Improved high-performance liquid chromatographic analysis of methylated amino acids and related compounds and its application to the analysis of samples of polluted healthy and damaged Picea needles without extract purification

Villanueva, Victor R.; Mardon, M.; Goff, M.-T. Le; Moncelon, F.

doi:10.1016/s0021-9673(01)94208-6

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…according to the method described by Villanueva et al (1987) with minor modification. The automated analytical system was equipped with a solvent selection system (LV-980-03), 2 h.p.l.c.…”

Section: Measurement Of Mechanical Responsesmentioning

confidence: 99%

“…Determination of L-arginine, L-NMMA, ADMA and SDMA Contents of L-arginine, L-NMMA, ADMA and SDMA in the regenerated and control ECs were determined by means of automated high-performance liquid chromatography (h.p.l.c.) according to the method described by Villanueva et al (1987) with minor modification. The automated analytical system was equipped with a solvent selection system (LV-980-03), 2 h.p.l.c.…”

Section: Measurement Of Mechanical Responsesmentioning

confidence: 99%

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Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells

Azuma

Sato

Hamasaki

et al. 1995

British J Pharmacology

124

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1 We examined regeneration of endothelial cells (ECs), neointima formation, decreased endotheliumdependent relaxation (EDR) and changes in the contents of L-arginine, NG-monomethyl-L-arginine (L-NMMA), asymmetrical NG, N0-dimethylarginine (ADMA) and symmetrical NG,NG-dimethylarginine (SDMA) in the regnerated ECs, 6 weeks after balloon denudation of the rabbit carotid artery. 2 Regeneration of ECs was completed in 6 weeks and a significant neointima formation accompanied by the decreased EDR was observed. 3 L-NMMA and ADMA contents in the regenerated ECs (23.5 + 4.3 and 21.2 + 2.0 pmol mg-' DNA, respectively) were significantly (P <0.05 and P <0.01) higher than those in the control ECs (8.8 + 3.0 and 7.4 + 1.9 pmol mg'l DNA, respectively), whereas L-arginine was significantly (P < 0.005) decreased in the regenerated ECs (31,470+1,050 pmol mg-' DNA) as compared to that in the control ECs (47,870+ 1,890 pmol mg-' DNA). SDMA content was below the assay limits. 4 L-NMMA and ADMA, but not SDMA, inhibited the EDR induced by acetylcholine in a concentration-dependent manner. The inhibition with L-NMMA and ADMA was prevented by an addition of L-arginine, but not by D-arginine. 5 These results suggest that the accumulation of endogenous inhibitors for nitric oxide synthesis and decreased L-arginine content are associated with decreased NO production/release from regenerated ECs and neointima formation. Keywords: Neointima formation; endogenous inhibitors for NO synthesis; balloon denudation; decreased endotheliumdependent relaxation; EDRF/NO Introduction MethodsAs proposed by Ross & Glomset (1976a,b) and Ross (1986), denudation and nondenudation intimal injuries may play an important role in the development of atherosclerotic vascular disease (Ip et al., 1990). We have reported that restoration of the endothelium after balloon denudation of the rabbit carotid artery was completed in 6 weeks and that regenerated ECs were less capable of producing/releasing the endothelium-derived relaxing factor (EDRF/nitric oxide: NO) (Azuma et al., 1990;Niimi et al., 1994). EDRF/NO inhibits platelet adhesion and aggregation (Azuma et al., 1986;Radomski et al., 1987), which is considered as an initiating event for neointima formation (Ross, 1986;Schwartz et al., 1981). It has also been demonstrated that NO inhibits smooth muscle cell proliferation (Garg & Hassid, 1989). These findings when considered together suggest that the neointima formation after endothelial removal might be caused at least partly through impairment of the ability of ECs to produce/release EDRF/NO. However, the mechanism by which the production/release of EDRF/NO is reduced remains unclear. Recently, Vallance et al. (1992) have obtained evidence NG-monomethyl-L-arginine (L-NMMA) and asymmetrical NG,NG-dimethyl arginine (ADMA) play a role as endogenous inhibitors of NO synthesis. The present experiments were, therefore, designed to determine the regeneration of ECs, the neointima formation, the decreased EDR and the changes in content of L-arginine, L-NMMA, ADMA an...

