Abstract:One of the most common methods to treat thromboembolism is the use of thrombolytic drugs to activate fibrinolytic protease.T he aim of this treatment was to initiate the lysis of fibrin;h owever,t here are many side-effects associated with this form of treatment. Herein, we fabricated chiral Co 3 O 4 supraparticles (SPs) with ag-factor of up to 0.02 at 550 nm and paramagnetic performance applied in the treatment of thromboembolism under an electromagnetic field (MF). In vitro experiments showed that d-SPs degr… Show more
“…Chiroptical activity, as a nascent optical property of nanomaterials, has drawn significant levels of attention over the past few years − and can produce a susceptible signal response to external changes. − As such, chiroptical activity has been promoted widely for chiral analysis and detection, along with enantioselective separation. − However, the responsiveness of monodisperse chiral inorganic nanomaterials to biological signaling molecules, such as ROS, and their direct application as biosensors for real-time monitoring, has yet to be elucidated.…”
Biological application of chiral nanoparticles (NPs) has aroused enormous levels of attention over recent years. Here, we synthesized magneto-chiral cobalt hydroxide (Co(OH) 2 ) NPs that exhibited strong chiroptical and unique magnetic properties and applied these NPs to detect and monitor reactive oxygen species (ROS) in living cells and in vivo. Circular dichroism (CD) and magnetic resonance imaging (MRI) signals of the magneto-chiral Co(OH) 2 NPs exhibited a wide intracellular ROS detection range from 0.673 to 612.971 pmol/10 6 cells with corresponding limits of detection (LOD) at 0.087 and 0.179 pmol/10 6 cells, far below that of currently available probes; the LOD for D-aspartic acid coated Co(OH) 2 NPs (D-Co(OH) 2 NPs) was 5.7 times lower than that for L-aspartic acid coated Co(OH) 2 NPs (L-Co(OH) 2 NPs) based on the CD signals. In addition, D-Co(OH) 2 NPs also exhibited dynamic ROS monitoring ability. The high levels of selectivity and sensitivity to ROS in complex biological environments can be attributed to the Co 2+ oxidation reaction on the surface of the NPs. Furthermore, magneto-chiral Co(OH) 2 NPs were able to quantify the levels of ROS in living mice by fluorescence and MRI signals. Collectively, these results reveal that magneto-chiral Co(OH) 2 NPs exhibit a remarkable ability to quantify ROS levels in living organisms, and could therefore provide new tools for exploring chiral nanomaterials as a potential biosensor to investigate biological events.
“…Chiroptical activity, as a nascent optical property of nanomaterials, has drawn significant levels of attention over the past few years − and can produce a susceptible signal response to external changes. − As such, chiroptical activity has been promoted widely for chiral analysis and detection, along with enantioselective separation. − However, the responsiveness of monodisperse chiral inorganic nanomaterials to biological signaling molecules, such as ROS, and their direct application as biosensors for real-time monitoring, has yet to be elucidated.…”
Biological application of chiral nanoparticles (NPs) has aroused enormous levels of attention over recent years. Here, we synthesized magneto-chiral cobalt hydroxide (Co(OH) 2 ) NPs that exhibited strong chiroptical and unique magnetic properties and applied these NPs to detect and monitor reactive oxygen species (ROS) in living cells and in vivo. Circular dichroism (CD) and magnetic resonance imaging (MRI) signals of the magneto-chiral Co(OH) 2 NPs exhibited a wide intracellular ROS detection range from 0.673 to 612.971 pmol/10 6 cells with corresponding limits of detection (LOD) at 0.087 and 0.179 pmol/10 6 cells, far below that of currently available probes; the LOD for D-aspartic acid coated Co(OH) 2 NPs (D-Co(OH) 2 NPs) was 5.7 times lower than that for L-aspartic acid coated Co(OH) 2 NPs (L-Co(OH) 2 NPs) based on the CD signals. In addition, D-Co(OH) 2 NPs also exhibited dynamic ROS monitoring ability. The high levels of selectivity and sensitivity to ROS in complex biological environments can be attributed to the Co 2+ oxidation reaction on the surface of the NPs. Furthermore, magneto-chiral Co(OH) 2 NPs were able to quantify the levels of ROS in living mice by fluorescence and MRI signals. Collectively, these results reveal that magneto-chiral Co(OH) 2 NPs exhibit a remarkable ability to quantify ROS levels in living organisms, and could therefore provide new tools for exploring chiral nanomaterials as a potential biosensor to investigate biological events.
“…Chirality widely exists in living systems and plays important roles in many biological and physiological processes. NPs with distinct chirality have shown attractive potential in many fields, such as catalysis, sensing, , and disease therapy. , When chiral nanomaterials enter the body, there will be stereoselective interactions between the chiral nanointerface and biomacromolecules. Thus, it is extremely important to elucidate the effect of chirality on NP–protein interactions and the underlying mechanisms.…”
Elucidating the biological behavior of engineered nanoparticles, for example, the protein corona, is important for the development of safe and efficient nanomedicine, but our current understanding is still limited due to its highly dynamic nature and lack of adequate analytical tools. In the present work, we demonstrate the establishment of a fluorescence resonance energy transfer (FRET)-based platform for monitoring the dynamic evolution behavior of the protein corona in complex biological media. With human serum albumin and lysozyme as the model serum proteins, the protein exchange process of the preformed corona on the surface of chiral quantum dots (QDs) upon feeding either individual protein or human serum was monitored in situ by FRET. Important parameters characterizing the evolution process of protein corona could be obtained upon quantitative analysis of FRET data. Further combining real-time FRET monitoring with gel electrophoresis experiments revealed that the nature of the protein initially adsorbed on the surface of QDs significantly affects the subsequent dynamic exchange behavior of the protein corona. Furthermore, our results also revealed that only a limited proportion of proteins are involved in the protein exchange, and the exchange process exhibits a significant dependence on the surface chirality of QDs. This work demonstrates the feasibility of FRET as a powerful tool to exploit the dynamic evolution process of the protein corona, which can provide theoretical guidance for further design of advanced nanomaterials for biomedical applications.
“…The research group of Xu and Kuang investigated the construction of chiral nanoprobes and their biological effects, [92][93][94] and comprehensively studied the interactions between chiral nanomaterials and biosystems. An ultrasensitive detection method for biomolecules, including biomarkers of disease, biotoxins, bacteria, metal ions, and markers of cell metabolism, was established with self-assembled plasmonic chiral probes, based on their strong optical activity in the visible spectrum (Fig.…”
Section: Chiral Biosensor and Their Biological Effectsmentioning
Chirality is a ubiquitous physical attribute in nature. Since the development of nanomaterials, the considerable interest in this research field has increased, extending the study of chirality to the nanoscale....
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