Improved Micropropagation of Plantlets from Nodal Explants of Anchote (Coccinia abyssinica)—A Calcium- and Protein-rich Tuber

Kahia, Jane; Njenga, Peter; Kirika, Margaret

doi:10.21273/hortsci.51.7.905

Cited by 6 publications

(7 citation statements)

References 10 publications

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“…During the current study, kinetin was found to be more effective than TDZ in inducing somatic embryogenesis. These results are contrary to many studies which indicate that TDZ (a phenylurea type) is superior to the adenine types of cytokinins in inducing somatic embryogenesis in several plant species (Aboshama, 2011;Feng and Chen, 2014;Gill and Saxena, 1992;Kahia et al, 2016;Visser et al, 1992). In cacao, TDZ has been used by many workers (Ajijah et al, 2014;Garcia et al, 2016;Li et al, 1998;Maximova et al, 2002;Quainoo and Dwomo, 2012).…”

Section: Discussioncontrasting

confidence: 89%

“…The work being reported is similar to that of Mayati (2015) who found that kinetin was better than zeatin in somatic embryogenesis of Hevea brasiliensis RRIM 2025. However, it conflicts with the work of Chen and Chang (2001), Wongtiem et al (2011), and Kahia et al (2016) who reported that kinetin was inferior to other cytokinins evaluated for the induction of somatic embryos in Oncidium 'Gower Ramsey', Manihot esculenta Crantz, and Coffea Arabica, respectively. During the current study, increasing the concentration of kinetin was found to inhibit induction of somatic embryos.…”

Section: Discussionmentioning

confidence: 65%

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Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants

Kahia¹,

Koné²,

Diby³

et al. 2017

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Theobroma cacao L. (cacao) is a major tropical crop, grown for its oil-rich seed, which is used in the manufacture of chocolate, its derivatives, and cosmetics. Cacao is cultivated mainly by smallholders and represents a significant export commodity for some developing countries such as Côte d’Ivoire. It is conventionally propagated by seeds, grafting, and cuttings. Somatic embryogenesis offers an alternative method for propagation where large-scale production of planting materials is possible. In the current study, the effect of different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and kinetin on induction of somatic embryogenesis and plantlet regeneration in two cocoa clones (coded as C1 and C14) were evaluated. Flowers were collected early in the morning, sterilized, explants excised and cultured on Driver, and Kuniyuki Walnut (DKW) media supplemented with different concentrations of 2, 4-D (9, 10, and 20 µM) and kinetin (0.5, 1, 2.5, 5, 10, and 25 µM) in separate experiments. The frequently used media in somatic embryogenesis of cacao [DKW supplemented with 0.022 µM thidiazuron (TDZ) and 9 µM 2, 4-D] was used as a control. The results of the study showed that explants cultured on media supplemented with 10 µM 2, 4-D and 5 µM kinetin produced the highest (28.0 ± 1.1) mean number of embryos/explant in C1 and this was a 9-fold increase in the number of embryos compared with the control. Explants cultured on media supplemented with 10 µM 2, 4-D and 2.5 µM kinetin produced the highest (7.0 ± 4.0) mean number of embryos/explant in C14 whereas the explants cultured on media supplemented with 20 µM 2, 4-D and 2.5 µM kinetin gave the highest (22.0 ± 1.7) mean number of embryos in clone C1 and C14. The regenerated embryos were germinated and successfully weaned in the green house with a survival rate of 70% being recorded. The paper describes an improved protocol compared with previous work in terms of embryo recovery and survival rate of the elite clones of cocoa through somatic embryogenesis. The results of the current study confirm that somatic embryogenesis of cacao clones is genotype dependent.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 65%

Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants

Kahia¹,

Koné²,

Diby³

et al. 2017

horts

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“…In the work on date palm, it was found that the shoot multiplication rate increased with increasing BAP concentration, but more hyperhydricity was observed (Mazri, 2015). Another work on anchote also indicated that hyperhydric shoots were observed on media supplemented with high concentrations of BAP and kinetin (Kahia et al, 2016). Similarly, our work suggested that shoots regenerated normally on media with lower concentration of BA (0.1 mg • L -1 ), whereas hyperhydricity phenomenon appeared on media with higher concentration of BA (0.1 or 0.5 mg • L -1 ) (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…hyperhydricity induction, whereas the concentration has little influence (Kadota M and Niimi, 2003), in which synthetic phenylurea derivatives [N-(2-chloro-4-pyridyl)-N#-phenylurea (CPPU) and TDZ] produce more hyperhydric shoots than adenine derivatives (BA and kinetin). But in anchote, hyperhydrated shoots appeared on media supplemented with BAP and kinetin but not on TDZ media (Kahia et al, 2016). Therefore, exogenous cytokinin type and concentration suitable for shoot regeneration may depend on plant species.…”

Section: Resultsmentioning

confidence: 98%

In Vitro Culture of Heuchera villosa ‘Caramel’

Zhao¹,

He²,

Li³

et al. 2017

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The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

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“…Despite the importance of anchote, generally few studies have been conducted and almost nothing has been done with regard to improving the species through biotechnological applications before our first report on in vitro propagation (Yambo & Feyissa 2013) and molecular genetic diversity study using markers generated by Inter Simple Sequence Repeat (ISSR) techniques (Bekele et al 2014), which has been followed by others (Bekele et al 2013;Guma et al 2015;Kahia et al 2016;Abate et al 2019;Mekbib et al 2018). The objective of this article is to review research on anchote, present it in a comprehensive way, and suggest future directions of its improvement using biotechnological tools.…”

Section: ____________________________________________________________mentioning

confidence: 99%

Coccinia abyssinica (Lam.) Cogn. (Anchote) Biology, Productivity, and Prospects of Genetic Improvement Using Biotechnological Tools

Feyissa

2020

Journal of Horticultural Research

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Coccinia abyssinica (Lam.) Cogn. (local name anchote) is a tuber crop that belongs to the family Cucurbitaceae and it is cultivated for food and medicinal uses. It has relatively high quality of nutrient composition compared to other tuber crops, and is considered as the leading proteinous root crop with a high calcium content. Therefore, cooked anchote tubers are highly recommended for patients with broken or fractured bones. Anchote also contains alkaloids, phenols, tannins, flavonoids, and saponins. Although anchote is principally cultivated for its tubers, farmers prefer propagation by seeds as they are easy to store. Farmers select high-quality fruits for future seeds, based on the size of fruits and tubers. Since diseases and pests rarely affect the tubers, protection is not common. However, the fruit fly can damage the fruits, which predisposes them to decay. Although anchote has very high potential as a food security crop, it is neglected and underutilized and has received very limited research attention. Research published so far covers its ethnobotany, nutritional and anti-nutritional composition, traditional methods of reproduction, in vitro reproduction, somatic embryogenesis, anther breeding, and morphological and molecular genetic diversity. This article includes an analysis of previous and current research achievements, presents findings in a comprehensive way, and suggests future direction in crop improvement using biotechnological tools.

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Improved Micropropagation of Plantlets from Nodal Explants of Anchote (Coccinia abyssinica)—A Calcium- and Protein-rich Tuber

Cited by 6 publications

References 10 publications

Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants

Enhanced Plantlet Regeneration in Two Cacao (Theobroma cacao) Clones from Immature Inflorescence Explants

In Vitro Culture of Heuchera villosa ‘Caramel’

Coccinia abyssinica (Lam.) Cogn. (Anchote) Biology, Productivity, and Prospects of Genetic Improvement Using Biotechnological Tools

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