Improved pregnancy rate after transfer of embryos grown in human fallopian tubal cell coculture

Bongso, Ariff; Ng, Soon-Chye; Fong, Chui‐Yee; Anandakumar, C.; Marshall, Barry; Edirisinghe, Rohini; Ratnam, S. S.

doi:10.1016/s0015-0282(16)55265-0

Cited by 96 publications

(33 citation statements)

References 16 publications

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“…The hypothesis that the oviductal GnRH in mammals is likely to enhance embryonic development and implantation through a paracrine action (Casan et al 2000) is supported by the fact that preimplantation human/mouse embryos cultured in vitro exhibit a delay in development in comparison to their in vivo counterparts (Tazuki and Guidice 1996). However, this delay improves when cultured in a medium containing GnRH (Funston and Seidel 1995) or cocultured with fallopian tube epithelium (Bongso et al 1992;Yeung et al 1996). Increased expression of GnRH and its mRNA in the blastocyst stage, in comparison to the morula stage in cultured human and mouse embryos, as well as the expression of GnRH receptors in all embryonic developmental stages Raga et al 1999), strengthens the argument for a role of GnRH in embryonic development in mammals.…”

Section: Discussionmentioning

confidence: 99%

Localization of Immunoreactive Gonadotropin-releasing Hormone and Relative Expression of Its mRNA in the Oviduct During Pregnancy in Rats

Sengupta

Baker

Chakrabarti

et al. 2007

J Histochem Cytochem.

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S U M M A R Y This study was designed to determine the cellular and ultrastructural distribution of the gonadotropin-releasing hormone (GnRH) and the relative expression of its mRNA in the oviduct of rats during different time points (days 7, 9, 16, and 20) of pregnancy. Immunofluorescent localization and confocal microscopic techniques were used to determine the cellular distribution of GnRH in the oviduct. Immunogold electron microscopy indicated its localization at the ultrastructural level, and real-time PCR was used to study the expression pattern of GnRH mRNA in the oviduct during pregnancy. In general, GnRH was localized within the epithelial cells lining the oviductal lumen at each selected time point. A strong correlation between the fluorescence intensity of GnRH-immunoreactive cells and the relative expression of GnRH mRNA was noted on days 7 and 16, followed by a plateau by day 20. At the ultrastructural level, uniform labeling of colloidal gold particles was observed in secretory vesicles and lamella of the luminal epithelium as well as the lumen of the oviduct.Collectively, these results demonstrate for the first time that the oviductal epithelium synthesizes and secretes the decapeptide GnRH during pregnancy in rats, which may have a possible role in postimplantation embryonic development and the maintenance of pregnancy. (J Histochem Cytochem 55:525-534, 2007)

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Section: Discussionmentioning

confidence: 99%

Localization of Immunoreactive Gonadotropin-releasing Hormone and Relative Expression of Its mRNA in the Oviduct During Pregnancy in Rats

Sengupta

Baker

Chakrabarti

et al. 2007

J Histochem Cytochem.

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“…First, clinical reports indicate greater success rates for gamete and zygote intrafallopian transfer, two techniques where fertilizatiodembryo development occur within the oviduct, compared to in vitro fertilizationembryo transfer (American Fertility Society, 1992). Second, embryos from several species, including humans, have been cocultured with oviduct cells in organ, explant, and/or cell monolayer cultures (Bongso et al, 1991(Bongso et al, , 1992Yeung et al, 1992). In general, coculture studies have reported enhanced embryo development (ie., faster cleavage rates, lower percent fragmentation) when embryos were incubated with oviductal cells compared to media alone.…”

Section: Discussionmentioning

confidence: 99%

A human oviduct‐specific glycoprotein: Synthesis, secretion, and localization during the menstrual cycle

O'Day-Bowman

Mavrogianis

Fazleabas

et al. 1995

Microscopy Res & Technique

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The major objective of this study was to examine the hormonal regulation of a human oviduct-specific glycoprotein (huOGP) throughout the menstrual cycle and in all regions of the human oviduct. Regulation of synthesis and secretion was examined at both the protein (Western immunoblots and immunocytochemistry) and mRNA (Northern and slot blots) levels and correlated with changes in the morphological features of the oviductal epithelial cells throughout the cycle. Immunoblot analysis of oviductal fluid and explant culture media from all regions of the oviduct demonstrated that huOGP is primarily found during the follicular stage of the cycle and is not present in serum, follicular fluid, or uterine endometrium. Moreover, two-dimensional (2-D) immunoblots showed that all major isoelectric variants of huOGP observed on 2-D fluorographs are immunologically related. Light microscopic immunocytochemistry localized huOGP to oviductal secretory cells in both ampulla and isthmic regions, with the most intense immunoperoxidase staining seen in midcycle samples. Using an indirect immunogold technique at the electron microscopic level, huOGP was specifically localized to secretory granules of the ampullary and isthmic nonciliated epithelial cells. The ultrastructural characteristics of these secretory cells during the mid to late follicular phase of the cycle suggested elevated protein synthetic activity. In addition, mRNA expression for huOGP was elevated in all regions of the oviduct in midcycle specimens. Collectively, these data indicate that huOGP is a major tissue-specific, stage-specific secretory product of the human oviduct during the periovulatory stage of the cycle and support the hypothesis that huOGP synthesis and secretion may be regulated by fluctuations in the levels of estrogen and progesterone.

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“…Recently, it was evidenced that co-culturing the fertilized eggs with oviduct epithelial cells can extend the survival period of the eggs in in-vitro fertilization and increase the rate of implantation (Bongso et al, 1992;Freeman et al, 1993). Research regarding the basic biological data of oviduct epithelia is important in designing clinical obstetrical and gynecological treatments.…”

Section: Introductionmentioning

confidence: 99%

The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

Yan

Akutsu

Satoh

2011

Okajimas Folia Anat. Jpn.

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Improved pregnancy rate after transfer of embryos grown in human fallopian tubal cell coculture

Cited by 96 publications

References 16 publications

Localization of Immunoreactive Gonadotropin-releasing Hormone and Relative Expression of Its mRNA in the Oviduct During Pregnancy in Rats

Localization of Immunoreactive Gonadotropin-releasing Hormone and Relative Expression of Its mRNA in the Oviduct During Pregnancy in Rats

A human oviduct‐specific glycoprotein: Synthesis, secretion, and localization during the menstrual cycle

The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

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