Improved procedure for formation of epinephrine and norepinephrine fluorophors by the trihydroxyindole reaction

In rat adrenal gland incubated in vitro, phenylephrine and naphazoline inhibited the release of catecholamines (CA) induced in a medium containing K+ (5.6mM). In K+-free medium or in the presence of ouabain (10(-3) M) phenylephrine and naphazoline had no effect on CA release. The alpha-adrenergic blockers, phentolamine and phenoxybenzamine, increased CA release in a K+-medium but had no effect on CA release from adrenal medulla in a K+-free or ouabain (10(-3) M) containing medium. PGE2 inhibited the release of CA both in the presence and in the absence of K+, as well as in the presence of ouabain (10(-3) M). Activation of alpha-adrenergic receptors in the adrenal medulla by phenylephrine and naphazoline resulted in increased Na, K-ATPase activity but no effect on Mg-ATPase. PGE2 acts through a different mechanism.

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“…CA were assayed by the trihydroxyindole method as modified by Valori et at. [11] and by Weit-Malherbe and Bigelow [12]. Fig.…”

Section: Methodsmentioning

confidence: 91%

Inhibition of catecholamine release by alpha-adrenergic activation: Interaction with Na, K-ATPase

Gutman

Boonyaviroj²

1977

J. Neural Transmission

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“…DA was analyzed fluorometrically in one portion according to a modification of the methods of Carlsson and Waldeck" and Anton and Sayre. 15 The remaining portion was placed on Amberlite columns from which NE was eluted by 2/3 M boric acid and analyzed fluorometrically using the trihydroxyindole reaction according to modifications of the method of Renzini et al 16 and Valori et al 17 HVA and 5HIAA were organically extracted from CSF (usually 2.2 ml) using a modified combination of the methods of Ashcroft et al 18 and Gerbode and Bowers. 19 Both compounds were fluorometrically assayed, 5HIAA by the technique of Ashcroft and Sharman 20 and HVA according to Curzon et al 21 Cyclic AMP in CSF was analyzed by a protein binding assay similar to radioimmunoassay by a combination of the methods of Gilman 22 and of Brown et al 23 Glucose was measured in arterial and cerebral venous blood by an enzymatic spectrophotometric technique.…”

Section: Biochemical Methodologymentioning

confidence: 99%

Influence of adrenergic receptor blockade on circulatory and metabolic effects of disordered neurotransmitter function in stroke patients.

Meyer¹,

Miyakawa²,

Welch³

et al. 1976

Stroke

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SUMMARY Cerebral hemispheric blood flow and metabolism were measured before and after therapy with intracarotid infusion of combined PBZ and PPL in IS patients with recent cerebral infarction. HBF was unaltered despite decrease in cerebral perfusion pressure. Cerebral hemispheric oxygen consumption and carbon dioxide production decreased while cerebral hemispheric lactate production increased.Biphasic cerebral uptake of tyrosine was observed during and immediately after PBZ and PPL infusion. CSF HVA increased, indicating altered DA turnover. CSF SHIAA levels also increased, suggesting altered SHT turnover after PBZ and PPL. Release of cyclic AMP from ischemic brain into cerebral venous blood seen in the steady state was abolished after therapy.Cerebral hemodynamic studies suggest a functional balance between monoaminergic neurogenic influences in the control of cerebral circulation. Imbalance of such controlling factors in ischemic brain may lead to paradoxical vascular responses to induced hypertension and hypotension. PBZ and PPL enhance such responses perhaps by increasing central neurotransmitter turnover and release.Further shift toward cerebral anaerobic metabolism may occur in ischemic brain following the use of phenoxybenzamine and propranolol.Worsening of neurological deficit occurred in four cases. Combined therapy with PBZ and PPL does not appear beneficial in the therapy of patients with recent stroke.

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“…Urinary sodium content was determined by flame photometry. Urinary excretion of free adrenaline and noradrenaline was assessed by photofluoremetric method [17] and Methods expressed as micrograms per gram creatinine per 24 hours ( g/g Cr/24 h). The objective of the present prospective longitudinal study was After initial evaluation, all women underwent periodic clinical to investigate into the role of aging in blood pressure trends in a visits, blood pressure measurement, anthropometric examination and group of 144 nuns living in a secluded order in six nunneries in humoral investigation.…”

mentioning

confidence: 99%

Blood Pressure in Nuns in a Secluded Order: A 30-Year Follow-Up

Timio¹,

Saronio²,

Venanzi³

et al. 1999

Mineral Electrolyte Metab

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The powerful effect of psychosocial and acculturating influences on population blood pressure trends seems to be confirmed, through longitudinal observations, in the nuns in a secluded order. After initial observations had been made on culture, body form, blood pressure, diet, and other variables in 144 nuns and 138 lay women, included as a control group, a 30-year follow-up study was undertaken. Most striking were opposite trends noted between the two groups in blood pressure trend. During the follow-up period, blood pressure remained remarkably stable among the nuns. None showed an increase in diastolic blood pressure over 90 mm Hg. By contrast, the control women showed the expected increase in blood pressure with age. This resulted in a gradually greater difference (δ>30/15 mm Hg) in systolic and diastolic blood pressure between the two groups, which was statistically significant. In addition, cardiovascular morbidity and mortality, expressed as the outcome of fatal and nonfatal events, were different in the two groups. They were significantly more common in the lay women than in the nuns. Comparisons between survivalcurves were statistically significant (p = 0.0043 for fatal events; p = 0.0056 for nonfatal events) between the two groups. In conclusion, it seems reasonable to attribute much of the difference in blood pressure and cardiovascular events, to the different burden in psychosocial factor and to the preserved peaceful lifestyle of the nuns.

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Improved procedure for formation of epinephrine and norepinephrine fluorophors by the trihydroxyindole reaction

Cited by 47 publications

References 9 publications

Inhibition of catecholamine release by alpha-adrenergic activation: Interaction with Na, K-ATPase

Inhibition of catecholamine release by alpha-adrenergic activation: Interaction with Na, K-ATPase

Influence of adrenergic receptor blockade on circulatory and metabolic effects of disordered neurotransmitter function in stroke patients.

Blood Pressure in Nuns in a Secluded Order: A 30-Year Follow-Up

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