Improved Identification of Yeast Species Directly from Positive Blood Culture Media by Combining Sepsityper Specimen Processing and Microflex Analysis with the Matrix-Assisted Laser Desorption Ionization Biotyper System

Yan, Yingjun; He, Ying; Maier, Τ.; Quinn, Criziel D.; Shi, Gongyi; Li, Haijing; Stratton, Charles W.; Kostrzewa, Markus; Tang, Yi‐Wei

doi:10.1128/jcm.00339-11

Cited by 105 publications

(93 citation statements)

References 32 publications

(45 reference statements)

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“…While some authors have had very low success rates with fungi (Buchan et al, 2012;Idelevich et al, 2014), others have reported excellent levels of identification with different sample processing methods (Ferroni et al, 2010(Ferroni et al, , 2011Idelevich et al, 2014;Lavergne et al, 2013;Marinach-Patrice et al, 2010;Yan et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

Kouadio-Yapo¹,

Hasseine²,

Delaunay³

et al. 2018

Afr. J. Microbiol. Res.

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This study aimed to identify yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry. This was a 5-month prospective study (February to June, 2015) carried out on bottles of blood cultures from in-patients at the Hôpital de l'ARCHET at the CHU, Nice. Positive blood culture broth was analysed by MALDI-TOF mass spectrometry using Sodium Dodecyl Sulphate (SDS) as the lysis buffer. Out of 64 samples, 51 (79.6%) were identified by MALDI-TOF mass spectrometry at the species level and 12 strains (18.7%) at the genus level. The isolated yeasts fell into six species: Candida albicans 37.2%, Candida glabrata 31.4%, Candida parapsilosis 15.7%, Candida tropicalis 5.9%, Candida krusei 5.9%, and Candida lusitaniae 3.9%. In comparison, 95.3% of species were identified from cultured colonies, the main ones being C. albicans (37.1%), C. glabrata (30.7%) and C. parapsilosis (16.1%). Identification of yeasts from blood culture bottles by MALDI-TOF mass spectrometry is a fast, reliable technique. However, analysis of colonies remains the best technique for identification and for antifungal imaging in order to refer the patient to the most appropriate therapy.

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Section: Discussionmentioning

confidence: 99%

“…Several studies have been carried out on direct identification of yeasts from positive blood culture bottles using various protocols with varying results (Buchan et al, 2012;Drancourt, 2010;Ferreira et al, 2011;Idelevich et al, 2014;Lavergne et al, 2013;Marinach-Patrice et al, 2010;Yan et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

Kouadio-Yapo¹,

Hasseine²,

Delaunay³

et al. 2018

Afr. J. Microbiol. Res.

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“…Para la identificación directa a partir de hemocultivos positivos en sistemas automatizados hay varias referencias que muestran la utilidad de utilizar MALDI-TOF MS 6,24,[45][46][47][48][49] . Como se explicó anteriormente, para obtener espectros a partir de los hemocultivos, es necesario un proceso previo de extracción de los componentes de la sangre y del medio de cultivo.…”

Section: En La Identificación De Bacterias Y Levaduras Directamente Dunclassified

Identificación bacteriana basada en el espectro de masas de proteínas: Una nueva mirada a la microbiología del siglo XXI

García

Allende

Legarraga

et al. 2012

Rev. chil. infectol.

