Intracytoplasmic Sperm Injection in Cattle

Unnikrishnan, Veena; Kastelic, John P.; Thundathil, Jacob C.

doi:10.3390/genes12020198

Cited by 11 publications

(14 citation statements)

References 163 publications

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“…The potential benefits of ICSI for the production of livestock are apparent, but unlike the widespread use in other large animal species such as horses, its use in cattle has been limited by low success rates (Coy & Romar 2002, Hinrichs 2005, Unnikrishnan et al 2021a. In mice and humans, where in vivo matured oocytes are readily available, the use of ICSI results in similar rates of egg activation and embryo development to those obtained after IVF (Westphal et al 2003, Yoshida & Perry 2007.…”

Section: Bull Sperm and Incomplete Egg Activation After Icsimentioning

confidence: 99%

SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1

et al. 2022

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The discovery of PLCζ1 nearly twenty years ago as the primary Ca2+ oscillation inducing factor in the sperm of mammals represented a significant breakthrough in our quest to elucidate the molecules and pathways that promote egg activation during fertilization. The advent of the Intracytoplasmic Sperm Injection technique, which made fertilization possible without sperm capacitation, acrosome reaction, and gamete fusion, strengthened the research that led to the discovery of PLCζ1 and became an essential clinical tool for humans. The use of ICSI combined with the detection of PLCζ1 expression and mutations in infertile patients established the fundamental role of PLCζ1 in human fertility while leading to the discovery of novel components of the perinuclear theca, the site of the residence of PLCζ1 in sperm before fertilization. Remarkably, the more extensive use of ICSI in species other than humans and mice revealed poor success and exposed gaps in our understanding of PLCζ1 release and/or activation. Similarly, fertilization using sperm from mouse models lacking PLCζ1 has produced striking results whose true implications are yet to be determined. Nevertheless, answers to these unresolved questions will produce a complete picture of the adaptations and molecular players that mammalian species employ to ensure the success of the triggering event of embryo development that has linked generations since the beginning of times.

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Section: Bull Sperm and Incomplete Egg Activation After Icsimentioning

confidence: 99%

SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1

et al. 2022

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“…Intracytoplasmic spermatozoid injection (ICSI) is an in vitro fertilization technique when a spermatozoid is microinjected into cytoplasm of a mature oocyte (Unnikrishnan et al, 2021). This technique was first performed in 1976 using spermatozoids and oocytes of hamsters (Uehara & Yanagimachi, 1976).…”

Section: Introductionmentioning

confidence: 99%

“…In cattle breeding, coculturing of gametes is a more common method, because the excellent quality of gametes means higher percentage of embyos suitable for transplantation, as compared with the ICSI (Arias et al, 2015;Briski & Salamone, 2022;Fuentes et al, 2022). At the same time, ICSI is a reliable and effective reproductive technique, which demonstrates better fertilization using sex-sorted semen, with samples that have low mobility, from individuals with high genetic value, special traits (transgenic animals or threatened species) or obtained postmortem (Unnikrishnan et al, 2021). As with oocytes, ICSI can be useful for fertilizing gametes with changes that impede the fertilization (cryoconcerved oocytes, in vitro matured and retrieved from prepuberant females) (López-Saucedo et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Most researchers agree that in order to enhance ICSI efficacy for cattle, additional activation of oocytes is needed (Suttner et al, 2000, Unnikrishnan et al, 2021, because the normal fluctuations of calcium -associated with meiosis during fertilization -do not occur in this species of animals when ICSI method is used, which impedes the following development of embryo (Valencia et al, 2021). There are known different procedures of artificial oocyte activation, which are usually divided into three subtypes -mechanical, electrical and chemical stimuli (Nasr-Esfahan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Influence of calcium ionophore on the fertilization of bovine oocytes and their further embryonic development

Kovpak

DERKACH

et al. 2023

Regul. Mech. Biosyst.

