Improved quantification of monosaccharides in complex lignocellulosic biomass matrices: A gas chromatography-mass spectrometry based approach

Zweckmair, Thomas; Schiehser, Sonja; Rosenau, Thomas; Potthast, Antje

doi:10.1016/j.carres.2017.04.011

Cited by 15 publications

(6 citation statements)

References 17 publications

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“…The fact that derivatization is highly matrix dependent, whereby the matrix can catalyze or suppress derivatization, strongly affects derivatization efficiency as well as the ratio of the 4TMS and 5TMS derivatives. In contrast to Gullberg et al, derivatization and injection parameters were optimized in order to steer the derivatization toward the formation of the 4TMS derivative.…”

Section: Resultsmentioning

confidence: 99%

Selective and Accurate Quantification of N-Acetylglucosamine in Biotechnological Cell Samples via GC–MS/MS and GC–TOFMS

2020

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N-Acetylglucosamine is a key component of bacterial and fungal cell walls and of the extracellular matrix of animal cells. It plays a variety of roles at the cell surface structure and is under discussion to be involved in signaling pathways. The presence of a number of N-acetylhexosamine stereoisomers in samples of biological or biotechnological origin demands for dedicated high efficiency separation methods, due to identical exact mass and similar fragmentation patterns of the stereoisomers. Gas chromatography offers high sample capacity, separation efficiency, and precision under repeatability conditions of measurement, which is a necessity for the analysis of low abundant stereoisomers in biological samples. Automated online derivatization facilitates to overcome the main obstacle for the use of gas chromatography in metabolomics, namely, the derivatization of polar metabolites prior to analysis. Using alkoximation and subsequent trimethylsilylation, carbohydrates and their derivatives are known to show several derivatives, since derivatization is incomplete as well as highly matrix dependent inherent to the high number of functional groups present in carbohydrates. A method based on efficient separation of ethoximated and trimethylsilylated N-acetylglucosamines was developed. Accurate absolute quantification is enabled using biologically derived 13C labeled internal standards eliminating systematic errors related to sample pretreatment and analysis. Due to the lack of certified reference materials, a methodological comparison between tandem and time-of-flight mass spectrometric instrumentation was performed for mass spectrometric assessment of trueness. Both methods showed limits of detection in the lower femtomol range. The methods were applied to biological samples of Penicillium chrysogenum cultivations with different matrices revealing excellent agreement of both mass spectrometric techniques.

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Section: Resultsmentioning

confidence: 99%

Selective and Accurate Quantification of N-Acetylglucosamine in Biotechnological Cell Samples via GC–MS/MS and GC–TOFMS

2020

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“…For better GC-EIMS results, the use of internal standards is advisable, for example, synthetic methylated SCFAs, synthetic or nonprotein amino acids, and nonbiological polyols or glycosides, as well as carbon-and hydrogen-labeled compounds [32,35,36].…”

Section: Derivatization Of Small Molecules For Gc-eims Analysismentioning

confidence: 99%

Chemometrics-Based TLC and GC-MS for Small Molecule Analysis: A Practical Guide

Vázquez-Martínez¹,

López²

2019

Chemometrics and Data Analysis in Chromatography

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Nowadays, thin-layer chromatography (TLC) and gas chromatography/mass spectrometry (GC-MS) instruments can produce more data than even before. At this point, the use of mathematical and statistical tools has provided the key to resolve the information overload. In this chapter, a practical guide is provided for the TLC and GC-MS analysis of short-chain fatty acids (SCFAs), amino acids, and monosaccharides. A methodology for extracting and transforming the chromatographic data to a suitable format for chemometrics is described. Furthermore, a procedure for chemometric analysis based on principal components analysis and clustering analysis is suggested.

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“…Ginkgo biloba, a key plant in China [1], is widely used to treat cardiovascular and cerebrovascular diseases in clinical practice [2][3][4]. Preparations of this plant are considered a double-action drug for the treatment of cardiovascular and neurological diseases and could alleviate cognitive impairment in the elderly population [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

A capillary electrophoresis method for the determination of soluble monosaccharides in Ginkgo biloba leaves

Liu

Yang

et al. 2021

J of Separation Science

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A method for the simultaneous determination of six monosaccharides by pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone and capillary electrophoresis was developed in this work. The derivatization (i.e., reaction temperature, capillary electrophoresis duration, and extraction number) and separation (i.e., pH and buffer concentration) conditions for capillary electrophoresis were optimized. Results showed that the limits of detection under optimal conditions were in the range of 0.036-0.35 mg/L with a mean correlation coefficient >0.99. The recoveries were in the range of 87.3-108.49%, and the relative standard deviations of intra-and inter-day variations were in the ranges of 2.2-3.8 and 3.2-5.0%, respectively. The method was successfully applied to the analysis of six free monosaccharides in three types of Ginkgo biloba leaves.

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Improved quantification of monosaccharides in complex lignocellulosic biomass matrices: A gas chromatography-mass spectrometry based approach

Cited by 15 publications

References 17 publications

Selective and Accurate Quantification of N-Acetylglucosamine in Biotechnological Cell Samples via GC–MS/MS and GC–TOFMS

Selective and Accurate Quantification of N-Acetylglucosamine in Biotechnological Cell Samples via GC–MS/MS and GC–TOFMS

Chemometrics-Based TLC and GC-MS for Small Molecule Analysis: A Practical Guide

A capillary electrophoresis method for the determination of soluble monosaccharides in Ginkgo biloba leaves

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