Improved dimensional stability with bioactive glass fibre skeleton in poly(lactide-co-glycolide) porous scaffolds for tissue engineering

Haaparanta, Anne-Marie; Uppstu, Peter; Hannula, Markus; Ellä, Ville; Rosling, Ari; Kellomäki, Minna

doi:10.1016/j.msec.2015.07.013

Cited by 27 publications

(22 citation statements)

References 33 publications

(43 reference statements)

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“…In the present study, BG alone resulted in adequate bone formation, but combining PLGA with BGf deteriorated the repair process. PLGA–BGf had initially small pores and compact structure with very little space for tissue ingrowth (Haaparanta et al, ). The BGfs were densely embedded in the PLGA, which probably impaired the interaction of BGf with the surrounding bone.…”

Section: Discussionmentioning

confidence: 99%

“…The BGfs were densely embedded in the PLGA, which probably impaired the interaction of BGf with the surrounding bone. As the composite material has a longer degradation time than the PLGA alone, it might lead to better structural support in load‐bearing applications (Gentile et al, ) but delay the lesion repair (Haaparanta et al, ). We believe these factors explain why the bone defects treated with the PLGA–BGf composite scaffold were only filled with connective tissue and why the bone structure remained nearly unchanged throughout the 3‐month long study period.…”

Section: Discussionmentioning

confidence: 99%

“…The freeze‐dried PLGA–BGf composites were afterwards cut with a puncher into samples with diameter of 4 mm, and five parallel samples were placed on top of each other and glued together with 3 wt‐% PLGA solution and freeze‐dried again as described earlier. The height of the final sample was 8 mm, with a porosity of 96% (Haaparanta et al, ). The samples were held under vacuum at room temperature for a minimum of 48 hr and gamma sterilized at 25 kGy.…”

Section: Methodsmentioning

confidence: 99%

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Gas‐foamed poly(lactide‐co‐glycolide) and poly(lactide‐co‐glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defects

Salonius

Muhonen

Lehto

et al. 2019

J Tissue Eng Regen Med

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Deep osteochondral defects may leave voids in the subchondral bone, increasing the risk of joint structure collapse. To ensure a stable foundation for the cartilage repair, bone grafts can be used for filling these defects. Poly(lactide-co-glycolide) (PLGA) is a biodegradable material that improves bone healing and supports bone matrix deposition. We compared the reparative capacity of two investigative macroporous PLGAbased biomaterials with two commercially available bone graft substitutes in the bony part of an intra-articular bone defect created in the lapine femur. New Zealand white rabbits (n = 40) were randomized into five groups. The defects, 4 mm in diameter and 8 mm deep, were filled with neat PLGA; a composite material combining PLGA and bioactive glass fibres (PLGA-BGf); commercial beta-tricalcium phosphate (β-TCP) granules; or commercial bioactive glass (BG) granules. The fifth group was left untreated for spontaneous repair. After three months, the repair tissue was evaluated with X-ray microtomography and histology. Relative values comparing the operated knee with its contralateral control were calculated. The relative bone volume fraction (ΔBV/TV) was largest in the β-TCP group (p ≤ 0.012), which also showed the most abundant osteoid. BG resulted in improved bone formation, whereas defects in the PLGA-BGf group were filled with fibrous tissue. Repair with PLGA did not differ from spontaneous repair. The PLGA, PLGA-BGf, and spontaneous groups showed thicker and sparser trabeculae than the commercial controls. We conclude that bone repair with β-TCP and BG granules was satisfactory, whereas the investigational PLGAbased materials were only as good as or worse than spontaneous repair.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Gas‐foamed poly(lactide‐co‐glycolide) and poly(lactide‐co‐glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defects

Salonius

Muhonen

Lehto

et al. 2019

J Tissue Eng Regen Med

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“…The stability of the implanted scaffolds in vivo will be beneficial for performing their performances and providing adequate space for newborn tissues. 34,35 Generally, the stability of the scaffolds could be evaluated by detecting the degradation behavior in vitro using related simulated body fluid, such as PBS. Therefore, the degradation of various PCL scaffolds was evaluated by PBS immersion for 30 days in the present study.…”

Section: Stability Of Pcl Scaffoldsmentioning

confidence: 99%

Fabrication of alignment polycaprolactone scaffolds by combining use of electrospinning and micromolding for regulating Schwann cells behavior

Zhang

Chen

Liang

et al. 2018

J Biomedical Materials Res

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In the present study, a new approach for fabricating micropatterned polycaprolactone (PCL) scaffolds with ridge/groove structure on the surface was developed by combining use of electrospinning and micromolding method. A series of physicochemical properties, including morphology, wettability, component, crystal pattern and mechanical properties, of prepared PCL scaffolds were characterization, respectively. Stability of the micropatterned PCL scaffolds was measured using phosphate buffer solution immersion for a certain period. Then, the regulating effects of the micropatterned PCL scaffolds on attachment, orientation and normal biological function of Schwann cells were evaluated. And the protein adsorption behavior in various PCL scaffolds was also detected. The results showed that the micropatterned PCL scaffolds demonstrated a porous micro/nano complex structure with enhanced hydrophobicity and mechanical properties as a function of electrospun flow-rate of PCL solution. The micropatterned PCL scaffolds possessed good stability and could effectively regulate the attachment and orientation of Schwann cells at the early stage after cell culture. Importantly, the electrospun flow-rate of PCL solution was found to play an important role in scaffold properties, cell behavior and protein adsorption. The micropatterned scaffolds with a flow-rate of PCL solution at 0.12 mL h demonstrated the better regulation on Schwann cells attachment and alignment without negatively affect the normal biological function of the cells. To the best of our knowledge, this is the first report of combining use of electrospinning and micromolding method for preparing artificial nerve implants. The study is anticipated to have potential application in peripheral nerve and other tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3123-3134, 2018.

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“…Biological scaffolds, as a fundamental factor in tissue engineering, can help to guide cell growth, secretion of extracellular cell matrix and facilitate the formation of functional tissues and organs (Celiz et al 2014;Kedong et al 2014;Ye et al 2014;Ban et al 2015;Haaparanta et al 2015). Scaffolds also play an important role in repairing bone defect by fabricating three-dimensional (3D) engineered bone tissues in vitro (McNamara et al 2014;Yasui et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Repair of bone defects using dynamic fabricated tissue-engineered bone based on a bio-derived porous bone scaffold

Song

et al. 2016

Animal Cells and Systems

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Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1 × 10 6 cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin-eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect. ARTICLE HISTORY

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Improved dimensional stability with bioactive glass fibre skeleton in poly(lactide-co-glycolide) porous scaffolds for tissue engineering

Cited by 27 publications

References 33 publications

Gas‐foamed poly(lactide‐co‐glycolide) and poly(lactide‐co‐glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defects

Gas‐foamed poly(lactide‐co‐glycolide) and poly(lactide‐co‐glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defects

Fabrication of alignment polycaprolactone scaffolds by combining use of electrospinning and micromolding for regulating Schwann cells behavior

Repair of bone defects using dynamic fabricated tissue-engineered bone based on a bio-derived porous bone scaffold

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