Improved Enzyme Activity and Stability in Polymer Microspheres by Encapsulation of Protein Nanospheres

Montalvo-Ortiz, Brenda L.; Sosa, Brian; Griebenow, Kai

doi:10.1208/s12249-012-9794-3

Cited by 16 publications

(14 citation statements)

References 19 publications

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“…The HRP/MβCD powder was suspended in ethyl acetate in order to dissolve MβCD followed by sonication for 30 s. The solid protein nanoparticles were collected by centrifugation following removal of ethyl acetate containing MβCD. The formed nanoparticles were encapsulated into PLGA microspheres by s/o/w method [66]. Investigators have also prepared lyophilized HRP loaded PLGA microspheres.…”

Section: Colloidal Delivery Systemsmentioning

confidence: 99%

Recent Developments in Protein and Peptide Parenteral Delivery Approaches

2014

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Discovery of insulin in the early 1900s initiated the research and development to improve the means of therapeutic protein delivery in patients. In the past decade, great emphasis has been placed on bringing protein and peptide therapeutics to market. Despite tremendous efforts, parenteral delivery still remains the major mode of administration for protein and peptide therapeutics. Other routes such as oral, nasal, pulmonary and buccal are considered more opportunistic rather than routine application. Improving biological half-life, stability and therapeutic efficacy is central to protein and peptide delivery. Several approaches have been tried in the past to improve protein and peptide in vitro/in vivo stability and performance. Approaches may be broadly categorized as chemical modification and colloidal delivery systems. In this review we have discussed various chemical approaches such as PEGylation, hyperglycosylation, mannosylation, and colloidal carriers including microparticles, nanoparticles, liposomes, carbon nanotubes and micelles for improving protein and peptide delivery. Recent developments on in situ thermosensitive gel-based protein and peptide delivery have also been described. This review summarizes recent developments on some currently existing approaches to improve stability, bioavailability and bioactivity of peptide and protein therapeutics following parenteral administration.

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Section: Colloidal Delivery Systemsmentioning

confidence: 99%

Recent Developments in Protein and Peptide Parenteral Delivery Approaches

2014

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“…170 The increased size of the enzymecage complex compared with the enzyme alone can also facilitate reuse of enzymes in industry. 171 Examples of non-proteinbased nanosized cages are polymers, 172,173 polymersomes, 174,175 liposomes, 176,177 giant amphiphiles, 178 nanogels, 179 layer-by-layer structures, 180 nanodroplets, 181 nanosized metal organic frameworks (MOF), 182 silica nanoparticles 183 and other inorganic cages. 184,185 Most of these systems are especially applicable when stability is needed in extreme environments, as in the case of water-free systems, or when high (thermal) stability is required.…”

Section: Advantages Of Protein Nanoreactors Over Other Nanoreactorsmentioning

confidence: 99%

Structural nanotechnology: three-dimensional cryo-EM and its use in the development of nanoplatforms forin vitrocatalysis

et al. 2019

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“…Encapsulation efficiency % ð Þ= mass extracted protein mass theoretical mass loading × 100 , where mass extracted protein is the mass of protein recovered per mass of PLGA microparticles after removal of PLGA via organic solvent and mass theoretical mass loading is the theoretical maximum mass of protein per mass of PLGA microparticles. Loading levels of proteins within PLGA microparticles are typically 1-10 wt% and the encapsulation efficiency using emulsion-based production can vary from 30 to 95% (Emami et al, 2009;Li, Anderson, Mehta, & Deluca, 1995;Lim, Lee, Widjaja, & Loo, 2013;Montalvo-Ortiz, Sosa, & Griebenow, 2012). The encapsulation efficiency of peptides and proteins is dependent on the physicochemical characteristic of the protein and stabilization of the primary and secondary emulsion.…”

Section: Double Emulsionmentioning

confidence: 99%

Poly(lactic‐co‐glycolic acid) devices: Production and applications for sustained protein delivery

Lee

Pokorski

2018

WIREs Nanomed Nanobiotechnol

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Injectable or implantable poly(lactic-co-glycolic acid) (PLGA) devices for the sustained delivery of proteins have been widely studied and utilized to overcome the necessity of repeated administrations for therapeutic proteins due to poor pharmacokinetic profiles of macromolecular therapies. These devices can come in the form of microparticles, implants, or patches depending on the disease state and route of administration. Furthermore, the release rate can be tuned from weeks to months by controlling the polymer composition, geometry of the device, or introducing additives during device fabrication. Slow-release devices have become a very powerful tool for modern medicine. Production of these devices has initially focused on emulsion-based methods, relying on phase separation to encapsulate proteins within polymeric microparticles. Process parameters and the effect of additives have been thoroughly researched to ensure protein stability during device manufacturing and to control the release profile. Continuous fluidic production methods have also been utilized to create protein-laden PLGA devices through spray drying and electrospray production. Thermal processing of PLGA with solid proteins is an emerging production method that allows for continuous, high-throughput manufacturing of PLGA/protein devices. Overall, polymeric materials for protein delivery remain an emerging field of research for the creation of single administration treatments for a wide variety of disease. This review describes, in detail, methods to make PLGA devices, comparing traditional emulsion-based methods to emerging methods to fabricate protein-laden devices. This article is categorized under: Biology-Inspired Nanomaterials > Protein and Virus-Based Structures Implantable Materials and Surgical Technologies > Nanomaterials and Implants Biology-Inspired Nanomaterials > Peptide-Based Structures.

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Improved Enzyme Activity and Stability in Polymer Microspheres by Encapsulation of Protein Nanospheres

Cited by 16 publications

References 19 publications

Recent Developments in Protein and Peptide Parenteral Delivery Approaches

Recent Developments in Protein and Peptide Parenteral Delivery Approaches

Structural nanotechnology: three-dimensional cryo-EM and its use in the development of nanoplatforms forin vitrocatalysis

Poly(lactic‐co‐glycolic acid) devices: Production and applications for sustained protein delivery

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