Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights

Hu, Lianghai; Chen, Xueguo; Kong, Liang; Su, Xingye; Ye, Mingliang; Zou, Hanfa

doi:10.1016/j.chroma.2005.06.066

Cited by 99 publications

(45 citation statements)

References 25 publications

(33 reference statements)

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“…Because most components extracted by supercritical CO 2 were small and uncharged molecules, the available separation modes were limited. In previous reports of 2D-HPLC, a CN column was used as the first dimension column with methanol/water as the mobile phase, and satisfactory separation of the extracts of natural products was obtained [32][33]. This configuration was also adopted in the present study.…”

Section: Resultsmentioning

confidence: 98%

On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum

Zhang

Zhang²,

Duan

et al. 2006

J of Separation Science

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A novel on-line system combining supercritical fluid extraction (SFE) and two-dimensional high performance liquid chromatography (2D-HPLC) was developed. A trap column and two three-port valves were employed to couple SFE and 2D-HPLC system, which was composed of a CN column and a monolithic silica column, connected by a 10-port dual-position valve. The analytes extracted by supercritical CO2 were completely transferred to the 2D-HPLC system. After separation in two orthogonal modes, the eluents were delivered to APCI-tandem-MS for identification of the samples. In this way, sample preparation, separation, detection, and identification were integrated into an on-line system permitting analysis of the fruiting bodies of Ganoderma lucidum, and at least 73 components in the extract were resolved with calculated peak capacity of up to 1643.

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Section: Resultsmentioning

confidence: 98%

On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum

Zhang

Zhang²,

Duan

et al. 2006

J of Separation Science

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“…3). The performance of 2-D LC was further improved by using a silica monolithic column for the second-dimension separation [72]. The developed system was applied to anal- ysis of the methanol extract of two umbelliferae herbs Ligusticum chuanxiong Hort.…”

Section: Multidimensional Separation Modes For Traditional Chinese Mementioning

confidence: 99%

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Gao

Deng

Lin

et al. 2007

J of Separation Science

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ReviewRecent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicinesThe most basic task in proteomics remains the detection and identification of proteins from a biological sample, and the most traditional way to achieve this goal consists in protein separations performed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Yet the 2-D PAGE-mass spectrometry (MS) approach has its drawbacks with regard to automation, sensitivity, and throughput. Consequently, considerable effort has been devoted to the development of non-gel-based proteome separation technologies in an effort to alleviate the shortcomings of 2-D PAGE. In addition, traditional Chinese medicines (TCMs), due to their long period of clinical testing and reliable therapeutic efficacy, are attracting increased global attention. However, hundreds or even thousands of components are usually present in TCMs, which results in great difficulties of separation. As a mainstream separation tool, multidimensional liquid separation systems have shown powerful separation ability, high peak capacity, and excellent detectability in the analysis of complex samples including biological samples and TCMs, etc. Therefore, this review emphasizes the most recent advances in multidimensional liquid chromatography and capillary electrophoresis-based separation techniques, and the corresponding applications in proteomics and TCMs. In view of the significant contributions from Chinese scientists, this review focuses mainly on the work of Chinese scientists in the above fields.

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“…Development of this powerful separation technique is mostly owed to the emergence of sophisticated interfaces, which efficiently transfer the first dimension ( 1 D) eluate to the second dimension ( 2 D). Various interfaces, such as switching valves coupled with empty storage loops [6][7][8][9][10] or solid-phase extraction (SPE) columns [11][12][13], stop-flow interface [14,15] and interfaces with parallel second dimension columns [16,17] have been successfully developed for 2D LC. More simple, efficient and sophisticated interface apparatus with independent flow programs and valve switching events are needed to develop effective online 2D LC analysis methods.…”

Section: Introductionmentioning

confidence: 99%

Application of fractionized sampling and stacking for construction of an interface for online heart-cutting two-dimensional liquid chromatography

Xia

Liu

et al. 2016

Journal of Chromatography A

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Improved performance of comprehensive two-dimensional HPLC separation of traditional Chinese medicines by using a silica monolithic column and normalization of peak heights

Cited by 99 publications

References 25 publications

On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum

On‐line hyphenation of supercritical fluid extraction and two‐dimensional high performance liquid chromatography‐atmospheric pressure chemical ionization tandem mass spectrometer for the analysis of Ganoderma lucidum

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Application of fractionized sampling and stacking for construction of an interface for online heart-cutting two-dimensional liquid chromatography

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