Application of polymerase chain reaction–restriction fragment length polymorphism analysis and lab‐on‐a‐chip capillary electrophoresis for the specific identification of game and domestic meats

Abstract

BACKGROUND: The objective of this studywas to adapt and improve previously published polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods aimed at the identification of game and domestic meats, by replacing the gel electrophoretic steps for DNA fragment analysis by a chip-based capillary electrophoresis system.

Species identification of Malayan Gaur, Kedah-Kelantan and Bali cattle using polymerase chain reaction-restricted fragment length polymorphism

“…Therefore, this study revealed the significant role that PCR-based techniques can play in species identification, and emphasizes the importance of choosing the right genes with suitable variation rates (Brown, 1983;Wolf et al, 1999). This method was successfully used to type both Malaysian wild and domestic cattle, and has previously been used in species identification of game and domestic meats (Prado et al, 2007;Fajardo et al, 2009;Wang et al, 2010;Chen et al, 2010).…”

Advances in Authenticity Testing for Meat Speciation

“…Table 14.2 summarizes the most relevant information from reported PCR-RFLP methods, from which it can be inferred that mtDNA markers are mostly targeted to differentiate domestic and game meat species. Most PCR-RFLP methods use gel More recently, chip-based capillary electrophoresis has emerged as an alternative to conventional electrophoresis for RFLP analysis for meat authentication, producing higher resolution band patterns, shorter analysis times, higher versatility, reproducibility, and suitability for automation (Ali et al, 2012a(Ali et al, , 2015aFajardo et al, 2009a;Rahman et al, 2015). …”

Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting

“…Using primers targeting a conserved region in the 12S rRNA gene, followed by restriction enzyme treatment and electrophoretic separation, closely related poultry species could be differentiated (Saini et al, 2007). A similar approach has been used to differentiate game animals such as roe deer, red deer and mouflon from domesticated ruminants (Fajardo et al, 319 2006;Fajardo et al, 2009). Use of a single restriction enzyme may be sufficient for species differentiation by PCR-RFLP .…”