Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers

Rodrigues, Rafael C.; Bolívar, Juan M.; Volpato, Giandra; Filice, Marco; Godoy, César A.; Guisán, José M.

doi:10.1016/j.jbiotec.2009.09.002

Cited by 30 publications

(20 citation statements)

References 35 publications

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“…For the amino-dextran (AMD) preparation, 18 aldehydedextran was first obtained by oxidation of 1.67 g of dextran (average mol wt 15 000-30 000, Aldrich) dissolved in water (50 cm 3 ) with 0.8 g of sodium periodate (NaIO 4 , ≥99.0%, Aldrich). The oxidation was performed overnight at room temperature under magnetic stirring.…”

Section: Methodsmentioning

confidence: 99%

Surface modified Eu:GdVO4 nanocrystals for optical and MRI imaging

et al. 2013

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A facile solvothermal route has been developed for the preparation of europium doped gadolinium orthovanadate nanoparticles (∼70 nm) with tetragonal structure, based on a homogenous precipitation reaction at 120°C from rare earth precursors (yttrium nitrate and europium nitrate) and sodium orthovanadate solutions using an ethylene glycol-water mixture as the solvent. The effects of the doping level on the luminescence properties were evaluated in order to find the optimum nanophosphors. These nanocrystals were successfully functionalized with amino (two step process) and carboxylate (one-pot process) groups provided by amino-dextran polymers (AMD) and polyacrylic acid (PAA), respectively. It was found that while the luminescent properties of both kinds of functionalized systems were similar, the colloidal stability of the PAA-modified sample was higher, because of which, it was selected to study their cytotoxicity and magnetic properties (relaxivity and phantom analyses) to assess their potentiality as multifunctional probes for both "in vitro" optical biolabels and negative contrast agents for magnetic resonance imaging.

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Section: Methodsmentioning

confidence: 99%

Surface modified Eu:GdVO4 nanocrystals for optical and MRI imaging

et al. 2013

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“…In fact, if several enzyme-support linkages are established, the refolding may be facilitated because the relative positions of these groups cannot be altered, and those may act as reference points during refolding [29]. Even heavily chemically modified enzymes could be unfolded and refolded after immobilization via multipoint covalent attachment [29,30]. The main requirements to use this strategy are that the enzyme should remain attached to the support during all the reactivation steps, and that the support is inert enough to avoid undesired enzyme-support interactions.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of lipases on glyoxyl–octyl supports: Improved stability and reactivation strategies

Suescun

Rueda

Santos

et al. 2015

Process Biochemistry

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“…Furthermore, the support surface will never be "inert", and this can produce undesired enzyme supports/interactions during storage or use. This kind of immobilization may not be compatible with folding/unfolding reactivation strategies of enzymes: unfolding may produce enzyme release, whereas refolding will be affected by interactions with the support [4,[49][50][51][52] (Fig. 6).…”

Section: Immobilization Via Physical Adsorptionmentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers

Cited by 30 publications

References 35 publications

Surface modified Eu:GdVO4 nanocrystals for optical and MRI imaging

Surface modified Eu:GdVO4 nanocrystals for optical and MRI imaging

Immobilization of lipases on glyoxyl–octyl supports: Improved stability and reactivation strategies

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

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