2017
DOI: 10.1007/s00253-017-8420-4
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Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli

Abstract: Sulfate (SO) is an often-utilized and well-understood inorganic sulfur source in microorganism culture. Recently, another inorganic sulfur source, thiosulfate (SO), was proposed to be more advantageous in microbial growth and biotechnological applications. Although its assimilation pathway is known to depend on O-acetyl-L-serine sulfhydrylase B (CysM in Escherichia coli), its metabolism has not been extensively investigated. Therefore, we aimed to explore another yet-unidentified CysM-independent thiosulfate a… Show more

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Cited by 35 publications
(29 citation statements)
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“…The energy efficiency of Cys synthesis from thiosulfate is better than that from sulfate because of the lower number of synthesis steps in the cytoplasm (2,4). Therefore, thiosulfate is considered a better sulfur source than sulfate in E. coli (7)(8)(9), and in the yeast Saccharomyces cerevisiae (10). Here, by performing growth tests of an E. coli strain lacking an unknown functional gene, yeeE, we have demonstrated that bacterial YeeE imports thiosulfate as a sulfur source.…”
Section: Introductionmentioning
confidence: 90%
“…The energy efficiency of Cys synthesis from thiosulfate is better than that from sulfate because of the lower number of synthesis steps in the cytoplasm (2,4). Therefore, thiosulfate is considered a better sulfur source than sulfate in E. coli (7)(8)(9), and in the yeast Saccharomyces cerevisiae (10). Here, by performing growth tests of an E. coli strain lacking an unknown functional gene, yeeE, we have demonstrated that bacterial YeeE imports thiosulfate as a sulfur source.…”
Section: Introductionmentioning
confidence: 90%
“…Sulfur metabolomics was performed by the Sulfur Index service in Japan (http://www.euglena.jp/sulfurindex/; Euglena Co. Ltd., Tokyo, Japan). The sulfur index uses a technology where metabolites are detected as S-bimanyl derivatives by LC-MS/MS (Shimadzu (Nexera UHPLC system with on-line LC-MS 8040, Shimadzu, Corporation, Kyoto, Japan) as described previously [15,16]. Briefly, the sulfur-containing compounds in the samples were extracted by adding methanol and converting to fluorescent derivatives with monobromobimane.…”
Section: Metabolomics and Sulfur Metabolomicsmentioning
confidence: 99%
“…S7). The physiological level of rhodanese activities is also not sufficient to support thiosulfate utilization in an E. coli cysM mutant that resumed growth with the overexpression of the E. coli rhodanese GlpE (30). Thus, any organisms with the sulfate assimilation system may use thiosulfate as a sulfur source with the help of a rhodanese.…”
Section: Discussionmentioning
confidence: 98%