“…The detection rates of p24 in the sera of HIV-1-seropositive individuals by the conventional two-site enzyme immunoassays were lower than those by the present enzyme immunoassay. The detection rates by the conventional two-site enzyme immunoassays before and after acid treatment of serum were 8 to 10% (4,17,21) and 38 to 48% (17,21), respectively, for asymptomatic carriers, 12% (21) and 59% (21), respectively, for patients with ARC, and 37 to 86% (4,15,21) and 86% (21), respectively, for patients with AIDS. By the present enzyme immunoassay, the detection rate for the asymptomatic carriers was 48% before acid treatment and improved to 68% with acid treatment, and the detection rates for the patients with ARC and AIDS were 78 and 90%, respectively, with no improvement with acid treatment.…”