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Intracytoplasmic spermatozoid injection (ICSI) is one of the commonest methods used in assisted reproductive technologies in human medicine. However, this procedure has low efficacy for bovines, mainly because of insufficient activation of oocytes after spermatozoid microinjection. One of the most effective methods of activating oocytes is considered to be the use of phosphorus calcium, though the optimal concentration of activator and its effect on pre-implant development of embyo are still open questions. An oocyte-cumulus complex of clinically healthy cows, retrieved from the ovaries during slaughter, matured over 22–24 h in in vitro conditions. Oocytes with visible polar body had been subjected to intracytoplasmic spermatozoid injection (ICSI), and were 15–30 min later activated in the environment with different concentrations of calcium ionophore for 15–20 min and then transferred for further cultivation in a culture medium with sodium pyruvate. The fertilization rate was identified on the second day at the 2–4th stages of cellular embryo, and the quality of obtained embyos was evaluated on day 8. Based on the statistical analysis of the data, we determined that the artificial activation of bovine oocytes using calcium ionphore after intracytoplasmic spermatozoid injection (ICSI) led to statistically significant improvement in conception and ratio of blastocytes obtained to oocytes injected. In the study, we confirmed that addition of 5, 10 and 50 µМ of the agent had the same efficacy on the activation of occytes of bovine cattle. However, it has to be noted that during further cultivation of the obtained zygotes up to the blastocyte stage (day 8), we saw no significant differences in quality of embryos obtained. Therefore, use of calcium ionophore for the activation of bovine oocytes after intracytoplasmic spermatozoid injection is effective, for it promotes increase in fertilization parameters and ratio of blastocytes obtained to oocytes injected, facilitating production of higher numbers of embyos suitable for transplantation or cooling. Our previous conclusions are valuable for increasing the efficacy of methods of intracytoplasmic injection of bovine spermatozoid and its further use for purposes of science and production.

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“…Nevertheless, it was proven that ICSI can successfully represent a viable alternative when immature or suboptimal quality sperm was used in several species [4][5][6][7]. Therefore, ICSI could allow the use of lower quality semen, collected from younger bulls, as well as sexed semen for successful fertilization of oocytes [8].…”

Section: Introductionmentioning

confidence: 99%

Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows

et al. 2021

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(1) Background: Intracytoplasmic sperm injection (ICSI) is currently used to increase fertilization success by avoiding several oocyte or sperm deficiencies that would normally prevent conception after in vivo fertilization or classical in vitro fertilization. This paper aimed at improving the in vitro fertilization protocol of bovine oocytes, harvested from old cows after slaughtering, using intracytoplasmic sperm injection; (2) Methods: Oocytes were harvested by puncture of follicles from ovaries obtained from slaughtered old cows, followed by aspiration. Out of the 127 cumulus-oocyte complexes that were harvested, 84 (66.14%) were declared suitable for cultivation, after morphological evaluation. Following oocyte maturation for 22 hours, 77 cumulus-oocyte complexes were morphologically intact and could undergo the steps required for intracytoplasmic injection of spermatozoa. Frozen-thawed bull semen was used for ICSI and the 77 fertilized oocytes were kept for 24 hours in an atmosphere enriched with 5% CO2.; (3) Results: Fertilized oocytes transformed into 46 zygotes (fertilization rate of 59.74%), while after 168 h of cultivation 38 transferable compact morulae or early blastocysts were obtained; (4) Conclusions: Intracytoplasmic sperm injection can represent a viable alternative to classical IVF, when oocytes or sperm with lower fertility are used.

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Intracytoplasmic Sperm Injection in Cattle

Cited by 11 publications

References 163 publications

SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1

SPERM FACTORS AND EGG ACTIVATION: ICSI and the discovery of the sperm factor and PLCZ1

Influence of calcium ionophore on the fertilization of bovine oocytes and their further embryonic development

Production of viable bovine embryos by intracytoplasmic sperm injection of oocytes harvested from slaughtered old cows

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