Improved extraction and reversed phase‐high performance liquid chromatographic separation of flavonoids and the identification of Rosa cultivars

Abstract

Optimal extraction methods and Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) procedures were developed for the isolation and resolution of naturally occurring flavonoids and related phenolic compounds in Rosa petals. The following flavonoids and related phenolics were identified or partly identified: cyanidin 3,5-di-O-glucoside; cyanidin 3,s-di-0-glycoside (sugar moieties, glucose and arabinose); pelargonidin 3,s-di-0-glycoside; pelargonidin 3-0-glycoside; quercetin 3-0-glucoside; ellagic acid; quercetin 3-0-arabinoside; quercetin 4'-O-glucoside; kaempferol 4'-O-glucoside; kaempferol 3-0-rutinoside; kaempferol 3-0-glucoside; kaempferol3-0-arabinoside; kaempferol3-0-rhamnoside and kaempferol3-0-glycoside. Significant qualitative and quantitative differences between the above flavonoid markers allowed an objective distinction between 44 cultivars examined. The production of 'Flower Flavonoid RP-HPLC Fingerprints', which have been converted in the corresponding 'Computer Flower Flavonoid Identity Cards' via a suitable computer program, has proven to be of great value for Rosa cultivar recognition.

Identification of Isoflavones in the Roots ofPueraria lobata

“…In the present study, efficient separations of the polyphenolic extracts and of the hydrolyzed extracts of Puerariae Radix were achieved by analytical HPLC using gradient I (see Materials and Methods). Exhaustive extraction using methanol-water 3: 1 (v/v) delivers an extract containing the full low-molecular weight poly-phenolic material (16 Acid hydrolysis was achieved using established conditions (17) and, after work-up, the reaction mixture was analyzed by HPLC (gradient I, see Materials and Methods). The glycoside fraction appears less complex in the hydrolyzed extract, as two major peaks (5 and 7) and two minor peaks (1 and 8)…”

Separation procedures for naturally occurring antioxidant phytochemicals

“…Most of these methods have been developed to measure different groups of polyphenolics in a single plant, or a single or a few groups in multiple plant sources. Van Sumere et al [128] indeed developed a good method that separated nearly 50 phenolic compounds from the rose flower pedals. However, some important antioxidant polyphenols such as procyanidins, chlorogenic acid and phloretin-glycosides were not included in their method.…”

Antioxidant Polyphenols in Almond and Its Coproducts

“…packed with Nova-Pak C18 (4 µm, 60 Å) material. A gradient profile using two solvents was applied at room temperature (25), with solvent A ) 5% aqueous formic acid and solvent B ) acetonitrile/methanol (5:95, v/v) and a flow rate of 0.9 mL/min. Compounds of interest were detected on the basis of characteristic UV-vis spectra (spectral range of 220-400 nm) and retention times.…”