Improved enzyme retention from an electropolymerized polypyrrole-alginate matrix in the development of biosensors

Enzyme-based Electrochemical Biosensors

“…Alginate hydrogel supports are usually made by cross-linking the carboxyl group of the guluronic acid residue with a solution of cationic crosslinkers such as calcium choloride, barium chloride, strontium, etc., and thus enzyme could retain their activity in alginate hydrogels (Martinsen et al, 1989). By taking advantages of both of alginate and PPy, a novel composite was synthesized through providing a gel by Ca + cross-linking (Ionescu et al, 2005), which exhibitis a greater enzyme retention as well as increased alginate stability towards the destructive effect of phosphate anions compared to the natural alginate gel. Recently, protonated sodium alginate (pSA) was also reported to be a dopant for electrogeneration of Ppy/pSA functionalized films for GOD immobilization.…”

Amperometric Algal Chlorella vulgaris Cell Biosensors Based on Alginate and Polypyrrole‐Alginate Gels

“…Since the conductometric biosensors [14] based on entrapment of algae cells Chlorella vulgaris into regular alginates gels have displayed a very limited operational stability, a novel synthetic alginate molecule with better molecular retention properties was designed [19,20]. This molecule is based on grafting pyrrole moieties on alginate which then will allow for a controllable electropolymerization process and a new and putatively more stable configuration on the electrode surface so created.…”

Development of Highly Sensitive Amperometric Biosensor for Glucose Using Carbon Nanosphere/Sodium Alginate Composite Matrix for Enzyme Immobilization

“…20,21 Alginate displays several properties including biocompatibility, film-forming ability and gentle microenvironments to keep stability of the enzyme immobilized in the entrapped material. 22 In this paper, we developed a composite matrix based on CNS and sodium alginate (SA) to design a highly sensitive glucose biosensor.…”