Improved Proton-Detected Heteronuclear Correlation Using Gradient-Enhanced z and zz Filters

Rapid and simple approach for the NMR resonance assignment of the carbohydrate chains of an intact glycoprotein Application of gradient‐enhanced natural abundance 1H‐13C HSQC and HSQC‐TOCSY to the α‐subunit of human chorionic gonadotropin

“…2A, was recorded over 16 h using a 5 mM solution of a-hCG. The high quality of this spectrum was achieved by the combination of spoiler gradients and phase cycling to suppress pulse artifacts [16,26,32]. In Fig.…”

One‐ and two‐dimensional gradient‐selected HSQMBC NMR experiments for the efficient analysis of long‐range heteronuclear coupling constants

“…Introduction of phase error is avoided in the 1D experiment through application of a selective x pulse; therefore, a phase correction factor is not required. 13 The 1D and 2D HSQMBC experiments provide excellent suppression of resonances arising from protons bound to 12 C.…”

“…The data are processed according to the echo-antiecho protocol to provide fully phase-sensitive data. 12 In the 1D version of this experiment, the evolution period is omitted and a symmetrical set of gradients with opposite amplitude (G 1 , G 2 ) surround a selective 13 C x pulse which substitutes the hard x pulse in the 2D experiment. The second gradient is followed by a similar 13 C /2 x -G 3 -1 H /2 x -υ-1 H x -G 4 pulse train as that used in the 2D experiment.…”

Comparison of Predicted Epimerases and Reductases of the Campylobacter jejuni d-altro- and l-gluco-Heptose Synthesis Pathways

“…1 H and 13 C assignments were determined from two-dimensional 1 H total correlation spectroscopy experiments (30) using a 6-kHz spinlock for 256 complex increments and natural abundance 13 C, 1 H heteronuclear single quantum correlation experiment (31,32). All spectra were processed using VnmrJ 2.1B and 1 H and 13 C chemical shifts referenced to 2,2-dimethyl-2-silapentane-5-sulfonate sodium salt standard at 0.00 ppm.…”