Improved development by melatonin treatment after vitrification of mouse metaphase II oocytes

Zhang, Yue; Li, Wei; Ma, YingLiang; Wang, Dian; Zhao, Xiaozhen; Zeng, Changjun; Zhang, Ming; Zeng, Xianyin; Meng, Qinggang; Zhou, Guangbin

doi:10.1016/j.cryobiol.2016.09.171

Cited by 33 publications

(47 citation statements)

References 57 publications

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“…As a potent antioxidant, melatonin detoxifies ROS including OH -, O 2and H 2 O 2 and reduces the oxidative damage of ovarian cells [23] and thus, improves the fertility and fecundity of sheep by improving the survival rate of corpus luteum and embryos [24]. Numerous studies have proved that melatonin promotes the development of oocytes and embryos in sheep [25], pigs [26], cattle [27], mice [28] and humans [29] in the in vitro environment by scavenging ROS or. Luridiana et al reported that the melatonin implantation in ewe at the age of 5-6 with 3.5-4.0 body condition score (BSC) in spring improved fertility of ewes [30].…”

Section: Discussionmentioning

confidence: 99%

Peer Review #3 of "Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep (v0.2)"

Deng¹

2019

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It has been well proved that melatonin participates in the regulation of the seasonal reproduction of ewes. However, the effects of short term treatment of melatonin on ewe's ovulation are still to be clarified. In this study, the effects of melatonin on the number of embryos harvested from superovulation, and the pregnant rate in recipients after embryo transferred have been investigated. Hu sheep with synchronous estrus treatment were given melatonin subcutaneously injection (0, 5, and 10 mg/ewe, respectively). It was found that the estrogen level in the group of 5 mg melatonin was significantly higher than that of other two groups at the time of sperm insemination (p < 0.05). The pregnant rate and number of lambs in the group of 5 mg melatonin treatment was also significantly higher than that of the rests of the groups (P < 0.05). In another study, 31 Suffolk ewes as donors and 103 small-tailed han sheep ewes as recipients were used to produce pronuclear embryo and embryo transfer. Melatonin (5 mg) was given to the donors during estrus. The results showed that, the number of pronuclear embryos and the pregnancy rate were also significantly higher in melatonin group than that in the control group. In addition, 28 donors and 44 recipient ewes were used to produce morula/blastocyst and embryo transferring. Melatonin (5 mg) was given during estrus. The total number of embryos harvested (7.40±1.25/ewe vs. 3.96±0.73/ewe, P<0.05) and the pregnant rate (72.3±4.6% vs.54.7±4.0%, P<0.05) and number of lambs were also increased in melatonin group compared to the control group. Collectively, the results have suggested that melatonin treatment 36 hours after CIDR withdrawal could promote the number and quality of embryos in the in vivo condition and increased the pregnant rate and number of lambs.

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Section: Discussionmentioning

confidence: 99%

Peer Review #3 of "Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep (v0.2)"

Deng¹

2019

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“…Moreover, it also has offered a convenient way to protect endangered wildlife germplasm resources [8] and to build superior breeding pools for livestock [9,10]. However, the survival rate of oocytes and their subsequent developmental competence are decreased significantly after vitrification when compared with these characteristics of fresh oocytes [11,12,13]. The decreased developmental potential due to oocyte cryopreservation may inevitably result from the alteration of intracellular levels of reactive oxygen species (ROS) [14] or glutathione (GSH) [15], and/or gene expression [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, ROS and GSH are pivotal to maintaining the level of reductants and oxidants in a balanced state [25] in order to regulate oocyte maturation and normal development of zygotes. After oocytes are subjected to vitrification and warming, ROS levels are generally increased [13,14] and, conversely, GSH levels tend to decline [15,26,27]. In such situations, redox homeostasis would be perturbed, potentially weakening the quality of oocytes and reducing their developmental competence [28].…”

Section: Introductionmentioning

confidence: 99%

“…Melatonin (MT), a scavenger of ROS, can promote oocyte maturation in vitro and enhance the rate of blastocyst formation of embryos cultured in vitro [43,44,45]. In this regard, in our previous study, we reported that 10 −9 mol/L MT supplementation during vitrification/warming, activation and in vitro culture could increase the development potential of vitrified–warmed mouse metaphase II (MII) oocytes [13]. Meanwhile, previously MT was found to play an important role in cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Pan

Yang

et al. 2018

IJMS

Self Cite

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This study aimed to investigate the effect of melatonin on the cell cycle of parthenogenetic embryos derived from vitrified mouse metaphase II (MII) oocytes. Fresh oocytes were randomly allocated into three groups: untreated (control), or vitrified by the open-pulled straw method without (Vitrification group) or with melatonin (MT) supplementation (Vitrification + MT group). After warming, oocytes were parthenogenetically activated and cultured in vitro, then the percentage of embryos in the G1/S phase, the levels of reactive oxygen species (ROS) and glutathione (GSH), and the mRNA expression of cell cycle-related genes (P53, P21 and E2F1) in zygotes and their subsequent developmental potential in vitro were evaluated. The results showed that the vitrification/warming procedures significantly decreased the frequency of the S phase, markedly increased ROS and GSH levels and the expression of P53 and P21 genes, and decreased E2F1 expression in zygotes at the G1 stage and their subsequent development into 2-cell and blastocyst stage embryos. However, when 10−9 mol/L MT was administered for the whole duration of the experiment, the frequency of the S phase in zygotes was significantly increased, while the other indicators were also significantly improved and almost recovered to the normal levels shown in the control. Thus, MT might promote G1-to-S progression via regulation of ROS, GSH and cell cycle-related genes, potentially increasing the parthenogenetic development ability of vitrified–warmed mouse oocytes.

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“…Mouse Oocytes 10 -9 to 10 -3 M after in vitro maturation Increases in vitro fertilization rate, reduces ROS and inhibits apoptosis [72] Bovine Zygotes 1 µM for 3 hours after insemination and at 40°C Reduces ROS levels in embryos [73] Mouse Oocyte M-II 10 -9 mol/L during vitrification/warming and PA Increases blastocyst rate after warming, compared with control group. [74] Bovine Sperm 10 -3 M for 3 hours before in vitro fertilization Improves the plasma membrane and acrosome integrity, mitochondrial activity and it decreases intracellular ROS levels. Also, it increases the blastocyst rate and it decreases apoptosis rate.…”

Section: Melatoninmentioning

confidence: 99%

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on <em>In Vitro</em> Embryo Production in Mammals

Loren¹,

Sánchez²,

Arias³

et al. 2017

Preprint

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Oxidative and nitrosative stress are a common problem when manipulating gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because its absence or over production causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during IVF has deleterious or positive effects depending on the concentration used in culture medium, demonstrating the delicate balance that must exist between antioxidant and pro-oxidant activity. Further research is needed to better understand the possible impact of melatonin on the different IVP steps in domestic animals, especially in seasonal breeds where this neuro-hormone system highly regulates its reproduction physiology.

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Improved development by melatonin treatment after vitrification of mouse metaphase II oocytes

Cited by 33 publications

References 57 publications

Peer Review #3 of "Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep (v0.2)"

Peer Review #3 of "Melatonin improves the efficiency of super-ovulation and timed artificial insemination in sheep (v0.2)"

Melatonin Improves Parthenogenetic Development of Vitrified–Warmed Mouse Oocytes Potentially by Promoting G1/S Cell Cycle Progression

Melatonin Scavenger Properties against Oxidative and Nitrosative Stress: Impact on <em>In Vitro</em> Embryo Production in Mammals

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