Improved micropropagation and in vitro fruiting of Morus indica L. (K-2 cultivar)

Abstract

A rapid economically viable micropropagation protocol has been developed in the present work for Morus indica L. (K-2 cultivar) utilizing the readily available nodal explants. Explants were established on different plant growth regulators (PGRs) either individually or in combinations. MS medium containing 1 mg L−1 Kinetin (Kin) showed the best shoot multiplication with 4.8 ± 0.23 cm average shoot length and 6.5 ± 0.03 number of internodes. Regenerated shoots were elongated in MS medium supplemented with 1.5 mg L−1 gibberellic acid (GA3). Elongated shoots cultured in full-strength MS medium supplemented with 1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) for one week and then cultured in half-strength MS proved to be more effective in rooting compared to other PGRs in significantly shorter duration. Micropropagated plants transferred to soil fortified with the quarter-strength of MS salts along with humidity regulation process showed 89% survival frequency. In vitro flowering in the regenerated shoots was also observed in the MS medium supplemented with (1.5 mg L−1) Kin and carbon source replaced by commercial sugar cubes. This method can be effectively used for in vitro culture of M. indica in commercial scale owing to its enhanced quality and reduced time frame.

Optimization of in vitro culture conditions affecting propagation of mulberry plant

“…Also, a similar situation was also noticed by Santamaria (Pattnaik et al, 1996). On the other hand, Gogoi et al (Gogoi & Borua, 2017) reported that supplementation of the MS medium with 1.5 mg/l of gibberelic acid (GA 3 ) improved elongated shoots of Morus indica L. (K2-cultivar). Regarding rooting stage, it is well known that auxin regulates have various aspects of plant growth and development by affecting numerous processes including cell division, cell enlargement, and differentiation (Srivastava, 2002;Woodward & Bartel, 2005).…”

In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

“…A high percentage of seed germination (90%) has been reported on sterile moistened filter paper or cotton in Petri dishes or test tubes in various crop species, including snake gourd (Pillai et al, 2008;Devendra et al, 2008 and; Brassica oleracea (Finch-Savage et al, 2006) and Triticum aestivum (Lehner et al, 2006) Ophiorrhiza mungos (Namdeo et al, 2012), Ipomoea sepiaria (Cheruvathur et al, 2015). In the present study, the higher regeneration efficiency was observed in leaf explants excised from 7-10 day-old seedlings (Gogoi et al, 2017), whereas in earlier reports cotyledon explants used were older than 3-4 days (Prem Kumar et al, 2015). Therefore, seeds were germinated on sterile moistened cotton and used for all the experiments in the present study.…”

Variation of axillary growth as respond of Morus spp. micropropagation using various concentration of Indonesian local solid substance

“…The explant obtained in September was more susceptible to bacterial infection because of the high rate of rainfall (Vijayan et al 2011). Parental age also affects the contamination rate (Gogoi et al 2017). The older explant source was more susceptible to contamination.…”

“…The amount of light also supports flowering. The light plays a role as a sucrose regulator to induce florigen (Gogoi et al 2017).…”

“…There are a lot of different species in Indonesia and each species needs different environments to grow. It depends on plant's cultivar, genotype, and environment (Gogoi et al 2017). Bogor, West Java is one of the places providing the growth of Morus spp.. Morus bombycis var.…”