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Bacterial identification based on protein mass spectrometry:A new insight at the microbiology of the 21 st centuryBacterial identification is important for the proper treatment of infected patients hospitalized with serious infections especially in critical care units. Identification by conventional methods used in microbiology laboratories takes at least 16 hours since a culture is positive. The introduction of mass spectrometry, specifically MALDI-TOF MS (matrix-assisted laser desorption/ ionization time-of-flight mass spectrometer) in the microbiology laboratory could mean a radical change in the identification accuracy, turn around time (6 minutes per bacteria) and cost (about 5 times cheaper than conventional identification). Since its introduction in clinical microbiology laboratories in 2008, many reports about its usefulness in identifying microorganisms from colonies, as well as directly from positive blood cultures and urine samples have been published. This review describes MALDI-TOF MS methodology, its identification performance for bacteria (aerobic and anaerobic), mycobacterium and yeasts, its future applications in microbiology and its main disadvantages.Key words: Bacterial identification, mass spectrometry. Palabras clave: Identificación bacteriana, espectrometría de masas, MALDI-TOF MS. Pontificia Universidad Católicade Chile, Santiago. Facultad de MedicinaDepartamento de Laboratorios Clínicos Laboratorio deMicrobiología (PG). Servicio de Laboratorios Clínicos (MH).Departamento de Laboratorios Clínicos. Laboratorio de Bioquímica, SecciónEspectrometría de Masas (FA, SS). Departamento de MedicinaInterna (PL). Conflictos de interés: no hay. Recibido: 19 de julio de 2011Aceptado: 26 de noviembre de 2011 Correspondencia a:Sandra Solari Gajardo, ssolari@med.puc.cl IntroducciónT radicionalmente la identificación bacteriana se ha basado en la utilización de métodos fenotípicos que incluyen las características morfológicas y tintoriales que presentan los microorganismos en distintos medios de cultivos, así como las reacciones bioquímicas propias de cada especie 1 . Sin embargo, esto conlleva una carga laboral y consumo de tiempo importante para el laboratorio, puesto que la identificación bacteriana puede tomar desde horas e incluso hasta días, sobre todo para aquellos microorganismos fastidiosos, lo cual sin duda, impacta directamente en el pronóstico del enfermo, pues es sabido que mientras más precoz y apropiado es el antimicrobiano, menor es la mortalidad del paciente, en especial para aquellos que están graves [2][3][4] . Es así como algunos reportes demuestran un incremento en la mortalidad de 7% por hora cuando existe un retraso en el inicio de antimicrobianos para aquellas personas que están hipotensas y en estado séptico 5 . Ante esta situación se ha generalizado el uso precoz de antimicrobianos de amplio espectro dado la dificultad para obtener un diagnóstico rápido de las bacterias involucradas, lo que ha llevado al uso, muchas veces, de tratamientos indebidos, con la consiguiente preocupació...

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“…Для амплификации генов 16S рРНК, blaZ, hla использовали олигонуклеотидные праймеры 16Sup, 16Slow [17], blaZ-F, blaZ-R, hlA1, hlA2 соответственно, последовательность праймеров представлена в таблице 1. Амплификацию проводили в 25 мкл реакционной смеси, содержащей 66 мМ Tris-HCl (рН 9,0), 16 Включённые в исследование штаммы S. aureus были протестированы на наличие генов hla и blaZ, кодирующих a-гемолизин и b-лактамазу соответственно. Наличие или отсутствие генов hla и blaZ было установлено по результатам амплификации со специфическими праймерами.…”

Section: методикаunclassified

“…Впервые использование масс-спектрометрии для идентификации бактерий было предложено в 1975 году [9], сейчас метод прямого MALDI -масс-спектрометрического профилирования используется многими клиническими и научными лабораториями мира для решения задач по исследованию клеточных микроорганизмов [10][11][12][13][14][15][16]. Данный подход основан на накоплении MALDI масс-спектров грубого лизата клеток исследуемого микроорганизма и последующим сопоставлении их с аналогичными масс-спектрами, хранящимися в базах данных или между собой.…”

Section: Introductionunclassified

Strain differentiation of staphylococcus aureus by means of direct maldi tof mass spectrometry profiling

et al. 2012

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Staphylococcus aureus - one of the most interesting for clinical studies of microbial species with extensive strain diversity, primarily due to the variability of virulence factors and pathogenicity. The aim of this study was approbation of a method for the rapid strain differentiation of S. aureus on the basis of bacterial cell direct protein profiling approach by means of MALDI TOF MS. Beta-lactamase and alpha-hemolysin productions, cording by the blaZ and hla genes, respectively, were selected as markers for the strain differentiation. Mathematical analysis of MALDI mass spectra from 53 isolates allowed the construction of two independent classification models that can differentiate the strains on the presence/absence of blaZ or hla genes. A number of the most significant peaks (masses), which can be considered as markers of the strain differences in S. aureus, were identified using a statistical contribution of each mass peak in the models. These diagnostic models differ the sensitivity and the specificity, which were 97.5% and 82.5% for the classification of strains on the basis of beta-lactamase production, and 90.0% and 88.7% by the presence of alpha-hemolysin.

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Improved Identification of Yeast Species Directly from Positive Blood Culture Media by Combining Sepsityper Specimen Processing and Microflex Analysis with the Matrix-Assisted Laser Desorption Ionization Biotyper System

Cited by 105 publications

References 32 publications

Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

Direct identification of yeasts from blood culture by MALDI-TOF mass spectrometry

Identificación bacteriana basada en el espectro de masas de proteínas: Una nueva mirada a la microbiología del siglo XXI

Strain differentiation of staphylococcus aureus by means of direct maldi tof mass spectrometry profiling

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