Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

Gorinova, N.; Nedkovska, M.; Todorovska, Elena; Simova‐Stoilova, Lyudmila; Stoyanova, Z.; Georgieva, Katya; Demirevska-Kepova, K.; Atanassov, A.; Herzig, Rolf

doi:10.1016/j.envpol.2006.03.025

Cited by 51 publications

(18 citation statements)

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“…The higher PCs production in leaves of the Giza 2 cultivar indicate that much more of free Cd can penetrate into the leaves and can negatively influence the metabolic processes, e.g., photosynthesis (Gorinova et al 2007), which consequently affects the whole plant metabolism and growth. As the next step of the investigation, it would be necessary to study Cd accumulation and translocation, together with the GSH and PC tissue concentrations, during the whole life cycle of V. faba, from the germination period until the bean-fruit production.…”

Section: Correlation Between Tissue Concentrations Of CD Gsh and Pcsmentioning

confidence: 99%

Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L.

et al. 2010

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Translocation of cadmium (Cd) in the tissues of Vicia faba, the water content in biomass, the biomass production, and the glutathione and phytochelatin tissue concentrations were studied and correlated with the plant sensitivity and/or tolerance to Cd. The total concentrations of Cd were determined by inductively coupled plasma/ mass spectrometry (ICP-MS), the concentrations of glutathione (GSH) and phytochelatins 2 and 3 (PC2 and PC3) were determined by on-line high performance liquid chromatography/electrospray-ionization tandem mass spectrometry (HPLC-ESI-MS-MS) in the roots and leaves of the sensitive and the tolerant cultivars of V. faba grown in Cd containing nutrient solutions (NS, 0-100 lmol l -1 Cd 2? ). Both the cultivars of V. faba accumulate a major portion of Cd in the roots and only a minor part of ca. 4% in the leaves. The differences between the cultivars concerning Cd accumulation in leaves were apparent from higher Cd concentrations in NS and the Cd amount in the sensitive cultivar was approximately twice as high. In the roots, the differences between the cultivars in the Cd accumulation were only statistically significant with the highest Cd concentrations in NS, with the tolerant cultivar accumulating about 16% more of Cd compared to the sensitive one. The biomass production of the sensitive cultivar decreased approximately twice as fast with increasing Cd concentration in NS. The biomass water content decreased with increasing Cd concentration in NS in both the cultivars. In general, the GSH concentration did not linearly correlate with Cd accumulation, except for the roots of the sensitive cultivar where it was independent, and was higher in the sensitive cultivar than in the tolerant one in both the leaves and roots. The GSH concentration in leaves was approximately one order of magnitude higher than that in the roots for both the cultivars. The relationships between the PC and Cd concentrations in tissues were found nonlinear. At lower Cd accumulation levels, the PC concentrations followed an increase in the Cd accumulation in both the roots and leaves, whereas at higher Cd accumulations the relations differed between roots and leaves. In the roots, the PC concentrations decreased with increasing Cd accumulation, whereas the PC concentration in the leaves followed the decrease in the Cd accumulation.

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Section: Correlation Between Tissue Concentrations Of CD Gsh and Pcsmentioning

confidence: 99%

Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L.

et al. 2010

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“…in their extensive study of the accumulation of different heavy metals by plant species, reported very different behavior for shoot and root growth depending on plant species Gorinova et al (2007). observed no changes Growth of tobacco plants exposed to various Cd concentrations: fresh weight (a), dry weight (b).…”

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confidence: 99%

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

et al. 2010

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Tobacco (Nicotiana tabacum L.) is a tolerant species that accumulates cadmium. We studied the effect of Cd (0, 10, 25, 50, 100 lM) on growth parameters, chlorophyll and proline contents, enzymatic antioxidative response and lipid peroxidation of tobacco plants grown in hydroponic culture for 11 days to clarify the strategy of plant response against oxidative stress caused by this heavy metal. Cadmium accumulated more in roots than in shoots. Plant growth was not significantly affected by the cadmium concentrations used. Young leaves were more affected, showing visible chlorosis and a significant decrease in chlorophyll content at high Cd concentrations. Dry weight of both leaves and roots increased indicating a lower capacity for roots to absorb water. An increase in malondialdehyde levels was observed, indicating that lipid peroxidation occurred as a result of ROS formation. The activity of guaiacol peroxidase in leaves increased, indicating that it was very important in the scavenging of H 2 O 2 , while superoxide dismutase activity only increased in old leaves. Ascorbate peroxidase showed constant activity levels in tobacco leaves, suggesting that the ascorbate-glutathione pathway was less important as a defense mechanism.

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“…Aside from their well-established role in heavy metal homeostasis and detoxification, plant MTs may be involved in protection against intracellular oxidative stress, which underlies most other stresses (Lee et al, 2004;Gorinova et al, 2007;Xue et al, 2009). On the other hand, in some physiological or defense processes, there is a need for elevated ROS concentrations, which serve as effector or signal molecules (Wong et al, 2004).…”

Section: Discussionmentioning

confidence: 98%

Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)

Samardžić

Nikolić

Timotijević

et al. 2010

Journal of Plant Physiology

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Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

Cited by 51 publications

References 50 publications

Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L.

Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L.

Oxidative stress induced by cadmium in Nicotiana tabacum L.: effects on growth parameters, oxidative damage and antioxidant responses in different plant parts

Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)

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