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“…according to the method described by Villanueva et al (1987) with minor modification. The automated analytical system was equipped with a solvent selection system (LV-980-03), 2 h.p.l.c.…”

Section: Measurement Of Mechanical Responsesmentioning

confidence: 99%

Section: Measurement Of Mechanical Responsesmentioning

confidence: 99%

Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells

Azuma

Sato

Hamasaki

et al. 1995

British J Pharmacology

124

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“…1 Methylated products of histidine, lysine, and arginine were detected in plant leaves of Picea using high-performance liquid chromatography (HPLC). 2 Further, methylated amino acids were reported in insects such as silkworm and Bombyx mori, 3 fungi samples, eg, Saccharomyces cerevisiae (spices of yeast), 4 nerve tissue, and glands of endocrine of different animals, 5 human platelets, 6 human urine, 7 human serum, 8 human plasma, 9 human adult teeth, 10 biological tissues, 11 and Torpedo californica electric organ. 12 N-methyl Alanine was identified in human fecal sample.…”

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confidence: 99%

ESI‐MS/MS analysis of protonated N‐methyl amino acids and their immonium ions

et al. 2019

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Methylation is one of the important posttranslational modifications of biological systems. At the metabolite level, the methylation process is expected to convert bioactive compounds such as amino acids, fatty acids, lipids, sugars, and other organic acids into their methylated forms. A few of the methylated amino acids are identified and have been proved as potential biomarkers for several metabolic disorders by using mass spectrometry-based metabolomics workstation. As it is possible to encounter all the N-methyl forms of the proteinogenic amino acids in plant/biological systems, it is essential to have analytical data of all N-methyl amino acids for their detection and identification. In earlier studies, we have reported the ESI-MS/MS data of all methylated proteinogenic amino acids, except that of mono-N-methyl amino acids. In this study, the N-methyl amino acids of all the amino acids (1-21; including one isomeric pair) were synthesized and characterized by ESI-MS/MS, LC/MS/MS, and HRMS. These data could be useful for detection and identification of N-methyl amino acids in biological systems for future metabolomics studies. The MS/MS spectra of [M + H] + ions of most N-methyl amino acids showed respective immonium ions by the loss of (H 2 O, CO). The other most common product ions detected were [MH-(NH 2 CH 3 ] + , [MH-(RH)] + (where R = side chain group) ions, and the selective structure indicative product ions due to side chain and N-methyl group. The isomeric/isobaric N-methyl amino acids could easily be differentiated by their distinct MS/MS spectra. Further, the MS/MS of immonium ions inferred side chain structure and methyl group on α-nitrogen of the N-methyl amino acids. KEYWORDS immonium ions, methylation, MS/MS, N-methyl amino acids

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Effects of Pentamidine on Polyamine Level and Biosynthesis in Wild-Type, Pentamidine-Treated, and Pentamidine-ResistantLeishmania

Basselin

Badet‐Denisot

Lawrence

et al. 1997

Experimental Parasitology

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Improved high-performance liquid chromatographic analysis of methylated amino acids and related compounds and its application to the analysis of samples of polluted healthy and damaged Picea needles without extract purification

Cited by 8 publications

References 10 publications

Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells

Accumulation of endogenous inhibitors for nitric oxide synthesis and decreased content of L‐arginine in regenerated endothelial cells

ESI‐MS/MS analysis of protonated N‐methyl amino acids and their immonium ions

Effects of Pentamidine on Polyamine Level and Biosynthesis in Wild-Type, Pentamidine-Treated, and Pentamidine-ResistantLeishmania

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