Improved expression of green fluorescent protein in cattle embryos produced by ICSI-mediated gene transfer with spermatozoa treated with streptolysin-O

Comparison of two methods of sperm‐ and testis‐mediated gene transfer in production of transgenic animals: A systematic review

“…In line with this, Sim et al (2013) reported an improvement in mouse sperm permeabilization immediately after receiving SLO in its condition media with 89% efficiency in generating Tg mice (Sim et al, 2013). Furthermore, Sánchez-Villalba et al (2018) established cattle sperm penetrability using SLO and assessed Tg calves through ICSI-SMGT (Sánchez-Villalba et al, 2018). In the SMGT method, SLO demonstrated an efficiency of 89% in Tg mice (Sim et al, 2013).…”

“…Furthermore, Sánchez‐Villalba et al. (2018) established cattle sperm penetrability using SLO and assessed Tg calves through ICSI‐SMGT (Sánchez‐Villalba et al., 2018). In the SMGT method, SLO demonstrated an efficiency of 89% in Tg mice (Sim et al., 2013).…”

Intracytoplasmic Sperm Injection in Cattle

“…ICSI has been effectively used for production of transgenic embryos [41][42][43][44]. Spermmediated gene transfer uses sperm to transport exogenous DNA into the oocyte during fertilization, resulting in transgenic embryos [42].…”

“…ICSI has been effectively used for production of transgenic embryos [41][42][43][44]. Spermmediated gene transfer uses sperm to transport exogenous DNA into the oocyte during fertilization, resulting in transgenic embryos [42]. In farm animals such as sheep, goats and cattle, transgenesis has been used to generate animals that express recombinant protein in milk, or to produce porcine organs for human xenotransplantation [43].…”

“…High blastocyst production was achieved in farm animal ICSI-MGT by chemical activation of oocytes using ionomycin and 6-dimethylaminopurine (DMAP) [41,43]. In addition, physical or chemical damage to the sperm membrane before microinjection improved ICSI-MGT [42].…”