Improved lignocellulose saccharification of a <i>Miscanthus</i> reddish stem mutant induced by heavy‐ion irradiation

Wang, Congpeng; Guo, He; Meng, Jie; Wang, Shumin; Kong, Yingzhen; Jiang, Jianxiong; Hu, Ruibo; Zhou, Guangsheng

doi:10.1111/gcbb.12748

Cited by 7 publications

(5 citation statements)

References 64 publications

(108 reference statements)

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“…The Klason method is a classic technique for determining lignocellulose, but it overestimates the true lignin value of raw materials. The application of this method is limited because it cannot determine soluble fiber and remove farinaceous substance (Hatfield et al, 1994;Wang et al, 2020). New and state-of-the art technologies, such as near-infrared spectroscopy and nuclear magnetic resonance, have been widely used in determining lignocellulose.…”

Section: Introductionmentioning

confidence: 99%

Natural Variation of Lignocellulosic Components in Miscanthus Biomass in China

Cheng

Han

et al. 2020

Front. Chem.

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Section: Introductionmentioning

confidence: 99%

Natural Variation of Lignocellulosic Components in Miscanthus Biomass in China

Cheng

Han

et al. 2020

Front. Chem.

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“…Inducing random mutations using radiation is an alternative approach for creating variation that circumvents the need of advanced transformation techniques [ 251 ]. The downside of such an approach is the large number of mutated plants that need to be screened, as most mutations will result in no or undesired effects.…”

Section: Breeding For Improved Feedstock Qualitymentioning

confidence: 99%

“…The downside of such an approach is the large number of mutated plants that need to be screened, as most mutations will result in no or undesired effects. Wang et al [ 251 ] used heavy-ion irradiation and were able to identify a promising mutant with lower lignin content and higher saccharification efficiency without a reduction in yield. Although the genes that were altered could not be identified, they mention it was likely, given the red stem phenotype that several lignin, flavonoid and anthocyanin pathways were affected.…”

Section: Breeding For Improved Feedstock Qualitymentioning

confidence: 99%

Breeding Targets to Improve Biomass Quality in Miscanthus

et al. 2021

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Lignocellulosic crops are attractive bioresources for energy and chemicals production within a sustainable, carbon circular society. Miscanthus is one of the perennial grasses that exhibits great potential as a dedicated feedstock for conversion to biobased products in integrated biorefineries. The current biorefinery strategies are primarily focused on polysaccharide valorization and require severe pretreatments to overcome the lignin barrier. The need for such pretreatments represents an economic burden and impacts the overall sustainability of the biorefinery. Hence, increasing its efficiency has been a topic of great interest. Inversely, though pretreatment will remain an essential step, there is room to reduce its severity by optimizing the biomass composition rendering it more exploitable. Extensive studies have examined the miscanthus cell wall structures in great detail, and pinpointed those components that affect biomass digestibility under various pretreatments. Although lignin content has been identified as the most important factor limiting cell wall deconstruction, the effect of polysaccharides and interaction between the different constituents play an important role as well. The natural variation that is available within different miscanthus species and increased understanding of biosynthetic cell wall pathways have specified the potential to create novel accessions with improved digestibility through breeding or genetic modification. This review discusses the contribution of the main cell wall components on biomass degradation in relation to hydrothermal, dilute acid and alkaline pretreatments. Furthermore, traits worth advancing through breeding will be discussed in light of past, present and future breeding efforts.

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“…Similar strategy was also tried by Wu et al [ 91 ]. In another study, miscanthus mutant was also constructed via heavy ion mutagenesis, showing lower lignin content, higher cellulose content and higher saccharification efficiency compared with the parental plant [ 92 ]. In breeding process, innovations such as novel mutagenesis technology, marker-assisted selection technology and genome-editing technology will speed up breeding of modified cell walls of crops or energy crops varieties [ 92 – 94 ].…”

Section: Introductionmentioning

confidence: 99%

Microbial tolerance engineering for boosting lactic acid production from lignocellulose

Shan

Yan

et al. 2023

Biotechnol Biofuels

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Lignocellulosic biomass is an attractive non-food feedstock for lactic acid production via microbial conversion due to its abundance and low-price, which can alleviate the conflict with food supplies. However, a variety of inhibitors derived from the biomass pretreatment processes repress microbial growth, decrease feedstock conversion efficiency and increase lactic acid production costs. Microbial tolerance engineering strategies accelerate the conversion of carbohydrates by improving microbial tolerance to toxic inhibitors using pretreated lignocellulose hydrolysate as a feedstock. This review presents the recent significant progress in microbial tolerance engineering to develop robust microbial cell factories with inhibitor tolerance and their application for cellulosic lactic acid production. Moreover, microbial tolerance engineering crosslinking other efficient breeding tools and novel approaches are also deeply discussed, aiming to providing a practical guide for economically viable production of cellulosic lactic acid.

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Improved lignocellulose saccharification of a Miscanthus reddish stem mutant induced by heavy‐ion irradiation

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 7 publications

References 64 publications

Natural Variation of Lignocellulosic Components in Miscanthus Biomass in China

Natural Variation of Lignocellulosic Components in Miscanthus Biomass in China

Breeding Targets to Improve Biomass Quality in Miscanthus

Microbial tolerance engineering for boosting lactic acid production from lignocellulose

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