Improved Islet Survival and Funtion With Rat Endothelial Cells In Vitro Co-Culture

Song, Huanjin; Xue, Wujun; Li, Y.; Tian, Xin; Song, Yong; Ding, Xiaoming; Xiong, Feng; Tian, Peng; Li, Z.-L.

doi:10.1016/j.transproceed.2009.09.071

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…We performed in vitro studies using a co-culture system to confirm our in vivo findings. Song et al (24) reported that islet function was improved when rat islets were co-cultured with endothelial cells. Of note, we found that MIN6 cells displayed marked improvements in glucose tolerance when they were co-cultured with MS-1 cells treated with Ang(1-7) compared with MIN6 cells co-cultured with untreated MS-1 cells, and this effect was attenuated by A779.…”

Section: Discussionmentioning

confidence: 99%

The angiotensin-converting enzyme 2/angiotensin (1–7)/Mas axis protects the function of pancreatic β cells by improving the function of islet microvascular endothelial cells

Wang

et al. 2014

International Journal of Molecular Medicine

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In the diabetic state, the local rennin-angiotensin system (RAS) is activated in the pancreas, and is strongly associated with islet dysfunction. The angiotensin-converting enzyme 2 (ACE2)/angiotensin (1-7) [Ang(1-7)]/Mas axis is a protective, negative regulator of the classical renin-angiotensin system. In this study, we assessed the role of the ACE2/Ang(1‑7)/Mas axis in pancreatic β cell survival and function. ACE2 knockout and wild-type mice were fed a high-fat diet for 16 weeks. We then performed terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assays, and determined the expression levels of interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in the pancreatic islets. The effects of Ang(1-7) or Mas receptor silencing on endothelial function were assessed in MS-1 cells. MIN6 cells were then co-cultured with the MS-1 cells to evaluate the effects of ACE2 on insulin secretion. The ACE2 knockout mice were more susceptible than the wild-type mice to high-fat diet-induced β cell dysfunction. The TUNEL-positive area of the pancreatic islets and the expression levels of IL-1β and iNOS were markedly increased in the ACE2 knockout mice compared with their wild-type littermates. The Mas-silenced MS-1 cells were more sensitive to palmitate-induced dysfunction and apoptosis in vitro. Ang(1-7) increased the activity of the Akt/endothelial NOS/nitric oxide (NO) pathway in the MS-1 cells, protected MIN6 cells against palmitate-induced apoptosis, and improved MIN6 insulin secretory function in the co-culture system. In conclusion, this study demonstrates that the ACE2/Ang(1-7)/Mas axis is a potential target for protecting the funcion of β cells by improving the function of islet microvascular endothelial cells.

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Section: Discussionmentioning

confidence: 99%

The angiotensin-converting enzyme 2/angiotensin (1–7)/Mas axis protects the function of pancreatic β cells by improving the function of islet microvascular endothelial cells

Wang

et al. 2014

International Journal of Molecular Medicine

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“…Mainly used in drug research and development, biopharmaceutical, and other research. Through in vitro coculture of islet and vascular endothelial cells, Song et al (2009) found that the activity of islet cells and insulin release test were improved, providing a basis for the feasibility of large‐scale clinical islet transplantation, to overcome pancreatic cell shortage.…”

Section: Coculture For Antidiabetic Assaymentioning

confidence: 99%

Guidelines for the antidiabetic assay for bioactive substances in cell model

Zhang

Cao

et al. 2022

eFood

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Diabetes is a group of metabolic diseases characterized by hyperglycemia. Insulin resistance (IR) and insufficient insulin secretion are important pathophysiological basis and significant characteristics of diabetes. At present, animal is still the main model for investigation of Antidiabetic activity of bioactive substances, however, in vivo models always has the problems of long experimental cycle, complicated experiment, and high cost. Compared with the animal models, cell culture could avoid some impossible or difficult to eliminate under natural conditions, and observe the experimental results more simply, economically, quickly, and objectively. In this case, the establishment of in vitro diabetic cell model has gradually developed. Recently, cell models are more widely used in highthroughput screening, metabolism analysis, molecular pharmacology investigation, pharmacodynamics evaluation of natural product, cell function, interaction between receptors and cytokines, and so on. These guidelines will point out and discuss to help researchers to choose cell model that are truly reliable and can be successfully applied for their intended investigation of antidiabetic activity.

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“…Cells were isolated from the abdominal aorta of Wistar rats (n = 6) using an enzymatic treatment method (Song et al, 2009). SMCs were cultivated with DMEM supplemented with 10% FBS and 50 μg/mL gentamicin.…”

Section: Isolation Of Smooth Muscle Cells (Smcs)mentioning

confidence: 99%

Small-Diameter Vessels Reconstruction Using Cell Tissue-Engineering Graft Based on the Polycaprolactone

et al. 2021

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Polycaprolactone (PCL) is widely applied for the construction of small-diameter tissue-engineered vascular grafts (TEGs) due to its biomechanical properties, slow degradation, and good biocompatibility. In the present study the TEG based on a tubular scaffold seeded with smooth muscle aortic cells (SMCs) in a rat abdominal aorta replacement model was tested. Polyester tubular scaffolds were generated by thermally induced phase separation and seeded with rat SMCs. To track the implanted SMCs in vivo, cells were labeled with superparamagnetic iron oxide nanoparticles (SPIONs). Histological evaluation of the migration of autologous endothelial cells (ECs) and formation of the endothelial lining was performed 4, 8, and 12 weeks after graft interposition. TEG demonstrated a high patency rate without any complications at the end of the 12-week period. The migration of ECs into the lumen of the implanted TEG and formation of the cell monolayer were already present at 4 weeks, as confirmed by histological analysis. The architecture of both neointima and neoadventitia were similar to those of the native vessel. SPION-labeled SMCs were detected throughout the TEG, indicating the role of these cells in the endothelization of scaffolds. The SMC-seeded scaffolds demonstrated improved patency and biointegrative properties when compared to the acellular grafts.

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Improved Islet Survival and Funtion With Rat Endothelial Cells In Vitro Co-Culture

Cited by 7 publications

References 15 publications

The angiotensin-converting enzyme 2/angiotensin (1–7)/Mas axis protects the function of pancreatic β cells by improving the function of islet microvascular endothelial cells

The angiotensin-converting enzyme 2/angiotensin (1–7)/Mas axis protects the function of pancreatic β cells by improving the function of islet microvascular endothelial cells

Guidelines for the antidiabetic assay for bioactive substances in cell model

Small-Diameter Vessels Reconstruction Using Cell Tissue-Engineering Graft Based on the Polycaprolactone

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