Improved high-performance liquid chromatographic method for the analysis of insulins and related compounds

Development of RP-HPLC for analysis of human insulin

“…Thus several analytical techniques such as electrophoresis, spectroscopy, chromatography, thermal analysis, immuno assays and bioassays may be required to completely characterize a protein and examine its degradation profiles 4 . Since 1978, several RP-HPLC systems for analysis of insulin have been described [2][3][4][5][6][7][8][9][10][11][12] . Most of these RP-HPLC (n = 3).…”

“…However, some of these methods ratios of measured concentration to known added suffer to resolve adequately insulin from its desamidated concentration were calculated in each case. form, because of inadequate selectivity or efficiency [5][6][7][8] . Some are time consuming and retention time of insulin-Twenty successive injections of 0.01 M HCl (blank) were related peak is too long 3,9,10 .…”

“…The assay has been validated and documented. The assay has some benefits when compared to previously published HPLC assays 6,8,12 . Of primary importance, this respectively.…”