Improved production, purification, and characterization of biosurfactants produced by Serratia marcescens SM3 and its isogenic SMRG‐5 strain

Abstract

In this study, the biosurfactants (Bs) production of two Serratia marcescens strains (SM3 and its isogenic SMRG-5 strain) was improved and the tenso-active agents were purified and characterized. A 2 factorial design was used to evaluate the effect of nitrogen and carbon sources on the surface tension (ST) reduction and emulsion index (EI ) of the produced Bs. Optimum Bs production by SM3 was achieved at high concentrations of carbon and nitrogen, reducing ST to 26.5 ± 0.28 dynes/cm, with an EI of 79.9 ± 0.2%. Meanwhile, the best results for SMRG-5 were obtained at low concentrations, reducing the ST to 25.2 ± 0.2 dynes/cm, with an EI of 89.7 ± 0.28%. The optimal conditions for Bs production were scaled up in a 2-L reactor, yielding 4.8 and 5.2 g/L for SM3 and SMRG-5, respectively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the presence of two different lipopeptides (hidrofobic fractions: octadecanoic and hexadecanoic acid for SM3 and SMRG5, respectively). Both strains were capable of benzo [a] pyrene removal (59% after 72 H of culture).

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications

“…Pruthi and Cameotra [39] obtained a reduction of 68–27 mN/m in mineral medium containing 2% glucose by S. marcescens . Recently, Rosas-Galván et al [24] informed a reduction to 26.5 mN/m of biosurfactant produced by S. marcescens SM3.…”

“…Serratia marcescens , a facultative bacterium belonging to the Enterobacteriaceae family, produces biosurfactant such as serrawetins that present enormous potential of application in pharmaceutical industry [19, 20]. Over the last years, several studies have reported the production of biosurfactants by S. marcescens strains using a variety of hydrophilic and hydrophobic substrates [21–24]. However, researches focusing on the use of agro-industrial waste are still few and they have been carried out by our research group [8, 25].…”

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

“…Both liquids were mixed by vortex for 2 min, and then left to stand for 24 h at room temperature. EI 24 percentage was determined by the height of the emulsified region (cm) divided by the total height of the liquid column and multiplied by 100 (Cooper and Goldenberg 1987;Rosas-Galván et al 2018).…”

“…Glycolipid Bs production is significantly influenced by the type and concentration of nutrients such as carbon, nitrogen, phosphorous, and the presence of certain trace elements (Ramana and Karanth 1989;Bertrand et al 2018;Da Rosa et al 2010). Additionally, physicochemical conditions such as pH, temperature, shaking, and C/N ratio are factors that influence Bs production and biomass, and thus, these parameters are important factors to evaluate for the optimization of Bs production (Roldán-Carrillo et al 2011;Cooper and Goldenberg 1987;Da Rosa et al 2010;Cortes-Sánchez et al, 2015;Leite et al 2016;Rosas-Galván et al 2018).…”

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

“…Recently, Rosas-Galván et al [51] studied the production Bs by Serratia marcescens SM3 and its isogenic SMRG5 strain. The effect of the carbon and nitrogen sources on the production of two different lipopeptides (octadecanoic and hexadecanoic acid for SM3 and SMRG5, respectively) were evaluated.…”