Improved fish species identification by use of lab-on-a-chip technology

Application of polymerase chain reaction–restriction fragment length polymorphism analysis and lab‐on‐a‐chip capillary electrophoresis for the specific identification of game and domestic meats

“…Using the DNA100 LabChip, up to 12 samples can be tested in a single run, usually taking 1–2 h from start to finish, including post‐analysis. Furthermore, manipulation of the plastic chips is easier and less harmful compared to conventional gels which require handling during the DNA staining and detection stages 15, 16. Owing to its advantages, the lab‐on‐a‐chip system can provide ease of standardisation of DNA profiling between laboratories and it seems advantageous for routine species identification in inspection programmes destined to enforce labelling regulation of meat products.…”

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

“…Some methods include the use of PCR together with the restriction fragment length polymorphism (RFLP) [9], forensic information nucleotide sequencing (FINS) [10], polymorphism of the length of the amplified fragment (AFLP) [11], or single-strand conformational polymorphism (SSCP) [12,13]. These techniques have been applied to the identification of numerous species of fish and seafood, including gadoids [14], flatfish [15,16], salmonids [11,104], scombroids [105,106], sardines and anchovies [107,108], eels [109], and mollusks [110,111]. More recently, a study based on the use of pyrosequencing as a rapid fish tool for species identification has been published [101].…”

DNA‐Based Methods for the Identification of Commercial Fish and Seafood Species

“…Some methods include the use of PCR along with restriction fragment length polymorphism (RFLP), forensically informative nucleotide sequencing (FINS), amplified fragment length polymorphism (AFLP), or single‐stranded conformational polymorphism (SSCP). The aforementioned techniques have been applied to the identification of numerous species of fish and seafood, including gadoids (Akasaki and others 2006; Moran and Garcia‐Vazquez 2006), flatfish (Sanjuan and Comesana 2002; Comesana and others 2003), salmonids (Dooley and others 2005a; Zhang and Cai 2006), scombroids (Hsieh and others 2007; Lin and Hwang 2007), sardines and anchovies (Jerome and others 2003; Santaclara and others 2006), eels (Lin and others 2002), mollusks (Rego and others 2002; Klinbunga and others 2003), and many more.…”

“…Although sequencing has proven to be the most direct and reliable way to obtain information from PCR fragments, it is also time consuming and expensive, making it impractical for routine use in many laboratories (Lockley and Bardsley 2000; Chapela and others 2002; Dooley and others 2005a). Additionally, sequencing is not appropriate for the analysis of samples containing multiple species (Lenstra 2003).…”

“…Lab‐on‐a‐chip capillary electrophoresis A recently investigated development in PCR‐RFLP has been the replacement of the gel electrophoresis step with microfluidic, lab‐on‐a‐chip technology, which utilizes CE to analyze DNA fragments (Dooley and others 2005a, 2005b). Lab‐on‐a‐chip CE is considered an improvement to the traditional PCR‐RFLP procedure because it is easy to use, and it has been reported to exhibit increased sensitivity, speed, reliability, and safety compared to gel‐based methods.…”