Improved properties of bone and cartilage tissue from 3D inkjet-bioprinted human mesenchymal stem cells by simultaneous deposition and photocrosslinking in PEG-GelMA

Gao, Guojun; Schilling, Arndt F.; Hubbell, Karen; Yonezawa, Tomo; Truong, Danh D.; Hong, Yi; Dai, Guohao; Cui, Xiaofeng

doi:10.1007/s10529-015-1921-2

Cited by 298 publications

(229 citation statements)

References 18 publications

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“…The layer-by-layer bioprinting followed by simultaneous crosslinking ensured homogeneous cell distributions within the hydrogel matrix. High cell viability and good integration of the bioprinted constructs with the defect smoothly interfaced the osteochondral plug model 64, 115 .…”

Section: Constructing the Cartilagementioning

confidence: 98%

3D Bioprinting for Tissue and Organ Fabrication

et al. 2016

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The field of regenerative medicine has progressed tremendously over the past few decades in its ability to fabricate functional tissue substitutes. Conventional approaches based on scaffolding and microengineering are limited in their capacity of producing tissue constructs with precise biomimetic properties. Three-dimensional (3D) bioprinting technology, on the other hand, promises to bridge the divergence between artificially engineered tissue constructs and native tissues. In a sense, 3D bioprinting offers unprecedented versatility to co-deliver cells and biomaterials with precise control over their compositions, spatial distributions, and architectural accuracy, therefore achieving detailed or even personalized recapitulation of the fine shape, structure, and architecture of target tissues and organs. Here we briefly describe recent progresses of 3D bioprinting technology and associated bioinks suitable for the printing process. We then focus on the applications of this technology in fabrication of biomimetic constructs of several representative tissues and organs, including blood vessel, heart, liver, and cartilage. We finally conclude with future challenges in 3D bioprinting as well as potential solutions for further development.

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Section: Constructing the Cartilagementioning

confidence: 98%

3D Bioprinting for Tissue and Organ Fabrication

et al. 2016

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“…Originally, the majority of bioinks were natural hydrogel polymers, particularly alginate and fibrin, which when printed have compressive moduli of approximately 5 kPa 12 . Human bone and cartilage typically have moduli of about 10–20 GPa and 700 kPa, respectively 25 . In order to print tissues having similar load-bearing capacities to native bone and cartilage, PEG-based hydrogels have been printed with compressive moduli between 300–350 kPa 26 .…”

Section: 3d Printing For Craniofacial Bone Regenerationmentioning

confidence: 99%

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

et al. 2016

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The treatment of craniofacial defects can present many challenges due to the variety of tissue-specific requirements and the complexity of anatomical structures in that region. 3D-printing technologies provide clinicians, engineers and scientists with the ability to create patient-specific solutions for craniofacial defects. Currently, there are 3 key strategies that utilize these technologies to restore both appearance and function to patients: rehabilitation, reconstruction and regeneration. In rehabilitation, 3D-printing can be used to create prostheses to replace or cover damaged tissues. Reconstruction, through plastic surgery, can also leverage 3D-printing technologies to create custom cutting guides, fixation devices, practice models and implanted medical devices to improve patient outcomes. Regeneration of tissue attempts to replace defects with biological materials. 3D-printing can be used to create either scaffolds or living, cellular constructs to signal tissue-forming cells to regenerate defect regions. By integrating these three approaches, 3D-printing technologies afford the opportunity to develop personalized treatment plans and design-driven manufacturing solutions to improve aesthetic and functional outcomes for patients with craniofacial defects.

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“…Frequently used bio‐inks such as gelatin,5 alginate,6 gelatin/alginate,4 gelatin/alginate/chitosan,3 and poly(ethylene glycol) (PEG) dimethacrylate/gelatin7 that have been used in the fabrication of cell‐laden scaffolds have shown high biocompatibility for cell viability in bone repair. However, they lack osteogenic capability to promote cell differentiation and new bone formation in the absence of growth factors, which represents a clear limitation for their successful application.…”

Section: Introductionmentioning

confidence: 99%

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

et al. 2017

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An osteoblast‐laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34Li0.66Si8O20(OH)4]Na0.66, clay)/hyaluronic acid sodium salt (HA) bio‐inks, is developed by a two‐channel 3D bioprinting method. The novel biodegradable bio‐ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D‐bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio‐ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell‐laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D‐bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long‐term function.

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Improved properties of bone and cartilage tissue from 3D inkjet-bioprinted human mesenchymal stem cells by simultaneous deposition and photocrosslinking in PEG-GelMA

Abstract: Inkjet bioprinted-hMSCs in simultaneously photocrosslinked PEG-GelMA hydrogel scaffolds demonstrated an improvement of mechanical properties and osteogenic and chondrogenic differentiation, suggesting its promising potential for usage in bone and cartilage tissue engineering.

Cited by 298 publications

References 18 publications

3D Bioprinting for Tissue and Organ Fabrication

3D Bioprinting for Tissue and Organ Fabrication

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

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