Improved properties of composite collagen hydrogels: protected oligourethanes and silica particles as modulators

Claudio‐Rizo, Jesús A.; Rangel-Argote, Magdalena; Muñoz-González, Pedro U.; Castellano, Laura E.; Delgado, Jorge; González‐García, Gerardo; Mata‐Mata, José L.; Mendoza-Novelo, Birzabith

doi:10.1039/c6tb01673a

Cited by 25 publications

(36 citation statements)

References 55 publications

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“…The crosslinking degree of the materials manufactured with 10% of the cross‐linker ranges from 20 to 35% as shown in Figure 2 a, according to a ninhydrin test. Previously, it was reported that isocyanate groups with a low degree of substitution in neighboring groups, such as in HDI and LDI, increase the urethane oligomerization with PEG diols and the collagen crosslinking, compared to the isocyanate groups sterically hindered . The present results suggest that sodium‐carbamoyl sulfonate‐ended‐species in trifunctional oligourethanes derived from glycerol ethoxylate and HDI or LDI (Figure ) were also feasible for crosslinking collagen materials.…”

Section: Resultssupporting

confidence: 49%

“…The trifunctional oligourethane synthesis was carried out as previously described and is schematically depicted in Figure 1 . Briefly, the poly(ethylene oxide) (PEO) based on glycerol ethoxylate (0.01 mol, M n 1000 g mol −1 ) was added into a preheated 50 °C flask and kept for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…Silica–collagen hybrid materials manufactured in different ways have recently received considerable attention as templates to induce a particular cell fate and to treat chronic wounds . The silica nanoparticulate debris and cryptic peptides released from biomaterials containing silicon and collagen, respectively, can reach and penetrate the cell surface affecting its behavior. This process may depend on material properties such as porosity, surface chemistry, swelling, biodegradation, and mechanics.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, the structure, degradation, and mechanical properties of the composite collagen hydrogels depended on the extension of the crosslinking. In addition, the methodology previously described is compatible with the incorporation of silica particles in hydrogels by the polycondensation of colloidal silica . Former results indicated that a higher crosslinking index in collagen materials crosslinked with bifunctional oligourethanes derived from l ‐lysine diisocyanate (LDI) and hexamethylene diisocyanate (HDI) improved the mechanical properties and reduced the pore size compared to those derived from diisocyanates with reactive groups impeded by steric hindrance .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the methodology previously described is compatible with the incorporation of silica particles in hydrogels by the polycondensation of colloidal silica . Former results indicated that a higher crosslinking index in collagen materials crosslinked with bifunctional oligourethanes derived from l ‐lysine diisocyanate (LDI) and hexamethylene diisocyanate (HDI) improved the mechanical properties and reduced the pore size compared to those derived from diisocyanates with reactive groups impeded by steric hindrance . Furthermore, when hydrogels were crosslinked with LDI‐derived oligourethane, swelling and degradation rate were higher than in those HDI derivatives but less than in plain collagen.…”

Section: Introductionmentioning

confidence: 99%

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Design of Silica‐Oligourethane‐Collagen Membranes for Inflammatory Response Modulation: Characterization and Polarization of a Macrophage Cell Line

Muñoz-González

Rivera-Debernardi

Mendoza-Novelo

et al. 2018

Macromolecular Bioscience

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The polarization of macrophages M0 to M1 or M2 using molecules embedded in matrices and hydrogels is an active field of study. The design of biomaterials capable of promoting polarization has become a paramount need nowadays, since in the healing process macrophages M1 and M2 modulate the inflammatory response. In this work, several immunocytochemistry and ELISA tests strongly suggest the achievement of polarization using collagen-based membranes crosslinked with tri-functionalized oligourethanes and coated with silica. Measuring the amount of TGF-β1 secreted to culture media by macrophages growth on these materials, and quantifying the macrophage morphology, it is proved that it is possible to stimulate the anti-inflammatory pathway toward M2, having measurements with p ≤ 0.05 of statistical significance between the control and the collagen-based membranes. Furthermore, some physicochemical characteristics of the hybrid materials are tested envisaging future applications: collagenase degradation resistance, water uptake, collagen fiber diameter, and deformation resistance are increased for all the crosslinked biomaterials. It is considered that the biological and physicochemical properties make the material suitable for the modulation of the inflammatory response in the chronic wounds and promising for in vivo studies.

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Section: Resultssupporting

confidence: 49%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Design of Silica‐Oligourethane‐Collagen Membranes for Inflammatory Response Modulation: Characterization and Polarization of a Macrophage Cell Line

Muñoz-González

Rivera-Debernardi

Mendoza-Novelo

et al. 2018

Macromolecular Bioscience

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Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Claudio‐Rizo

Hernandez‐Hernandez

Cano-Salazar

et al. 2020

J of Applied Polymer Sci

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The development of collagen hydrogels with tailored properties for improved applications in biomedicine represents an area of opportunity for materials science. The collagen can form semi-interpenetrated networks (semi-IPN) with various natural and/or synthetic polymers. This work aims the preparation of novel hydrogels generated from a collagen matrix cross-linked with polyurethane (PU), and the subsequent inclusion of polysaccharide chains to form semi-IPN systems with improved properties. The choice of polysaccharides for this purpose is related to their ability to modulate the biocompatibility and the antibacterial capacity in various biomedical strategies. The work

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Decellularization of porcine esophageal tissue at three diameters and the bioscaffold modification with EETs‐ECM gel

Orozco-Vega

Montes-Rodríguez

Luévano-Colmenero

et al. 2022

J Biomedical Materials Res

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Damaged complex modular organs repair is a current clinical challenge in which one of the primary goals is to keep their biological response. An interesting case of study it is the porcine esophagus since it is a tubular muscular tissue selected as raw material for tissue engineering. The design of esophageal constructs can draw on properties of the processed homologous extracellular matrix (ECM). In this work, we report the decellularization of multilayered esophagus tissue from 1‐, 21‐ and 45‐days old piglets through the combination of reversible alkaline swelling and detergent perfusion. The bioscaffolds were characterized in terms of their residual composition and tensile mechanical properties. The biological response to esophageal submucosal derived bioscaffolds modified with ECM gel containing epoxyeicosatrienoic acids (EETs) was then evaluated. Results suggest that the composition (laminin, fibronectin, and sulphated glycosaminoglycans/sGAG) depends on the donor age: a better efficiency of the decellularization process combined with a higher retention of sGAG and fibronectin is observed in piglet esophageal scaffolds. The heterogeneity of this esophageal ECM is maintained, which implied the preservation of anisotropic tensile properties. Coating of bioscaffolds with ECM gel is suitable for carrying esophageal epithelial cells and EETs. Bioactivity of EETs‐ECM gel modified esophageal submucosal bioscaffolds is observed to promote neovascularization and antiinflammatory after rabbit full‐thickness esophageal defect replacement.

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Improved properties of composite collagen hydrogels: protected oligourethanes and silica particles as modulators

Abstract: This paper reports the structure–property relationship of novel biomedical hydrogels derived from collagen, water-soluble oligourethanes, and silica.

Cited by 25 publications

References 55 publications

Design of Silica‐Oligourethane‐Collagen Membranes for Inflammatory Response Modulation: Characterization and Polarization of a Macrophage Cell Line

Design of Silica‐Oligourethane‐Collagen Membranes for Inflammatory Response Modulation: Characterization and Polarization of a Macrophage Cell Line

Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Decellularization of porcine esophageal tissue at three diameters and the bioscaffold modification with EETs‐ECM gel

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