Improved Hepatic Regeneration With Reduced Injury by Redox Factor-1 in a Rat Small-Sized Liver Transplant Model

Guo, Lei; Haga, Sanae; Enosawa, Shin; Naruse, Katsutoshi; Harihara, Yasushi; Sugawara, Yasuhiko; Irani, Kaikobad; Makuuchi, Masatoshi; Ozaki, Michitaka

doi:10.1111/j.1600-6143.2004.00444.x

Cited by 24 publications

(7 citation statements)

References 31 publications

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“…Regarding both TUNEL results and enzyme levels, single direct application of Epo into the portal vein appears to be superior to pretreatment with consecutive subcutaneous injections. Whereas apoptotic activity was high in the early phase 6 h after I/R injury (62.10% apoptosis rate in the control group) apoptotic activity decreased extensively within the next hours leaving only few apoptotic cells 12 h after reperfusion and hardly any apoptosis at all after 24 h. This phenomenon has been described by various authors suggesting a rapid clearance and processing of apoptotic cells within the damaged organ [9,[17][18][19]32] .…”

Section: Discussionmentioning

confidence: 70%

“…Sepodes et al [16] recently published a study showing protective capacities of Epo in liver ischemia. Numerous agents have been investigated extensively in the past as potential protectors of I/R injury yet clinical relevance and applicability have been limited [17][18][19] . The application of steroids has been shown to reduce cellular damage although side effects such as immunological depression and extensive destabilization of glucose metabolism can be a problem [20] .…”

Section: Introductionmentioning

confidence: 99%

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Erythropoietin Reduces Ischemia-Reperfusion Injury in the Rat Liver

et al. 2007

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Background: Human recombinant erythropoietin (Epo) has recently been shown to be a potent protector of ischemic damage in various organ systems. A significant reduction of stroke injury following cerebral ischemia has been postulated as well as improved cardiomyocyte function after myocardial infarction in tissue pretreated with Epo. It was the aim of this study to evaluate the effects of Epo in liver ischemia. Material and Methods: Rats were subjected to 45 min of warm hepatic ischemia. Animals were either pretreated with 1,000 IU of Epo in three doses or received 1,000 IU into the portal vein 30 min before ischemia. Control animals received saline at the same time points before ischemia. Animals were than sacrificed 6, 12, 24, 48 h and 7 days after surgery and transaminases were measured. Liver specimens were evaluated regarding apoptosis, necrosis and regeneration capacity. Results: Apoptosis rates were dramatically reduced in animals pretreated with Epo while mRNA of tumor necrosis factor-α and STAT-3 were upregulated in all groups. Intraportal venous injection displayed superiority to subcutaneous preconditioning. Transaminases were significantly reduced among the Epo-treated animals after 6 and 12 h. Conclusion: Our data suggests a protective effect of Epo in warm hepatic ischemia and reperfusion injury in the rat.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Erythropoietin Reduces Ischemia-Reperfusion Injury in the Rat Liver

et al. 2007

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“…Our study revealed that ablation of p66 shc suppressed cellular apoptosis as well as ROS production after H/R through up-regulation of MnSOD and Ref-1. Anti-oxidant molecules such as MnSOD and Ref-1 are known to suppress H/R-induced apoptosis [4,13,14,[37][38][39]. Accordingly, they may possess direct or indirect anti-apoptotic functions other than anti-oxidation.…”

Section: Discussionmentioning

confidence: 98%

Preventing hypoxia/reoxygenation damage to hepatocytes by p66shc ablation: Up-regulation of anti-oxidant and anti-apoptotic proteins

Haga

Terui

Fukai

et al. 2008

Journal of Hepatology

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“…As we showed in figure 2, the spectrum of grafts applied is very wide, from the slightly reduced 70% graft [8,32] to the really challenging 20% partial liver transplant [68,71]. A liver graft is small for size when the GRWR or the graft/standard liver volume is <0.8-1.0 or 40-50%, respectively [64,72].…”

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Improved Hepatic Regeneration With Reduced Injury by Redox Factor-1 in a Rat Small-Sized Liver Transplant Model

Cited by 24 publications

References 31 publications

Erythropoietin Reduces Ischemia-Reperfusion Injury in the Rat Liver

Erythropoietin Reduces Ischemia-Reperfusion Injury in the Rat Liver

Preventing hypoxia/reoxygenation damage to hepatocytes by p66shc ablation: Up-regulation of anti-oxidant and anti-apoptotic proteins

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

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