SROC curve. Sensitivity values ranged from 63% to 85%, with sensitivity ≤ 80% in four (40%) studies [38,51,62,64]; specificity values ranged from 73% to 100%, with specificity < 90% in four (40%) studies [52,61,62,64]. The AUC was 0.86 (95% CI 0.77–0.95), which was lower than the value noted in studies of smear microscopy–positive patients for all commercial tests combined.…”

Section: Resultsmentioning

confidence: 99%

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Commercial Serological Antibody Detection Tests for the Diagnosis of Pulmonary Tuberculosis: A Systematic Review

et al. 2007

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BackgroundThe global tuberculosis epidemic results in nearly 2 million deaths and 9 million new cases of the disease a year. The vast majority of tuberculosis patients live in developing countries, where the diagnosis of tuberculosis relies on the identification of acid-fast bacilli on unprocessed sputum smears using conventional light microscopy. Microscopy has high specificity in tuberculosis-endemic countries, but modest sensitivity which varies among laboratories (range 20% to 80%). Moreover, the sensitivity is poor for paucibacillary disease (e.g., pediatric and HIV-associated tuberculosis). Thus, the development of rapid and accurate new diagnostic tools is imperative. Immune-based tests are potentially suitable for use in low-income countries as some test formats can be performed at the point of care without laboratory equipment. Currently, dozens of distinct commercial antibody detection tests are sold in developing countries. The question is “do they work?”Methods and FindingsWe conducted a systematic review to assess the accuracy of commercial antibody detection tests for the diagnosis of pulmonary tuberculosis. Studies from all countries using culture and/or microscopy smear for confirmation of pulmonary tuberculosis were eligible. Studies with fewer than 50 participants (25 patients and 25 control participants) were excluded. In a comprehensive search, we identified 68 studies. The results demonstrate that (1) overall, commercial tests vary widely in performance; (2) sensitivity is higher in smear-positive than smear-negative samples; (3) in studies of smear-positive patients, Anda-TB IgG by enzyme-linked immunosorbent assay shows limited sensitivity (range 63% to 85%) and inconsistent specificity (range 73% to 100%); (4) specificity is higher in healthy volunteers than in patients in whom tuberculosis disease is initially suspected and subsequently ruled out; and (5) there are insufficient data to determine the accuracy of most commercial tests in smear microscopy–negative patients, as well as their performance in children or persons with HIV infection.ConclusionsNone of the commercial tests evaluated perform well enough to replace sputum smear microscopy. Thus, these tests have little or no role in the diagnosis of pulmonary tuberculosis. Lack of methodological rigor in these studies was identified as a concern. It will be important to review the basic science literature evaluating serological tests for the diagnosis of pulmonary tuberculosis to determine whether useful antigens have been described but their potential has not been fully exploited. Activities leading to the discovery of new antigens with immunodiagnostic potential need to be intensified.

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“…(1) Kecerdasan emosional adalah "satu unit perilaku, kemampuan, keyakinan, dan nilai-nilai yang kompleks, yang membuat manusia mampu memahami imajinasi dan tugas-tugasnya dengan sukses sesuai dengan pilihannya". (2) Berdasarkan pengertian diatas, dapat disimpulkan bahwa kecerdasan emosional adalah kemampuan mengenali perasaan kita sendiri dan perasaan orang lain, kemampuan memotivasi diri sendiri, kemampuan mengelola emosi dengan baik pada diri sendiri dan mengenali dengan baik emosi orang lain.…”

Section: Kajian Pustakaunclassified

Menumbuhkan Minat Berwirausaha Mahasiswa Melalui Kecerdasan Emosional

Halimah

Wanto

Mahmuddin

2018

J-SAM

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This study was conducted to determine the effect of emotional intelligence on students' entrepreneurship interest at semester VI Department of Information Systems Study Program Information System of Sampit Darwan Ali University Academic Year 2016/2017. This study used quantitative methods using survey technique. The subjects of the study were 92 students. Data collection techniques use a questionnaire fulfilled the requirements of validity and reliability. The technique of validity test used correlated item-total correlation and reliability test of Cronbach's alpha. In this study, the instrument used was questionnaire by using the likert scale. The data analysis technique used was a simple linear regression analysis fulfilled the analytical prerequisite, such as the test of normality, linearity and autocorrelation. This study revealed that emotional intelligence was moderate 70.65% and entrepreneurship interests were also moderate 67.39%. The result of analysis showed that there was an influence of emotional intelligence to student entrepreneur interest. Coefficient of determination (r2) was 0.265. It meant that emotional intelligence could foster interest in entrepreneurship student.

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Improved sensitivity for detection of tuberculosis cases by a modified Anda-TB ELISA test

Cited by 9 publications

References 30 publications

Serodiagnosis of tuberculosis using two ELISA systems

Serodiagnosis of tuberculosis using two ELISA systems

Commercial Serological Antibody Detection Tests for the Diagnosis of Pulmonary Tuberculosis: A Systematic Review

Menumbuhkan Minat Berwirausaha Mahasiswa Melalui Kecerdasan Emosional

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